|
Albendazole induces cell death in human colorectal cancer cell line HT-29
|
Elham Hoveizi    1, Tayebeh Mohammadi2 |
1- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran - Stem cells and Transgenic Technology Research Center (STTRC), Shahid Chamran University of Ahvaz, Ahvaz, Iran , e.hoveizi@yahoo.com
2- Department of Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Kermanshah, Iran |
|
|
Abstract: (3900 Views) |
Background: It is a continuing effort to develop new anticancer drugs. It has been reported that albendazole has antiproliferative effects on several tumor cell lines. The aim of this study was to evaluate the killing effect of albendazole on HT-29 human colorectal cancer cells.
Materials and methods: Cultured HT-29 cells were treated with albendazole in 0.1, 1 and 10 µg/ml concentrations for 1, 3 and 5 days. Cellular viability and proliferation were evaluated by MTT and Trypan blue assays at determined days after treatment.
Results: The comparison of cells count and viability in control group and treated groups with albendazole at different concentrations showed significant differences in days 1, 3 and 5.
Conclusion: Albendazole can suppress HT-29 cells proliferation and induces cell death in a dose and time dependent manner.
Keywords: Albendazol, MTT, Cancer, Cell death. |
|
| Keywords: Albendazol, MTT, Cancer, Cell death. |
|
|
Full-Text [PDF 158 kb]
(2431 Downloads)
|
Semi-pilot: Experimental |
Subject:
Molecular Biology
Received: 2018/03/11 | Accepted: 2018/05/7 | Published: 2018/09/22
|
|
|
|
|
|
|
| References |
1. Liang XJ, Chen C, Zhao Y, Wang PC. Circumventing tumor resistance to chemotherapy by nanotechnology. Methods Mol Biol 2010;596:467-88. [ DOI:10.1007/978-1-60761-416-6_21] 2. Martinez-Espinosa R, Arguello-Garcia R, Saavedra E, Ortega-Pierres G. Albendazole induces oxidative stress and DNA damage in the parasitic protozoan Giardia duodenalis. Front Microbiol 2015;6:800. [ DOI:10.3389/fmicb.2015.00800] 3. Arguello-Garcia R, Cruz-Soto M, Gonzalez-Trejo R, Paz-Maldonado LM, Bazan-Tejeda ML, Mendoza-Hernandez G, et al. An antioxidant response is involved in resistance of Giardia duodenalis to albendazole. Front Microbiol 2015;6:286. [ DOI:10.3389/fmicb.2015.00286] 4. Chu SW, Badar S, Morris DL, Pourgholami MH. Potent inhibition of tubulin polymerisation and proliferation of paclitaxel-resistant 1A9PTX22 human ovarian cancer cells by albendazole. Anticancer Res 2009;29:3791-6. 5. Holden-Dye L, Walker RJ. Anthelmintic drugs and nematicides: studies in Caenorhabditis elegans. WormBook 2014:1-29. [ DOI:10.1895/wormbook.1.143.2] 6. Pourgholami MH, Woon L, Almajd R, Akhter J, Bowery P, Morris DL. In vitro and in vivo suppression of growth of hepatocellular carcinoma cells by albendazole. Cancer Lett 2001;165:43-9. [ DOI:10.1016/S0304-3835(01)00382-2] 7. Baltruskeviciene E, Schveigert D, Stankevicius V, Mickys U, Zvirblis T, Bublevic J, et al. Down-regulation of miRNA-148a and miRNA-625-3p in colorectal cancer is associated with tumor budding. BMC Cancer 2017;17:607. [ DOI:10.1186/s12885-017-3575-z] 8. Matsuda T, Okuyama A. The estimates of 5-year colorectal cancer prevalence in adult population in 2012. Jpn J Clin Oncol 2017;47:669-70. [ DOI:10.1093/jjco/hyx089] 9. Cho YC, Nguyen TT, Park SY, Kim K, Kim HS, Jeong HG, et al. Bromopropane Compounds Increase the Stemness of Colorectal Cancer Cells. Int J Mol Sci 2017;18.pii: E1888. [ DOI:10.3390/ijms18091888] 10. Castro LS, Kviecinski MR, Ourique F, Parisotto EB, Grinevicius VM, Correia JF, et al. Albendazole as a promising molecule for tumor control. Redox Biol 2016;10:90-9. [ DOI:10.1016/j.redox.2016.09.013] 11. Oxberry ME, Reynoldson JA, Thompson RC. The binding and distribution of albendazole and its principal metabolites in Giardia duodenalis. J Vet Pharmacol Ther 2000;23:113-20. [ DOI:10.1046/j.1365-2885.2000.00254.x] 12. Oxberry ME, Thompson RC, Reynoldson JA. Evaluation of the effects of albendazole and metronidazole on the ultrastructure of Giardia duodenalis, Trichomonas vaginalis and Spironucleus muris using transmission electron microscopy. Int J Parasitol 1994;24:695-703. [ DOI:10.1016/0020-7519(94)90123-6] 13. Tang Y, Liang J, Wu A, Chen Y, Zhao P, Lin T, et al. Co-Delivery of Trichosanthin and Albendazole by Nano-Self-Assembly for Overcoming Tumor Multidrug-Resistance and Metastasis. ACS Appl Mater Interfaces 2017;9:26648-64. [ DOI:10.1021/acsami.7b05292] 14. Krucken J, Fraundorfer K, Mugisha JC, Ramunke S, Sifft KC, Geus D, et al. Reduced efficacy of albendazole against Ascaris lumbricoides in Rwandan schoolchildren. Int J Parasitol Drugs Drug Resist 2017;7:262-71. [ DOI:10.1016/j.ijpddr.2017.06.001] 15. Zhang X, Zhao J, Gao X, Pei D, Gao C. Anthelmintic drug albendazole arrests human gastric cancer cells at the mitotic phase and induces apoptosis. Exp Ther Med 2017;13:595-603. [ DOI:10.3892/etm.2016.3992] 16. Patel K, Doudican NA, Schiff PB, Orlow SJ. Albendazole sensitizes cancer cells to ionizing radiation. Radiat Oncol 2011;6:160. [ DOI:10.1186/1748-717X-6-160] 17. Michaelis M, Agha B, Rothweiler F, Loschmann N, Voges Y, Mittelbronn M, et al. Identification of flubendazole as potential anti-neuroblastoma compound in a large cell line screen. Sci Rep 2015;5:8202. [ DOI:10.1038/srep08202] |
|
|
