[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 28, Issue 2 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(2): 81-91 Back to browse issues page
Protective effects of vitamin E on sperm characteristics and some serum parameters in mice toxicity induced by dianabol
Mohammad Babaei1, Zahra Tootian *2, Hassan Morovvati3, Bahador Shojaei4, Simin Fazelipour5
1- PhD Student, Department of Anatomy & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2- Department of Anatomy & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , ztotian@ut.ac.ir
3- Department of Comparative Histology & Embryology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4- Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
5- Department of Anatomy, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Abstract:   (1096 Views)
Background: Anabolic steroid drugs, especially dianabol, were used as energy producer drug for growth and enhancement of muscles in athletes. Using of anabolic-androgenic steroids among athletes is extended. However, there are limited information about the adverse effects of mentioned drugs on genital system. This study aimed to evaluation of protective effects of vitamin E against damages caused by dianabol on male genital system.
Materials and methods: In this experimental study, 72 male mice grouped randomly to one control group and seven experimental groups (n=9). Mice in experimental groups 1, 5, 6 and 7 received vitamin E with dose 100 IU/kgBW. Experimental groups 5, 6 and 7 were administrated dianabol with doses 5, 10 and 20 mg/kgBW after 4 hours of vitamin E reception. Mice in experimental groups 2, 3 and 4 received just dianabol with doses 5, 10 and 20 mg/kgBW. Administration method in all groups was orally by gavage for 42 days. 24 hours after last treatment, blood samples were collected from heart and sperm characteristics were evaluated.
Results: Results showed significant decrease in total antioxidant capacity (TAC) and increase in malondialdehyde (MDA) in serum; vitamin E administration remarkably improved above mentioned parameters. Also, vitamin E administration caused improvement in sperm parameters and blood testosterone level.
Conclusion: This study showed that vitamin E, as inhibitor of free radical, can decrease oxidative damages caused by dianabol.
Keywords: Vitamin E, Dianabol, Sperm, Testosterone, Mice
Full-Text [PDF 319 kb]   (405 Downloads)    
Semi-pilot: Experimental | Subject: Anatomical Sciences
Received: 2018/01/14 | Accepted: 2018/01/31 | Published: 2018/06/19
1. Hershberger LG, Shipley EG, Meyer RK. Myotrophic activity of 19-nortestosterone and other steroids determined by modified levator ani muscle method. Proceedings of the Society for Experimental Biology and Medicine Society for Experimental Biology and Medicine (New York, NY) 1953;83:175-80. [DOI:10.3181/00379727-83-20301]
2. Wade N. Anabolic Steroids: Doctors Denounce Them, but Athletes Aren't Listening. Science 1972;176:1399-1403. [DOI:10.1126/science.176.4042.1399]
3. Hartgens F, Kuipers H. Effects of androgenic-anabolic steroids in athletes. Sports Med 2004;34:513-54. [DOI:10.2165/00007256-200434080-00003]
4. Clark AS, Harrold EV, Fast AS. Anabolic-androgenic steroid effects on the sexual behavior of intact male rats. Horm Behav 1997;31:35-46. [DOI:10.1006/hbeh.1997.1355]
5. Pope HG Jr, Katz DL. Psychiatric and medical effects of anabolic-androgenic steroid use. A controlled study of 160 athletes. Arch Gen Psychiatry 1994;51:375-82. [DOI:10.1001/archpsyc.1994.03950050035004]
6. Bahrke MS, Yesalis CE, Wright JE. Psychological and behavioural effects of endogenous testosterone and anabolic-androgenic steroids. Sports Med1996;22:367-90. [DOI:10.2165/00007256-199622060-00005]
7. Torres-Calleja J, Gonzalez-Unzaga M, DeCelis-Carrillo R, Calzada-Sanchez L, Pedron N. Effect of androgenic anabolic steroids on sperm quality and serum hormone levels in adult male bodybuilders. Life Sci 2001;68:1769-74. [DOI:10.1016/S0024-3205(01)00972-9]
8. Machado MV, Cortez-Pinto H. The dark side of sports: using steroids may harm your liver. Liver Int 2011;31:280-1. [DOI:10.1111/j.1478-3231.2010.02445.x]
9. Sanchez-Osorio M, Duarte-Rojo A, Martinez-Benitez B, Torre A, Uribe M. Anabolic-androgenic steroids and liver injury. Liver Int 2008;28:278-82. [DOI:10.1111/j.1478-3231.2007.01579.x]
