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:: Volume 34, Issue 2 (summer 2024) ::
MEDICAL SCIENCES 2024, 34(2): 163-169 Back to browse issues page
New Delhi metallo-beta-lactamase-producing Enterobacteriaceae isolated from urine sample in Karaj
Sogol Neysari Tabrizi1 , Azam Haddadi 2, Ebrahim Babapour3
1- MSc, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Assistant professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , haddadi.azam@gmail.com
3- Assistant professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (452 Views)
Background: Urinary tract infection is one of the most important infectious diseases. Beta-lactam antibiotics, especially the third and fourth generations of cephalosporins, are effective in treating this infection. The production of beta-lactamase enzyme by bacteria is the most important mechanism of resistance to various beta-lactam antibiotics, which poses a serious threat to the use of these drugs in the future. The aim of this study is to investigate the presence of new beta-lactamases in Enterobacteriaceae isolated from urinary tract infections.
Materials and methods: In this cross-sectional descriptive research, 100 isolates of Enterobacteriaceae were isolated from urine samples collected from the hospitals and laboratories of Alborz province. The isolates were identified by standard biochemical and microbial tests. The pattern of antibiotic resistance of the isolates to 11 different antibiotics was investigated by the Kirby Bauer method. The isolates were analyzed phenotypically in terms of β-lactamase production (NDM-1). In the case of isolates with β-lactamase blaNDM-1, PCR was done with the help of specific primers, and finally the PCR product was sequenced and analyzed.
Results: The pattern of antibiotic resistance was observed as follows: ampicillin (85%), imipenem (70%), kanamycin (66%), cefotaxime (51%), ceftriaxone (51%), tetracycline (50%), ceftazidime (% 49), ciprofloxacin (46%), neurofloxacin (36%), gentamicin (29%), meropenem (27%). The results of PCR showed that 2% of the isolates had blaNDM-1 gene.
Conclusion: The increase of antibiotic resistance in clinical isolates and the emergence of new beta-lactamases warn that the policy of using antibiotics to treat bacterial infections should be changed.
 
Keywords: Urinary tract infection, Antibiotic resistance, NMD-1, PCR.
Full-Text [PDF 434 kb]   (199 Downloads)    
Semi-pilot: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2023/09/20 | Accepted: 2023/11/12 | Published: 2024/06/30
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Neysari Tabrizi S, Haddadi A, Babapour E. New Delhi metallo-beta-lactamase-producing Enterobacteriaceae isolated from urine sample in Karaj. MEDICAL SCIENCES 2024; 34 (2) :163-169
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Volume 34, Issue 2 (summer 2024) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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