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Molecular survey of the frequency of sea and seb genes in Staphylococcus aureus isolated from skin infections in Razi hospital of Tehran
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Katayoon Abolghasemi1   , Naser Harzandi 2, Mehrooz Dezfulian3 |
1- MSc Student of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , Naser.harzandi@kiau.ac.ir
3- Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran |
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Abstract: (4506 Views) |
| Background: Staphylococcus aureus is one of the most common nosocomial and community acquired pathogens. The bacterium has different virulence factors and provides aggressive conditions to the host with secretion of the toxins such as super antigenic enterotoxins. Staphylococcal enterotoxin A and B (SEA,SEB) have the most severe toxic effect among these toxins. The aim of this study was to determine the frequency of A and B enterotoxin genes in Staphylococcus aureus isolated from skin infections in Tehran Razi hospital.
Materials and methods: A total of 65 isolates of Staphylococcus aureus from skin samples were collected and confirmed with phenotypic methods and checked by Polymerase Chain Reaction (PCR) using species specific primers. Then the frequency of the sea and seb genes were detected with PCR using specific primers.
Results: The PCR results showed that 86.20 % of Staphylococcus aureus isolates carried the sea gene and the frequency of seb gene in the tested isolates was 15.40 %.
Conclusion: According to the importance of Staphylococcus aureus enterotoxins and their role in the development and exacerbation of the staphylococcal diseases, the presence and expression of the corresponding genes in clinical isolates must be considered in management of the diseases.
Keywords: Staphylococcus aureus, Enterotoxins A and B, Skin infections. |
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| Keywords: Staphylococcus aureus, Enterotoxins A and B, Skin infections. |
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Full-Text [PDF 159 kb]
(2083 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Microbiology
Received: 2017/01/2 | Accepted: 2017/04/10 | Published: 2017/06/20
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