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:: Volume 29, Issue 2 (Summer 2019) ::
MEDICAL SCIENCES 2019, 29(2): 163-170 Back to browse issues page
Evaluation of antibiotic resistance associated with ophthalmic oqxAB pumps in Klebsiella pneumoniae causing urinary tract infection
Atousa Zomorrodi1 , Mohsen Zargar 2, Jamileh Noroozi3
1- MSc Student in Microbiology, Islamic Azad University, Science and Research Branch, Tehran, Iran
2- Department of Microbiology, Qom Azad University, Qom, Iran , zmohsen2002@yahoo.com
3- Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (3871 Views)
Background: The urinary tract infection is the secondary most common human infection. Colon bacteria, including Klebsiella pneumonia, are the main cause of urinary tract infections. The occurrence of the antibiotic resistance is a major problem in the treatment of infections. Beta- lactamases and efflux pumps constitute the major defense mechanisms of antibiotic resistance of Klebsiella pneumoniae. The aim of present study was to detect oqxA and oqxB genes that encoding oqxAB efflux pumps, in the resistant strains of Klebsiella pneumoniae.
Materials and methods: Among 430 urine samples gathered from Mofoid and Modares Hospitals and Farabi Tajrish Laboratory, 100 strains of Klebsiella pneumoniae has been isolated. Antibiotic sensitivity tests have been accomplished by disc diffusion method based on CLSI standard. The ESBL strains have been identified by Roscoe's compound discs. Finally, the presences of oqxAB efflux pumps have been detected by PCR method through finding the genes encoding these pumps.
Results: Among 100 Klebsiella pneumoniae strains, 43% were ESBL positive. Imipenem, piperacillin tazobactam and morphenem were identified more effective than other antibiotics. The prevalence of oqxA and oqxB genes among resistant isolates was 69.76% and 72.1%, respectively.
Conclusion: Regarding our findings, the urinary tract infections caused by Klebsiella pneumoniae were more prevalent in hospitalized patients. As well as, it was shown a higher percentage of resistance in compare with strains isolated from outpatients. Since the high resistance of Klebsiella pneumoniae strains is very worrying, antibiotics prescribing especially carbapenems need to be strongly supervised.
Keywords: Klebsiella pneumonia, Antibiotic resistance, Efflux pump, Urinary tract infection.
Keywords: Klebsiella pneumonia, Antibiotic resistance, Efflux pump, Urinary tract infection.
Full-Text [PDF 572 kb]   (2054 Downloads)    
Semi-pilot: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2018/09/17 | Accepted: 2019/01/20 | Published: 2019/06/18
References
1. Hashemi A, Fallah F, Taherpour A, Goudarzi H, Erfanimanesh S, Taki E. Evaluation of genetic pattern and determination of oqxA gene expression levels among clinical isolates of Klebsiella Pneumoniae strains. J Mazandaran Univ Med Sci 2014;24:48-61. [In Persian]
2. Roy S, Viswanathan R, Singh AK, Das P, Basu S. Sepsis in neonates due to imipenem resistant Klebsiella pneumoniae producing NDM-1 in India. J Antimicrob Chemother 2011;66:1411-3. [DOI:10.1093/jac/dkr068] [PMID]
3. Tsai YK, Fung CP, Lin JC, Chen JH, Chang FY, Chen TL, et al. Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence. Antimicrob Agents Chemother 2011;55:1485-93. [DOI:10.1128/AAC.01275-10] [PMID] [PMCID]
4. Dwyer PL, O'Reilly M. Recurrent urinary tract infection in the female. Curr Opin Obstet Gynecol 2002;14:537-43. [DOI:10.1097/00001703-200210000-00016] [PMID]
5. Bergus G, Urinary tract infections in pregnancy. In: Yankowitz J, Niebyl JR, editors. Drug therapy in pregnancy. 3rd ed. Philadelphia: Lippincott Williams & Wilkins; 2001. P.63-72.
6. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta-lactamases: the quiet before the storm? Clin Microbiol Rev 2005;18:306-25. [DOI:10.1128/CMR.18.2.306-325.2005] [PMID] [PMCID]
7. Kunin CM, editor. Urinary tract infections. Detection, prevention, and management. Baltimore: Williams & Wilkins, USA; 1997.
8. Fallah F, Hakemi Vala MH, Hashemi A, Fallah F, Rahmati Roodsari R, Taherpour A, et al. Global spread of new delhi metallo-beta-lactamase-1 (NDM-1). Arch Clin Infect Dis 2012;6:171-7.