10. Sale GE, Lerner KG. Multiple tumors after androgen therapy. Arch Pathol Lab Med 1977;101:600-3.
11. Velazquez I, Alter BP. Androgens and liver tumors: Fanconi's anemia and non-Fanconi's conditions. Am J Hematol 2004;77:257-67. [DOI:10.1002/ajh.20183]
12. Pomara C, Neri M, Bello S, Fiore C, Riezzo I, Turillazzi E. Neurotoxicity by Synthetic Androgen Steroids: Oxidative Stress, Apoptosis, and Neuropathology: A Review. Curr Neuropharmacol 2015;13:132-45. [DOI:10.2174/1570159X13666141210221434]
13. Al-Attar AM. Antioxidant effect of vitamin E treatment on some heavy metals-induced renal and testicular injuries in male mice. Saudi J Biol Sci 2011;18:63-72. [DOI:10.1016/j.sjbs.2010.10.004]
14. Zingg JM, Azzi A. Non-antioxidant activities of vitamin E. Curr Med Chem 2004;11:1113-33. [DOI:10.2174/0929867043365332]
15. Bramley PM, Elmadfa I, Kafatos A, Kelly FJ, Manios YR, HE SW, et al. vitamin E. J Sci Food Agric 2000;80:913-38. https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<913::AID-JSFA600>3.0.CO;2-3 [DOI:10.1002/(SICI)1097-0010(20000515)80:73.0.CO;2-3]
16. Chandra AK, Chatterjee A, Ghosh R, Sarkar M. Vitamin E-supplementation protect chromium (VI)-induced spermatogenic and steroidogenic disorders in testicular tissues of rats. Food Chem Toxicol 2010;48:972-9. [DOI:10.1016/j.fct.2010.01.008]
17. Latchoumycandane C, Mathur PP. Effects of vitamin E on reactive oxygen species-mediated 2,3,7,8-tetrachlorodi-benzo-p-dioxin toxicity in rat testis. J Appl Toxicol 2002;22:345-51. [DOI:10.1002/jat.866]
18. Yue D, Yan L, Luo H, Xu X, Jin X. Effect of Vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Anim Reprod Sci 2010;118:217-22. [DOI:10.1016/j.anireprosci.2009.08.004]
19. Zarei L, Sadrkhanlou R, Shahrooz R, Malekinejad H, Eilkhanizadeh B, Ahmadi A. Protective effects of vitamin E and Cornus mas fruit extract on methotrexate-induced cytotoxicity in sperms of adult mice. Vet Res Forum 2014;5:21-7.
20. Rezvanfar M, Sadrkhanlou R, Ahmadi A, Shojaei-Sadee H, Rezvanfar M, Mohammadirad A, et al. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Hum Exp Toxicol 2008;27:901-10. [DOI:10.1177/0960327108102046]
21. Rezvanfar MA, Rezvanfar MA, Shahverdi AR, Ahmadi A, Baeeri M, Mohammadirad A, et al. Protection of cisplatin-induced spermatotoxicity, DNA damage and chromatin abnormality by selenium nano-particles. Toxicol Appl Pharmacol 2013;266:356-65. [DOI:10.1016/j.taap.2012.11.025]
22. Bahrke MS, Yesalis CE. Abuse of anabolic androgenic steroids and related substances in sport and exercise. Curr Opin Pharmacol 2004;4:614-20. [DOI:10.1016/j.coph.2004.05.006]
23. Feinberg MJ, Lumia AR, McGinnis MY. The effect of anabolicandrogenic steroids on sexual behavior and reproductive tissues in male rats. Physiol Behav 1997;62:23-30. [DOI:10.1016/S0031-9384(97)00105-4]
24. Takahashi M, Tatsugi Y, Kohno T. Endocrinological and pathological effects of anabolic-androgenic steroid in male rats. Endocr J 2004;51:425-34. [DOI:10.1507/endocrj.51.425]
25. Torres-Calleja J, González-Unzaga M, DeCelis-Carrillo R, Calzada-Sánchez L, Pedrón N. Effect of androgenic anabolic steroids on sperm quality and serum hormone levels in adult male bodybuilders. Life Sci 2001;68:1769-74. [DOI:10.1016/S0024-3205(01)00972-9]
26. Karila T, Hovatta O, Seppala T. Concomitant abuse of anabolic androgenic steroids and human chorionic gonadotrophin impairs spermatogenesis in power athletes. Int J Sports Med 2004;25:257-63. [DOI:10.1055/s-2004-819936]
27. Hess RA, Cooke PS, Hofmann MC, Murphy KM. Mechanistic insights into the regulation of the spermatogonial stem cell niche. Cell Cycle 2006;5:1164-70. [DOI:10.4161/cc.5.11.2775]
28. Mruk DD, Cheng CY. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 2004;25:747-806. [DOI:10.1210/er.2003-0022]
29. Sader MA, Griffiths KA, McCredie RJ, Handelsman DJ, Celermajer DS. Androgenic anabolic steroids and arterial structure and function in male bodybuilders. J Am Coll Cardiol 2001;37:224-30. [DOI:10.1016/S0735-1097(00)01083-4]
30. Nesterin MF, Budik VM, Narodetskaia RV, Solov'eva GI, Stoianova VG. Effect of methandrostenolone on liver morphology and enzymatic activity. Farmakol Toksikol 1980;43:597-601.
31. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol 2008;59:2-11. [DOI:10.1111/j.1600-0897.2007.00559.x]
32. Agarwal A, Sharma RK, Desai NR, Prabakaran S, Tavares A, Sabanegh E. Role of oxidative stress in pathogenesis of varicocele and infertility. Urology 2009;73:461-9. [DOI:10.1016/j.urology.2008.07.053]
33. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79:829-43. [DOI:10.1016/S0015-0282(02)04948-8]
34. Harkness RA, Kilshaw BH, Hobson BM. Effects of large doses of anabolic steroids. Br J Sports Med 1975;9:70-3. [DOI:10.1136/bjsm.9.2.70]
35. Lejeune H, Chuzel F, Thomas T, Avallet O, Habert R, Durand P, et al. [Paracrine regulation of Leydig cells]. Ann Endocrinol (Paris) 1996;57:55-63.
