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:: Volume 29, Issue 1 (spring 2019) ::
MEDICAL SCIENCES 2019, 29(1): 64-70 Back to browse issues page
Synergistic effect of silver nanoparticles (AgNPs) and gentamicin against clinical isolates of Pseudomona aeruginosa
Somayeh Esmaili *1, Reza Hosseini Doust2
1- Department of Microbiology, Faculty of Advance Science and Technology Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Microbiology, Faculty of Advanced Science & Technology Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract:   (527 Views)
Background: Pseudomona aeruginosa is one of the most important opportunistic pathogens and is one of the main causes of nosocomial infections. Antibiotic therapy has faced with serious problem due to worldwide antibiotic resistance of this organism. The purpose of this study was to evaluate the anti-bacteial effects of AgNPs on Gentamicin against clinical isolate of P. aeruginosa.
Materials and methods: Sixty strains of P. aeruginosa were isolated from blood, wound, respiratory specimens collected from hospitalized patients within Tehran hospitals. All isolates were first confirmed using standard bacteriologic tests. Standard Kirby- Bauer agar diffusion method was used to evaluate the gentamycin resistance pattern of isolates and also their sensitivity pattern to different concentrations of AgNPs. Finally the effects of different combinations of gentamycin and AgNPs on P. aeruginosa were tested and the results were analyzed by statistical methods.
Results: More than 56% of isolates were resistant to gentamicin, and 13% were mentioned as intermediate according to CLSI guidelines. 30% of isolates were sensitive to this antibiotic. Almost all isolates were sensitive to AgNPs at concentrations of up to 10 μg/ml. The ratio of 50:50 of gentamycin and AgNPs sowed efficacy against all clinical isolates and standard.
Conclusion: we concluded that AGNps enhanced anti-bacterial effects of gentamycin at different ratio of two components. The best effects observed when antibiotic and AgNPs were mixed equally at their MIC level.  We assumed that different size of AgNPs should be tested with antibiotic before final recommendation.
Keywords: Synergic, Antibiotic resistance, P. aeruginosa, Gentamicin, Silver nanoparticles
Full-Text [PDF 217 kb]   (196 Downloads)    
Semi-pilot: Quazi-Experimental | Subject: Microbiology
Received: 2018/02/14 | Accepted: 2018/06/17 | Published: 2019/03/11
References
1. Hosseini Doust R, Saberi M, Hosseini MJ, Mohabati Mobarez A. Surveillance of current antibiotic resistance among clinical isolates S. aureus, E. coli and P. aeroginosa collected from five Iranian cities. JPSH 2012;1:27-38.
2. Nazari Monazam A, Hosseini Doust SR, Mirnejad R. Prevalence Per and VEB beta-lactamase genes among Acinetobacte baumanni isolated from patients in Tehran by PCR. Iran J Med Microbiol 2014;8;28-35.
3. Adabi M, Talebi Taher M, Arbabi L, Afshar M, Fathizadeh S, Minaeian S, et al. Determination of Antibiotic Resistance Pattern of Pseudomonas aeruginosa Strains Isolated from Patients with Burn Wounds. J Ardabil Univ Med Sci 2015;15:66-74.
4. Mahan C, Manuselis G. A textbook of Diagnostic microbiology. London :W.B Sounders compony, 1995.
5. Cristobal LF, Martinez-Castanon GA, Martinez-Castanon RE, Loyola- Rodriguez JP, Patino-Marin N, Reyes-Macias JF, et al. Antibacterial effect of silver nanoparticles against Strptococcus mutans. Mater Lett 2009;63:2603-6. [DOI:10.1016/j.matlet.2009.09.018]
6. Couvreur P, Vauthier C. Nanotechnology: intelligent design to treat complex disease. Pharm Res 2006;23:1417-50. [DOI:10.1007/s11095-006-0284-8] [PMID]
7. Eriksen HM, Iversen BG, Aavitsland P. Prevalence of nosocomial infections in hospitals in Norway, 2002 and 2003. J Hosp Infect 2005;60:40-5. [DOI:10.1016/j.jhin.2004.09.038] [PMID]
8. Benneth JE, Dolin R. Principles and practice of affection disease. London: Churchill Livingston, 1995.
9. Douglas MW, Mulholland K, Denyer V, Gottlieb T. Multi-drug resistant pseudo monas Aeruginofa outbreak and a burn unit. Burns 2001;27:131-5. [DOI:10.1016/S0305-4179(00)00084-X]
10. Mirsalehian A, Feyzabadi M. Nakhjavani FA. Jabal Ameli F, Goli H, Kalantari N. Detection ofVEB-1, OXA-10 and PER-1 genotypes in extended-spectrum beta-lactamase- producing Pseudomonas aeruginosa strains isolated from burn patients. Burns 2010;36:70-4. [DOI:10.1016/j.burns.2009.01.015] [PMID]
11. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16:2346-53. [DOI:10.1088/0957-4484/16/10/059] [PMID]
12. Pitout JD, Sanders CC, Sanders WE Jr. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med 1997;103:51-9. [DOI:10.1016/S0002-9343(97)00044-2]
13. 13 . Rajabpour M, Arabestani MR, Yousefi mashof R, Alikhani MY. MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iran J Med Microbiol 2013;7:18-25.
