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:: Volume 32, Issue 2 (summer 2022) ::
MEDICAL SCIENCES 2022, 32(2): 123-131 Back to browse issues page
Effects of magnetic hydroethanolic extract of Nigella sativa and magnesium oxide nanoparticles (MgO) on catalase gene expression in liver of diabetic male rats
Mahboubeh Amini1 , Maryam Ostadsharif 2, Nooshin Naghsh3
1- MSc Student, Department of Cellular and Molecular,Falavarjan Branch Biology, Islamic Azad University, Isfahan, Iran
2- Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran -Department of Medical Biotechnology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran , maryam.ostadsharif@gmail.com
3- Department of Biologoy, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Abstract:   (1263 Views)
Background: Diabetes is a metabolic disorder with chemical, physiological and pathological changes in the liver. Due to the side effects of insulin, the use of herbal medicines such as Nigella sativa with flavonoid compounds and antioxidant properties is considered. In this study, the effect of magnetic hydroethanolic extract of Nigella sativa and MgO nanoparticles separately and simultaneously on the expression of CAT gene in liver tissue of male diabetic rats was investigated.
Materials and methods: 40 male Wistar rats, after induction of diabetes by injection of streptozotomycin (STZ) at a dose of 60 mg / kg, were treated by magnetic hydroathanolic extract of Nigella sativa at a dose of 250 mg / kg, MgO nanoparticles at a dose of 10 mg / kg, combined magnetic hydroathanolic extract 125 mg / kg and 5 mg / kg MgO nanoparticles, and the extract loaded with MgO nanoparticles every other day for 28 days and the expression of liver CAT gene was evaluated.
Results: The extract loaded with MgO nanoparticles was able to increase CAT gene expression in diabetics to the extent of gene expression in metformin-treated diabetics.
Conclusion: It seems that thymoquinone (TQ) in Nigella sativa due to its antioxidant properties increases the expression of CAT gene and overcomes the oxidative stress caused by diabetes with its protective effect on pancreatic beta cells. Loading the nanoparticles probably resulted in better absorption of the antioxidant compounds in the cell, and the MgO nanoparticles as nanocontractors facilitated the cell's access to the particulate compounds.
 
Keywords: Diabetes, Nigella sativa magnetic hydroethanolic extract, CAT gene, MgO nanoparticles
Full-Text [PDF 830 kb]   (440 Downloads)    
Semi-pilot: Basic | Subject: Biology
Received: 2021/11/3 | Accepted: 2021/12/30 | Published: 2022/07/1
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Amini M, Ostadsharif M, Naghsh N. Effects of magnetic hydroethanolic extract of Nigella sativa and magnesium oxide nanoparticles (MgO) on catalase gene expression in liver of diabetic male rats. MEDICAL SCIENCES 2022; 32 (2) :123-131
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Volume 32, Issue 2 (summer 2022) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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