36. Henricks DM, Kouba AJ, Lackey BR, Boone WR, Gray SL. Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol Reprod 1998;59:330-7. [DOI:10.1095/biolreprod59.2.330]
37. Klaiber EL, Henzl MR, Lloyd CW, Segre EJ. Corpus Luteum-Inhibiting Action of Oxymetholone. J Clin Endocrinol Metab 1973;36:142-7. [DOI:10.1210/jcem-36-1-142]
38. Singer TM, Yauk CL. Germ cell mutagens: risk assessment challenges in the 21st century. Environ Mol Mutagen 2010;51:919-28. [DOI:10.1002/em.20613]
39. Shalizar Jalali A, Najafi G, Hosseinchi M, Sedighnia A. Royal Jelly alleviates sperm toxicity and improves in vitro fertilization outcome in Stanozolol-treated mice. Iran J Reprod Med 2015;13:15-22.
40. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003;49:83-94. [DOI:10.1080/01485010390129269]
41. Lucesoli F, Fraga CG. Oxidative stress in testes of rats subjected to chronic iron intoxication and alpha-tocopherol supplementation. Toxicology 1999;132:179-86. [DOI:10.1016/S0300-483X(98)00152-8]
42. Senthil kumar J, Banudevi S, Sharmila M, Murugesan P, Srinivasan N, Balasubramanian K, et al. Effects of Vitamin C and E on PCB (Aroclor 1254) induced oxidative stress, androgen binding protein and lactate in rat Sertoli cells. Reprod Toxicol 2004;19:201-8. [DOI:10.1016/j.reprotox.2004.08.001]
43. Abdel Aziz AH, Shouman SA, Attia AS, Saad SF. A study on the reproductive toxicity of erythrosine in male mice. Pharmacol Res 1997;35:457-62. [DOI:10.1006/phrs.1997.0158]
44. Pena FJ, Johannisson A, Wallgren M, Rodriguez Martinez H. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim Reprod Sci 2003;78:85-98. [DOI:10.1016/S0378-4320(03)00049-6]
45. Suzuki N, Sofikitis N. Protective effects of antioxidants on testicular functions of varicocelized rats. Yonago Acta Medica 1999;42:87-94.
46. Venkatesh S, Gurdeep Singh M, Prasad Gupta N, Kumar R, Deecaraman M, Dada R. Correlation of sperm morphology and oxidative stress in infertile men. Int J Reprod Biomed 2009;7:29-34.
47. Zahmatkesh E, Najafi G, Nejati V, Heidari R. Protective effect of royal jelly on the sperm parameters and testosterone level and lipid peroxidation in adult mice treated with oxymetholone. AJP 2014;4:43-52.
48. Schurmeyer T, Knuth UA, Belkien L, Nieschlag E. Reversible azoospermia induced by the anabolic steroid 19-nortestosterone. Lancet 1984;1:417-20. [DOI:10.1016/S0140-6736(84)91752-5]
49. Agarwal A, Saleh RA. Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 2002;29:817-27. [DOI:10.1016/S0094-0143(02)00081-2]
50. Gomez E, Irvine DS, Aitken RJ. Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4-hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function. Int J Androl 1998;21:81-94. [DOI:10.1046/j.1365-2605.1998.00106.x]
51. Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, et al. Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 2003;49:83-94. [DOI:10.1080/01485010390129269]
52. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996;48:835-50. [DOI:10.1016/S0090-4295(96)00313-5]
53. Pascoe GA, Fariss MW, Olafsdottir K, Reed DJ. A role of vitamin E in protection against cell injury. Maintenance of intracellular glutathione precursors and biosynthesis. Eur J Biochem 1987;166:241-7. [DOI:10.1111/j.1432-1033.1987.tb13508.x]
54. Cassano E, Tosto L, Balestrieri M, Zicarelli L, Abrescia P. Antioxidant defense in the follicular fluid of water buffalo. Cell Physiol Biochem 1999;9:106-16. [DOI:10.1159/000016307]
55. Garrido N, Meseguer M, Simon C, Pellicer A, Remohi J. Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J Androl 2004;6:59-65.
56. Nagai T, Inoue R, Suzuki N, Nagashima T. Antioxidant properties of enzymatic hydrolysates from royal jelly. J Med Food 2006;9:363-7. [DOI:10.1089/jmf.2006.9.363]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Babaei M, Tootian Z, Morovvati H, Shojaei B, Fazelipour S. Protective effects of vitamin E on sperm characteristics and some serum parameters in mice toxicity induced by dianabol . MEDICAL SCIENCES. 2018; 28 (2) :81-91
URL: http://tmuj.iautmu.ac.ir/article-1-1400-en.html

Volume 28, Issue 2 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3877