fa اثر اسید گالیک بر شاخص‌های استرس اکسایشی و تراکم سلولی هیپوکامپ در مدل ایسکمی- خونرسانی مجدد کلیوی زيست شناسي جانوري Animal Biology تجربي Experimental <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">هرچند، آسیب حاد کلیوی (</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">AKI</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">) از پیامدهای ایسکمی- خونرسانی مجدد کلیوی (</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">RIR</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">) است، آسیب اکسیداتیو ناشی از </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">RIR</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> بر اندام‌های دور نیز اثر می‌گذارد. هدف از پژوهش حاضر ارزیابی اثر اسید گالیک بر شاخص‌های استرس اکسیداتیو و&nbsp; تراکم نورونی هیپوکامپ مغز به دنبال </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">RIR</span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> بود. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">48 سر موش صحرایی نر نژاد ویستار به طور تصادفی در 4 گروه کنترل، ایسکمی/ریپرفیوژن+‌نرمال سالین (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR+Saline</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)، ایسکمی/ریپرفیوژن+‌اسید گالیک با دوزهای 100 (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR+GA100</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) و 200 میلی گرم (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR+GA200</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) تقسیم شدند. حیوانات در تمامی گروه‌ها به جز کنترل، مورد نفروکتومی یک طرفه (سمت راست) قرار گرفتند. تیمار به مدت 14 روز انجام شد و سپس، کلیه سمت چپ به مدت 45 دقیقه مورد ایسکمی قرار گرفت</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> 72 ساعت بعد، سطح فعالیت هیپوکامپی آنزیم کاتالاز (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)، گلوتاتیون پراکسیداز (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">GPx</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)، ظرفیت آنتی اکسیدانی تام (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) و مالون دی آلدهید (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) ارزیابی شد و در پایان، تراکم نورونی نواحی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA3</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> هیپوکامپ سنجیده شد.</span></span></span></span></i><i>&nbsp; </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">در گروه</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR+Saline</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> کاهش معنی‌دار </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">،</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">GPx </span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&nbsp;و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و افزایش </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> نسبت به گروه کنترل دیده شد که با کاهش معنی‌دار تراکم نورونی در نواحی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1/CA3</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> همراه بود (05/0></span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در حالی که گروه‌های دریافت کننده اسید گالیک همراه با افزایش معنی‌دار </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">GPx</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و کاهش میزان </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در هیپوکامپ افزایش معنی‌دار تراکم نورونی در نواحی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1/CA3</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> را نسبت به گروه </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR+Saline</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> نشان دادند (05/0</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span arial="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">˂</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). </span></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">&shy;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">پیش درمان با اسید گالیک می‌تواند آسیب اکسیداتیو و مرگ نورونی را در هیپوکامپ مغز موش‌های صحرایی دچار آسیب حاد کلیوی ناشی از </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">RIR</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> کاهش دهد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Although acute renal injury (AKI) is a consequence of renal ischemia-reperfusion (RIR), RIR-induced oxidative damage also affects distant organs. The aim of this study was to evaluate the effect of gallic acid (GA) on oxidative stress parameters and neuronal density in the brain hippocampus following RIR. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: 48 male Wistar rats were randomly divided into 4 groups, including control, ischemia/reperfusion+normal saline (RIR+Saline), and ischemia/reperfusion+GA groups at doses of 100 mg/kg (RIR+GA100) and 200 mg/kg (RIR+GA200). Animals in all groups except control underwent unilateral nephrectomy (right). The treatments were performed for 14 days and then, the left kidney was ischemized for 45 minutes. After 72 hours, hippocampal activity levels of catalase (CAT), glutathione peroxidase (GPx), total antioxidant capacity (TAC) and malondialdehyde (MDA) were evaluated. Finally, neuronal density was measured in CA1 and CA3 regions of the hippocampus. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: A significant decrease in CAT, GPx and TAC and an increase in MDA was observed in RIR + Saline group compared to the control group, which was associated with a significant decrease in neuronal density in CA1/CA3 (p<0.05). While in the GA-treated groups, along with a significant increase in CAT, GPx and TAC and a decrease in MDA in the hippocampus, showed a significant increase in neuronal density of CA1/CA3 regions compared to the RIR+Saline group (p˂0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: GA pretreatment can ameliorate oxidative damage and neuronal death in the brain hippocampus of rats with RIR-induced acute renal damage.</span></span></span></span></span></span><br> &nbsp; اسید گالیک, هیپوکامپ, آسیب حاد کلیوی, موش صحرایی Gallic acid, Hippocampus, Acute renal failure, Rat 379 388 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-727-8&slc_lang=fa&sid=1 Hossein Roshanfekr حسین روشنفکر hroshanfekr5@gmail.com 100319475328460010848 100319475328460010848 No PhD Candidate in Animal Physiology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran دانشجوی دکتری فیزیولوژی جانوری،گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران Mohammad Amin Edalatmanesh محمد امین عدالت منش amin.edalatmanesh@gmail.com 100319475328460010849 100319475328460010849 Yes دانشیار،فیزیولوژی، گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز Heydar Aghababa حیدر آقابابا heydar2001@yahoo.com 100319475328460010850 100319475328460010850 No of Physiology, Department of Biology, College of Sciences, Arsanjan Branch, Islamic Azad University, Shiraz, Iran استادیار، فیزیولوژی، گروه زیست شناسی، دانشکده علوم، واحد ارسنجان، دانشگاه آزاد اسلامی، ارسنجان، ایران