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Association of miR-196a2 (rs11614913) polymorphism with colorectal cancer in Tehran population
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Hanieh Mirtalebi1   , Mitra Heydari Nasrabadi2 , Mohamad Amin Pourhoseingholi3 , Hamid Asadzadeh-Aghdaei4 |
1- MSc of Cellular and Developmental Biology, Department of Biology, Islamic Azad University, Parand Branch, Tehran, Iran
2- PhD of Cellular and Developmental Biology, Department of Biology, Islamic Azad University, Parand Branch, Tehran, Iran , heydari_nasr@yahoo.com
3- PhD of Biostatistics, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4- Gastroenterologist, Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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Abstract: (10393 Views) |
Background: Micro RNAs (miRNAs) are identified agents for post-transcriptional regulation of gene expression and miRNA polymorphisms are recognized as useful biomarkers for prognosis of colorectal cancer. The association of rs 11614913 polymorphism in miR-196a2 gene and the risk of colorectal cancer were investigated in this study.
Materials and Methods: I this case- control study, 149 patients with colorectal cancer and 146 healthy subjects whom referred to Taleghani hospital, Tehran were studied. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method was used for genotyping.
Results: The frequency of CT, TT and CC genotypes in control group were 40%, 24% and 36%, respectively. Moreover, genotype frequencies in case group were revealed as 49% for CT, 10% for TT and 41 % for CC. The frequencies of C and T alleles in control group were 48% and 70%, and 42% and 30% in case group, respectively. Among all genotypes, an association between CRC risk and TT genotype was determined in our survey (OR=0.4, 95%CI= 0.17-0.92 p=0.03).
Conclusion: This study showed that modified miR-196a2 sequence affects microRNA efficiency which may have an impact on colorectal cancer susceptibility. |
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| Keywords: Polymorphism, MicroRNA, Colorectal cancer |
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Full-Text [PDF 194 kb]
(2547 Downloads)
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Semi-pilot: case-control |
Subject:
Molecular Biology
Received: 2014/01/22 | Accepted: 2014/01/22 | Published: 2014/01/22
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