14. Shahcheraghi F, Nikbin VS, Feizabadi MM. Prevalence of ESBLS Gene among Multidrug-Resistant Isolates of Pseudomonas aeruginosa Isolated from Patients in Tehran. Microbe Drug Resist 2009;15:37-9. [DOI:10.1089/mdr.2009.0880] [PMID]
15. Joklik WK, Willett HP, Amos DB, Wilfert CM, Zinsser H. Zinsser Microbiology, 20th Edition, Appleton and Lange, 1992.
16. Mirzai E, Hosseini Doust R, Mirnejad R, Haghighat S, Rabiei HR. Prevalence of Wide-Spectrum betalactamase genes (KPC, NDM) in clinical isolates of A. baumannii. Tropical Medicine and Infection Disease 2014;19;61-9.
17. Hosseini Doust R, Saberi M, Hosseini MJ, Mohabati Mobarez A. Surveillance of current antibiotic resistance among clinical isolates S. aureus, E. coli and P. aeroginosa collected from five Iranian cities. JPSH 2012;1:27-38.
18. Nazari Monazam A, Hosseini Doust SR, Mirnejad R. Prevalence Per and VEB beta-lactamase genes among Acinetobacte baumanni isolated from patients in Tehran by PCR. Iran J Med Microbiol 2014;8;28-35.
19. Adabi M, Talebi Taher M, Arbabi L, Afshar M, Fathizadeh S, Minaeian S, et al. Determination of Antibiotic Resistance Pattern of Pseudomonas aeruginosa Strains Isolated from Patients with Burn Wounds. J Ardabil Univ Med Sci 2015;15:66-74.
20. Mahan C, Manuselis G. A textbook of Diagnostic microbiology. London :W.B Sounders compony, 1995.
21. Cristobal LF, Martinez-Castanon GA, Martinez-Castanon RE, Loyola- Rodriguez JP, Patino-Marin N, Reyes-Macias JF, et al. Antibacterial effect of silver nanoparticles against Strptococcus mutans. Mater Lett 2009;63:2603-6. [DOI:10.1016/j.matlet.2009.09.018]
22. Couvreur P, Vauthier C. Nanotechnology: intelligent design to treat complex disease. Pharm Res 2006;23:1417-50. [DOI:10.1007/s11095-006-0284-8] [PMID]
23. Eriksen HM, Iversen BG, Aavitsland P. Prevalence of nosocomial infections in hospitals in Norway, 2002 and 2003. J Hosp Infect 2005;60:40-5. [DOI:10.1016/j.jhin.2004.09.038] [PMID]
24. Benneth JE, Dolin R. Principles and practice of affection disease. London: Churchill Livingston, 1995.
25. Douglas MW, Mulholland K, Denyer V, Gottlieb T. Multi-drug resistant pseudo monas Aeruginofa outbreak and a burn unit. Burns 2001;27:131-5. [DOI:10.1016/S0305-4179(00)00084-X]
26. Mirsalehian A, Feyzabadi M. Nakhjavani FA. Jabal Ameli F, Goli H, Kalantari N. Detection ofVEB-1, OXA-10 and PER-1 genotypes in extended-spectrum beta-lactamase- producing Pseudomonas aeruginosa strains isolated from burn patients. Burns 2010;36:70-4. [DOI:10.1016/j.burns.2009.01.015] [PMID]
27. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16:2346-53. [DOI:10.1088/0957-4484/16/10/059] [PMID]
28. Pitout JD, Sanders CC, Sanders WE Jr. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med 1997;103:51-9. [DOI:10.1016/S0002-9343(97)00044-2]
29. 13 . Rajabpour M, Arabestani MR, Yousefi mashof R, Alikhani MY. MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iran J Med Microbiol 2013;7:18-25.
30. Shahcheraghi F, Nikbin VS, Feizabadi MM. Prevalence of ESBLS Gene among Multidrug-Resistant Isolates of Pseudomonas aeruginosa Isolated from Patients in Tehran. Microbe Drug Resist 2009;15:37-9. [DOI:10.1089/mdr.2009.0880] [PMID]
31. Joklik WK, Willett HP, Amos DB, Wilfert CM, Zinsser H. Zinsser Microbiology, 20th Edition, Appleton and Lange, 1992.
32. Mirzai E, Hosseini Doust R, Mirnejad R, Haghighat S, Rabiei HR. Prevalence of Wide-Spectrum betalactamase genes (KPC, NDM) in clinical isolates of A. baumannii. Tropical Medicine and Infection Disease 2014;19;61-9.
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Esmaili S, Hosseini Doust R. Synergistic effect of silver nanoparticles (AgNPs) and gentamicin against clinical isolates of Pseudomona aeruginosa . MEDICAL SCIENCES. 2019; 29 (1) :64-70
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Volume 29, Issue 1 (spring 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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