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:: Volume 30, Issue 3 (Fall 2020) ::
MEDICAL SCIENCES 2020, 30(3): 244-252 Back to browse issues page
Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model
Samireh Nemati1 , Mohammad Amin Edalatmanesh 2
1- MSc in Cell and Developmental Biology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2- of Physiology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran , amin.edalatmanesh@gmail.com
Abstract:   (464 Views)
Background: Prenatal Restraint stress (PRS) leads to cognitive-behavioral disorders and neuro-pathological deficits in children. The present study evaluated the effect of trichostatin A (TSA) on hippocampal level of pro- and anti-inflammatory cytokines in rat model of prenatal restraint stress.
Materials and methods: In this experimental study, 20 pregnant Wistar rats were randomly divided into 4 groups, including control, PRS+NS, PRS+TSA5, and PRS+TSA10. PRS was induced from 13 embryonic day, one hour per day during seven days. Two hours before PRS, administration of TSA was intraperitoneally performed. At the end of one month, the hippocampal levels of pro-inflammatory cytokines such as interleukine-1β (IL-1β), interleukine-6 (IL-6),tumor necrosis factor-α (TNF-α) and anti-inflammatory cytokine (interleukine-10; IL-10) were measured by ELISA technique. Statistical differences between groups were analyzed by ANOVA and Tukey post hoc test and p˂0.05 was considered statistically significant.
Results: A significant increase in pro-inflammatory cytokines content and decrease level of IL-10 in hippocampus were observed in PRS+NS group compared to the control (p˂0.05). However, TSA treatment was significantly decreased IL-1β, IL-6 and TNF-α and increased IL-10 compared to the PRS+NS group in dose manner (p˂0.05).
Conclusion: It seems that TSA with anti-inflammatory effects leads to the modification of hippocampal inflammatory cytokines and reduces inflammation in offspring’s hippocampus who exposed to stress in the prenatal period.
Keywords: Restraint stress, Pregnancy, Inflammatory cytokine, Hippocampus, Rat.
Full-Text [PDF 354 kb]   (199 Downloads)    
Semi-pilot: Experimental | Subject: Physiology
Received: 2019/06/22 | Accepted: 2019/09/14 | Published: 2020/09/20
1. 1. van den Bergh BRH, Dahnke R, Mennes M. Prenatal stress and the developing brain: Risks for neurodevelopmental disorders. Dev Psychopathol 2018; 30:743-62. [DOI:10.1017/S0954579418000342]
2. Soares-Cunha C, Coimbra B, Borges S, Domingues AV, Silva D, Sousa N, et al. Mild Prenatal Stress Causes Emotional and Brain Structural Modifications in Rats of Both Sexes. Front Behav Neurosci 2018; 12:129. [DOI:10.3389/fnbeh.2018.00129]
3. Miranda A, Sousa N. Maternal hormonal milieu influence on fetal brain development. Brain Behav 2018; 8:e00920. [DOI:10.1002/brb3.920]
4. Ortiz JB, Conrad CD. The impact from the aftermath of chronic stress on hippocampal structure and function: Is there a recovery? Front Neuroendocrinol 2018; 49:114-23. [DOI:10.1016/j.yfrne.2018.02.005]
5. McGowan PO, Matthews SG. Prenatal Stress, Glucocorticoids, and Developmental Programming of the Stress Response. Endocrinology 2018; 159:69-82. [DOI:10.1210/en.2017-00896]
6. Fatima M, Srivastav S, Mondal AC. Prenatal stress and depression associated neuronal development in neonates. Int J Dev Neurosci 2017; 60:1-7. [DOI:10.1016/j.ijdevneu.2017.04.001]
7. Glover V. Maternal depression, anxiety and stress during pregnancy and child outcome; what needs to be done. Best Pract Res Clin Obstet Gynaecol 2014;2 8:25-35. doi: 10.1016/j.bpobgyn.2013.08.017. [DOI:10.1016/j.bpobgyn.2013.08.017]
