Background: Acceptance, learning and use of preventive health behaviors by teams of health workers, using new techniques, is a necessity and powerful need to improve the quality and efficiency of education. This study aimed to measure the satisfaction of employees from a variety of web-based instruction in preventive intervention for hepatitis B grew.

Materials and methods: This study was done in 2013 on the staff of the health care center of East Tehran. The web-based training were studied after the first test (for 5 sessions in 4 groups of 30), and six months after physical education (7 times in 6 groups of 20 training) by 2 teachers .At the end, questionnaire was completed to assess their satisfaction with the quality, content, presentation, and collaboration on the line. Data were analyzed using software SPSS₁₉ by χ², Pearson's correlation, Fridman and Mann-Whitney U tests.

Results: WBL study showed the highest satisfaction. Frequency of satisfaction were 48.33% for presentation material, 42.5% for group participation, 41.66% for getting personal information bank approval, 39.17% for the finished questionnaire under web page, 48.33% for the working group of instructor and 35% for the meetings of the LMS fit the needs of employees. Demographic variables, except gender of staff members, had effect on satisfaction in the E-learning courses. Overall, the mean knowledge, attitude and behavior before and after WBL training were significantly different (P <0.05).

Conclusion: The results showed that acceptance, learning and teaching practices via WBL is in advance, because staff training programs doesn’t need to leave the service, waste of time, and stress from traffic, and get other sources and content in right time. So it is required to set some measures, such as forming committees, to set priorities by providing a summary web-based learning environment in topics courses.

Keywords: Satisfaction, Web-based teaching, learning, Preventive health behaviors, Universal standard precaution.