:: Volume 28, Issue 4 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(4): 290-296 Back to browse issues page
Exploring malachite green residues in farmed rainbow trout muscle tissue using high performance liquid chromatography
Sahar Safari1 , Farshad Hashemian 2, Hossein Rastegar3 , Mahnaz Qomi4
1- Graduated, Department of Clinical Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences Islamic Azad University, Tehran, Iran
2- Department of Clinical Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences Islamic Azad University, Tehran, Iran , Hashemian.f@iaups.ac.ir
3- Faculty of Pharmacy, Tehran Medical Sciences Islamic Azad University, Tehran, Iran
4- Faculty of Medicinal Chemistry, Tehran Medical Sciences Islamic Azad University, Tehran, Iran
Abstract:   (3645 Views)
Background: Malachite green exhibiting anti-fungal properties has been used illegally in aquaculture. Due to its teratogenicity, it is necessary to monitor that malachite green is not used in fish farming. The aim of the present study was to measure probable levels of the remaining malachite green in rainbow trout fish in Tehran by high performance liquid chromatography (HPLC).
Materials and methods: The present study was conducted on 42 rainbow trout species with an average weight of 850-1000 g purchased from different parts of Tehran during summer 2017. Fish fillet samples were kept at -20°C until analysis. After extraction, the resulting mixture contained leucomalachite green was then oxidized to malachite green and analyzed by HPLC method.
Results: Out of 42 samples, 3 (7.1%) contaminated cases were reported in the concentration range of 78-89 ppb.
Conclusion: According to our results and other studies, malachite green is used not only in Iran but also in many countries illegally. However, by comparing the results from previous studies, it seems that its consumption in Iran’s fishing industry has been declining. Therefore, it is recommended to use alternative materials as fungicides and promote monitoring of its use in the aquaculture industry by inspection authorities.
Keywords: Malachite green, Rainbow trout fish, High performance liquid chromatography
Full-Text [PDF 154 kb]   (1783 Downloads)    
Semi-pilot: Basic | Subject: Pharmacology
Received: 2018/09/4 | Accepted: 2018/10/7 | Published: 2018/12/23
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