8. Sobolewski M, Conrad K, Marvin E, Allen JL, Cory-Slechta DA. Endocrine active metals, prenatal stress and enhanced neurobehavioral disruption. Horm Behav 2018; 101:36-49. [DOI:10.1016/j.yhbeh.2018.01.004]
9. Weinstock M. Sex-dependent changes induced by prenatal stress in cortical and hippocampal morphology and behaviour in rats: an update. Stress 2011; 14:604-13. [DOI:10.3109/10253890.2011.588294]
10. Calcia MA, Bonsall DR, Bloomfield PS, Selvaraj S, Barichello T, Howes OD. Stress and neuroinflammation: a systematic review of the effects of stress on microglia and the implications for mental illness. Psychopharmacology (Berl) 2016; 233:1637-50. [DOI:10.1007/s00213-016-4218-9]
11. Duthie L, Reynolds RM. Changes in the maternal hypothalamic-pituitary-adrenal axis in pregnancy and postpartum: influences on maternal and fetal outcomes. Neuroendocrinology 2013; 98:106-15. [DOI:10.1159/000354702]
12. Diz-Chaves Y, Pernía O, Carrero P, Garcia-Segura LM. Prenatal stress causes alterations in the morphology of microglia and the inflammatory response of the hippocampus of adult female mice. J Neuroinflammation 2012; 9:71. [DOI:10.1186/1742-2094-9-71]
13. Pearlstein T. Depression during Pregnancy. Best Pract Res Clin Obstet Gynaecol 2015; 29:754-64. [DOI:10.1016/j.bpobgyn.2015.04.004]
14. Pawluski JL, Gemmel M. Perinatal SSRI medications and offspring hippocampal plasticity: interaction with maternal stress and sex. Hormones (Athens) 2018; 17:15-24. [DOI:10.1007/s42000-018-0011-y]
15. Ganai SA, Ramadoss M, Mahadevan V. Histone Deacetylase (HDAC) Inhibitors - emerging roles in neuronal memory, learning, synaptic plasticity and neural regeneration. Curr Neuropharmacol 2016;14:55-71. [DOI:10.2174/1570159X13666151021111609]
16. Sharma S, Taliyan R, Ramagiri S. Histone deacetylase inhibitor, trichostatin A, improves learning and memory in high-fat diet-induced cognitive deficits in mice. J Mol Neurosci 2015; 56:1-11. [DOI:10.1007/s12031-014-0461-x]
17. Zhang Q, Yang F, Li X, Wang L, Chu X, Zhang H, et al. Trichostatin A inhibits inflammation in phorbol myristate acetate induced macrophages by regulating the acetylation of histone and/or non histone proteins. Mol Med Rep 2016; 13:845-52. [DOI:10.3892/mmr.2015.4594]
18. Ma XH, Gao Q, Jia Z, Zhang ZW. Neuroprotective capabilities of TSA against cerebral ischemia/reperfusion injury via PI3K/Akt signaling pathway in rats. Int J Neurosci 2015; 125:140-6. [DOI:10.3109/00207454.2014.912217]
19. Wu J, Song TB, Li YJ, He KS, Ge L, Wang LR. Prenatal restraint stress impairs learning and memory and hippocampal PKCbeta1 expression and translocation in offspring rats. Brain Res 2007;1141:205-13. [DOI:10.1016/j.brainres.2007.01.024]
20. Avila AM, Burnett BG, Taye AA, Gabanella F, Knight MA, Hartenstein P, et al. Trichostatin A increases SMN expression and survival in a mouse model of spinal muscular atrophy. J Clin Invest 2007; 117:659-671. [DOI:10.1172/JCI29562]
21. Porterfield VM, Zimomra ZR, Caldwell EA, Camp RM, Gabella KM, Johnson JD. Rat strain differences in restraint stress induced brain cytokines. Neuroscience 2011; 188: 48-54. [DOI:10.1016/j.neuroscience.2011.05.023]
22. Edalatmanesh MA, Shahsavan S, Rafiei S, Khodabandeh H. The effect of gallic acid on depression symptoms, oxidative stress markers and inflammatory cytokines in rats' hippocampus after TMT intoxication: an experimental study. J Rafsanjan Univ Med Sci 2018; 17: 815-28. [In Persian]
23. Weinstock M. Prenatal stressors in rodents: Effects on behavior. Neurobiol Stress 2017; 6: 3-13. [DOI:10.1016/j.ynstr.2016.08.004]
