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:: Volume 30, Issue 3 (Fall 2020) ::
MEDICAL SCIENCES 2020, 30(3): 244-252 Back to browse issues page
Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model
Samireh Nemati1 , Mohammad Amin Edalatmanesh 2
1- MSc in Cell and Developmental Biology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- of Physiology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran , amin.edalatmanesh@gmail.com
Abstract:   (464 Views)
Background: Prenatal Restraint stress (PRS) leads to cognitive-behavioral disorders and neuro-pathological deficits in children. The present study evaluated the effect of trichostatin A (TSA) on hippocampal level of pro- and anti-inflammatory cytokines in rat model of prenatal restraint stress.
Materials and methods: In this experimental study, 20 pregnant Wistar rats were randomly divided into 4 groups, including control, PRS+NS, PRS+TSA5, and PRS+TSA10. PRS was induced from 13 embryonic day, one hour per day during seven days. Two hours before PRS, administration of TSA was intraperitoneally performed. At the end of one month, the hippocampal levels of pro-inflammatory cytokines such as interleukine-1β (IL-1β), interleukine-6 (IL-6),tumor necrosis factor-α (TNF-α) and anti-inflammatory cytokine (interleukine-10; IL-10) were measured by ELISA technique. Statistical differences between groups were analyzed by ANOVA and Tukey post hoc test and p˂0.05 was considered statistically significant.
Results: A significant increase in pro-inflammatory cytokines content and decrease level of IL-10 in hippocampus were observed in PRS+NS group compared to the control (p˂0.05). However, TSA treatment was significantly decreased IL-1β, IL-6 and TNF-α and increased IL-10 compared to the PRS+NS group in dose manner (p˂0.05).
Conclusion: It seems that TSA with anti-inflammatory effects leads to the modification of hippocampal inflammatory cytokines and reduces inflammation in offspring’s hippocampus who exposed to stress in the prenatal period.
Keywords: Restraint stress, Pregnancy, Inflammatory cytokine, Hippocampus, Rat.
Full-Text [PDF 354 kb]   (199 Downloads)    
Semi-pilot: Experimental | Subject: Physiology
Received: 2019/06/22 | Accepted: 2019/09/14 | Published: 2020/09/20
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Nemati S, Edalatmanesh M A. Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model. MEDICAL SCIENCES. 2020; 30 (3) :244-252
URL: http://tmuj.iautmu.ac.ir/article-1-1807-en.html


Volume 30, Issue 3 (Fall 2020) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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