9. Rahmati Roodsari R, Taherpour A, Hakemi M, Hashemi A. Carbapenem-resistant bacteria and laboratory detection methods. Arch Pediatr Infect Dis 2013;1:188-91. [DOI:10.5812/pedinfect.5193]
10. Ruiz E, Sáenz Y, Zarazaga M, Rocha-Gracia R, Martínez-Martínez L, Arlet G, et al. qnr, aac (6')-Ib-cr and qepA genes in Escherichia coli and Klebsiella spp.: genetic environments and plasmid and chromosomal location. J Antimicrob Chemother 2012;4:886-89. [DOI:10.1093/jac/dkr548] [PMID]
11. Sanath K, Varela MF. Biochemistry of bacterial multidrug efflux pumps. Int J Mol Sci 2012;13:4484-95. [DOI:10.3390/ijms13044484] [PMID] [PMCID]
12. Hansen, LH, Jensen LB, Sørensen HI, Sørensen SJ. Substrate specificity of the OqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria. J Antimicrob Chemother 2007;60:145-7. [DOI:10.1093/jac/dkm167] [PMID]
13. Pitout JD, Hamilton N, Church DL, Nordmann P, Poirel L. Development and clinical validation of a molecular diagnostic assay to detect CTX-M-type B-lactamases in Enterobacteriaceae. Clin Microb Infect 2007;13:291-7. [DOI:10.1111/j.1469-0691.2006.01645.x] [PMID]
14. Alcantar-Curiel D, Tinoco JC, Gayosso C, Carlos A, Daza C, Perez-Prado MC, et al. Nosocomial bacteremia and urinary tract infections caused by extended-spectrum beta-lactamases producing klebsiella pneumoniae with plasmid carrying bith SHV-5 and TLA-1 genes . Clin Infect Dis 2004;38:1067-74. [DOI:10.1086/382354] [PMID]
15. Pages JM, Lavigne JP, Leflon-Guibout V, Marcon E, Bert F, Noussair L, et al. Efflux pump, the masked side of ß-Lactam resistance in Klebsiella pneumoniae clinical isolates. PLoS One 2009;4:e4817-8. [DOI:10.1371/journal.pone.0004817] [PMID] [PMCID]
16. Kumar V, Sun P, Vamathevan J, Li Y, Ingraham K, Palmer L, et al. Comparative genomics of Klebsiella pneumoniae strains with different antibiotic resistance profiles. Antimicrob Agents Chemother 2011;55:4267-76. [DOI:10.1128/AAC.00052-11] [PMID] [PMCID]
17. Doménech-Sánchez A, Hernández-Allés S, Martínez-Martínez L, Benedí VJ, Albertí S. Identification and characterization of a new porin gene of Klebsiella pneumoniae: its role in b-lactam antibiotic resistance. J Bacteriol 1999;181:2726-32.
18. Umeh EU, Hassan M, Onekutu A. Host- related risk factors associated with asymptomatic bacteriuria in a rural community in Benue State, Nigeria. Nig J Microbiol 2006;20:873-79.
19. Jalalpoor S. Antibiotic resistant pattern in ESBLs producer Klebsiella pneumoniae strains isolated of hospitalized and out patients acquired urinary tract infection. Journal of Isfahan Medical School 2011;142:695-706. [In Persian]
20. Rodríguez-Martínez JM, Díaz de Alba P, Briales A, Machuca J, Lossa M, Fernández-Cuenca F, et al. Contibution of oqxAB efflux pumps, to quinolone resistance in extended-spectrum- B-Lactamase-producing Klebsiella pneumonia. J Antimicrob chemother 2013;68:68-73. [DOI:10.1093/jac/dks377] [PMID]
21. Feizabadi MM, Delfani S, Raji N, Majnooni A, Aligholi M, Shahcheraghi F, et al. Distribution of bla(TEM), bla(SHV), bla(CTX-M) genes among clinical isolates of Klebsiella pneumoniae at Labbafinejad Hospital, Tehran, Iran. Microb Drug Resist 2010;16:49-53. [DOI:10.1089/mdr.2009.0096] [PMID]
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Zomorrodi A, Zargar M, Noroozi J. Evaluation of antibiotic resistance associated with ophthalmic oqxAB pumps in Klebsiella pneumoniae causing urinary tract infection . MEDICAL SCIENCES 2019; 29 (2) :163-170
URL: http://tmuj.iautmu.ac.ir/article-1-1583-en.html


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Volume 29, Issue 2 (Summer 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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