24. Schepanski S, Buss C, Hanganu-Opatz IL, Arck PC. Prenatal Immune and Endocrine Modulators of Offspring's Brain Development and Cognitive Functions Later in Life. Front Immunol 2018; 9:2186. [DOI:10.3389/fimmu.2018.02186]
25. Su Q, Zhang H, Zhang Y, Zhang H, Ding D, Zeng J, et al. maternal stress in gestation: birth outcomes and stress-related hormone response of the neonates. Pediatr Neonatol 2015; 56:376-81. [DOI:10.1016/j.pedneo.2015.02.002]
26. Myatt L, Thornburg KL. effects of prenatal nutrition and the role of the placenta in health and disease. Methods Mol Biol 2018; 1735:19-46. [DOI:10.1007/978-1-4939-7614-0_2]
27. Gumusoglu SB, Fine RS, Murray SJ, Bittle JL, Stevens HE. The role of IL-6 in neurodevelopment after prenatal stress. Brain Behav Immun 2017; 65:274-283. [DOI:10.1016/j.bbi.2017.05.015]
28. Dahlerup BR, Egsmose EL, Siersma V, Mortensen EL, Hedegaard M, Knudsen LE, et al. Maternal stress and placental function, a study using questionnaires and biomarkers at birth. PLoS One 2018; 13:e0207184. [DOI:10.1371/journal.pone.0207184]
29. Wang B, Jin K. Current perspectives on the link between neuroinflammation and neurogenesis. Metab Brain Dis 2015; 30:355-65. [DOI:10.1007/s11011-014-9523-6]
30. Barrientos RM, Kitt MM, Watkins LR, Maier SF. Neuroinflammation in the normal aging hippocampus. Neuroscience 2015; 309:84-99. [DOI:10.1016/j.neuroscience.2015.03.007]
31. Bittle J, Stevens HE. The role of glucocorticoid, interleukin-1β, and antioxidants in prenatal stress effects on embryonic microglia. J Neuroinflammation 2018; 15:44. [DOI:10.1186/s12974-018-1079-7]
32. Zhang X, Wang Q, Wang Y, Hu J, Jiang H, Cheng W, et al. Duloxetine prevents the effects of prenatal stress on depressive-like and anxiety-like behavior and hippocampal expression of pro-inflammatory cytokines in adult male offspring rats. J Neuroinflammation 2012; 9:71.
33. Zhao Q, Peng C, Wu X, Chen Y, Wang C, You Z. Maternal sleep deprivation inhibits hippocampal neurogenesis associated with inflammatory response in young offspring rats. Neurobiol Dis 2014; 68:57-65. [DOI:10.1016/j.nbd.2014.04.008]
34. Fleiss B, Nilsson MK, Blomgren K, Mallard C. Neuroprotection by the histone deacetylase inhibitor trichostatin A in a model of lipopolysaccharide-sensitised neonatal hypoxic-ischaemic brain injury. J Neuroinflammation 2012; 9:70. [DOI:10.1186/1742-2094-9-70]
35. Hsing CH, Hung SK, Chen YC, Wei TS, Sun DP, Wang JJ, et al. Histone Deacetylase Inhibitor Trichostatin A Ameliorated Endotoxin-Induced Neuroinflammation and Cognitive Dysfunction. Mediators Inflamm 2015; 2015:163140. [DOI:10.1155/2015/163140]
36. Kannan V, Brouwer N, Hanisch UK, Regen T, Eggen BJ, Boddeke HW. Histone deacetylase inhibitors suppress immune activation in primary mouse microglia. J Neurosci Res 2013; 91:1133-42. [DOI:10.1002/jnr.23221]
37. Rubio-Perez JM, Morillas-Ruiz JM. A review: inflammatory process in Alzheimer's disease, role of cytokines. ScientificWorldJournal 2012; 2012:756357. [DOI:10.1100/2012/756357]
38. Shein NA, Shohami E. Histone deacetylase inhibitors as therapeutic agents for acute central nervous system injuries. Mol Med 2011; 17:448-56. [DOI:10.2119/molmed.2011.00038]
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Nemati S, Edalatmanesh M A. Effects of trichostatin A on pre- and anti-inflammatory cytokines of the hippocampus in rats of the prenatally restraint stressed model. MEDICAL SCIENCES. 2020; 30 (3) :244-252
URL: http://tmuj.iautmu.ac.ir/article-1-1807-en.html

Volume 30, Issue 3 (Fall 2020) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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