Medical Sciences Journal of Islamic Azad University
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی تهران
MEDICAL SCIENCES
Medical Sciences
http://tmuj.iautmu.ac.ir
1
admin
1023-5922
2008-3386
000
10.61882/iau
000
1023-5922
000
fa
jalali
1403
7
1
gregorian
2024
10
1
34
3
online
1
fulltext
fa
بررسی اثر ضد بیوفیلمی ترکیبات شبه باکتریوسینی تولید شده توسط باسیلوس سوبتیلیس SP1 جدا شده از عسل سبلان
Anti-biofilm effects of bacteriocin-like compounds produced by Bacillus subtilis SP1 isolated from Sablan honey
ميكروبيولوژي
Microbiology
تجربي
Experimental
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">فرایند</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">درمان عفونت­های ناشی ازباکتری</span></span></span></i><i><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">های مولد بیوفیلم سخت وپیچیده است و نیاز به استفاده از</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">ترکیباتی</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal"> جدید برای درمان این نوع عفونت</span></span></span></i><i><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">ها وجود دارد. این پژوهش با هدف</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal"> بررسی</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">ترکیبی موثر ب</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">رای</span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">مهار بیوفیلم <a name="_Hlk156747240">باکتری های </a></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">سودوموناس آئروژینوزا، </span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">اشرشیاکلای، استرپتوکوکوس موتانس و استافیلوکوکوس اورئوس انجام شد</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">در این مطالعه اثر غلظت</span></span></span></span></i><i><span lang="AR-SA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">های مختلف ترکیبات شبه باکتریوسینی تولید شده توسط باسیلوس سوبتیلیس </span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">SP1</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> جدا شده از عسل سبلان </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">جهت مهار بیوفیلم حاصل از </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">باکتری های </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">سودوموناس آئروژینوزا، </span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">اشرشیاکلای، استرپتوکوکوس موتانس و استافیلوکوکوس اورئوس با استفاده از میکروپلیت 96 خانه </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">و رنگ آمیزی با کریستال ویوله </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">بررسی شد</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></span><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">­</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">درصد </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">خاصیت ضد بیوفیلمی</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> این ترکیبات در برابر </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">S. mutans</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">،</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">S. aureus </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> ، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">E. coli</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> P. aeruginosa</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در غلظت </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> µg/ml</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">80 به ترتیب معادل 80 ،62 ،50 و 15 بود . این درصد برای </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">S. mutans</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">غلظت </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> µg/ml</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">009/0 به 2% و برای </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">S. aureus </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> درغلظت </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">µg/ml </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> 156/0 به </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">5% </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">رسید. برای </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">E. coli</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P. aeruginosa</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در همین غلظت به </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> ترتیب به 2% و</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> 1% کاهش یافت</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتیجه</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">بیشترین درصد خاصیت ضد بیوفیلمی دربرابر </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">S. mutans</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> وکمترین درصد در برابر </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P. aeruginosa </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> مشاهده شد.</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Koodak""><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Biofilms are communities of microorganisms which are difficult and complicated to treat with antimicrobial agents. Therefore, there is a need to use new compounds to treat these types of resistant microorganisms. This study was done with the aim of finding an effective compound for inhibition of biofilms of <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Streptococcus mutans</i>, and <i>Staphylococcus aureus</i>. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: The effect of bacteriocin-like compounds produced by <i>B. subtilis</i> SP1 isolated from Sablan native honey on the biofilm of four bacterial strains including <i>E. coli</i>, <i>P. aeruginosa</i>, <i>St. mutans </i>and <i>S. aureus</i> was investigated by using 96-well microtiter plate and crystal violet staining. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: The percentage of anti-biofilm effect against <i>S. mutans</i> and <i>S. aureus</i>, <i>E. coli</i> and <i>P. aeruginosa</i> at 80 µg/ml of bacteriocin like compounds were about 80, 62, 50, and 15 percent, respectively. The antibiofilm effect was concentration dependent and reduced at lower concentrations of bacteriocin like compounds.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: The highest percentage of anti-biofilm effect of bacteriocin like compounds was observed against <i>S. mutans</i> and the lowest percentage of inhibition was against <i>P. aeruginosa</i>.</span></span></span></span></span></span><br>
بیوفیلم, باسیلوس سوبتی لیس, باکتریوسین
بیوفیلم, باسیلوس سوبتی لیس, باکتریوسین
237
244
http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-1780-1&slc_lang=fa&sid=1
Maryam
Khalili Samani
مریم
خلیلی سامانی
maryamkhalili1983@hotmail.com
100319475328460012546
0009-0009-8679-0624
No
PhD in Microbiology, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
دکتری میکروبیولوژی، گروه میکروبیولوژی ،دانشکده علوم پایه دانشگاه آزاد اسلامی، واحد علوم وتحقیقات، تهران، ایران
Zahra
Noormohammadi
زهرا
نورمحمدی
marjannm@yahoo.com
100319475328460012547
100319475328460012547
No
Professor of Genetics, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
استاد ِژنتیک، گروه زیست شناسی، دانشکده علوم پایه دانشگاه آزاد اسلامی، واحد علوم وتحقیقات، تهران، ایران
Mohammad Reza
Fazeli
محمدرضا
فاضلی
fazelimo@tums.ac.ir
100319475328460012548
100319475328460012548
No
Professor of Microbiology, Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
استاد میکروبیولوژی، گروه کنترل دارو وغذا، دانشکده داروسازی، دانشگاه علوم پزشکی تهران، ایران
Nasrin
Samadi
نسرین
صمدی
samadin@tums.ac.ir
100319475328460012549
100319475328460012549
Yes
Associate Professor of Pharmaceutics, Department of Drug and Food Control, Faculty of Pharmacy; and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran
دانشیار فارماسیوتیکس، گروه کنترل دارو وغذا، دانشکده داروسازی و مرکز تحقیقات تضمین کیفیت دارو، دانشگاه علوم پزشکی تهران، ایران