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:: Volume 26, Issue 3 (fall 2016) ::
MEDICAL SCIENCES 2016, 26(3): 166-172 Back to browse issues page
Differentiation of induced pluripotent stem cells into bone and adipose cell lineages
Elham Hoveizi *1, Tayebeh Mohammadi2
1- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , e.hoveizi@scu.ac.ir
2- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (2939 Views)

Background: Human induced pluripotent cells (hiPSCs) could be established as promising new resources for obtaining differentiated cells for cell therapy. iPSCs have the potential to use as multipurpose research and clinical tools to understand model diseases, develop and screen candidate drugs, and deliver cell-replacement therapy to support regenerative medicine. Here, we demonstrated the ability of human iPSCs to differentiate into adipocyte and osteoblast fate.

Materials and methods: In this experimental study, human iPSCs were culture-expanded. HiPSCs were then cultivated in the osteogenic and adipogenic conditions for 21 days after which differentiations were evaluated by specific staining as well as qRT-PCR analysis for related gene expression.

Results: In osteoinductive cultures, the cells formed nodules which were positively stained red following alizarin red staining. In adipogenic cultures, the cells developed some lipid droplets which were positively stained red with oil red. According to qRT-PCR analysis, the bone-related genes including osteocalcin and osteopontin, and also the adipocyte-specific genes such as PPAR and LPL were expressed in the osteogenic and adipogenic cultures, respectively.

Conclusion: Taken together, hiPSCs are pluripotent cells with the ability to differentiate into osteocytic and adipocytic cell lineages.

Keywords: Differentiation, Induced pluripotent stem cells, Bone and adipose cells.

Keywords: Differentiation, Induced pluripotent stem cells, Bone and adipose cells.
Full-Text [PDF 421 kb]   (921 Downloads)    
Semi-pilot: Experimental | Subject: Molecular Biology
Received: 2015/08/10 | Accepted: 2015/12/27 | Published: 2016/09/17
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Hoveizi E, Mohammadi T. Differentiation of induced pluripotent stem cells into bone and adipose cell lineages. MEDICAL SCIENCES. 2016; 26 (3) :166-172
URL: http://tmuj.iautmu.ac.ir/article-1-1131-en.html


Volume 26, Issue 3 (fall 2016) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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