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Study of cloned gene-related protein Vp2 of infectious bursal disease virus- induced apoptosis in human lymphoma B cells in vitro
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Mehrdad HASHEMI   1, Seyed Ali GHarashi , Kazaem Parivar , Abdolkhalegh Dizaji , Mehdi Shamsara , Yousof Doustar , Masoumeh Bakhshayesh |
| 1- , Hashemi_mehrdad@yahoo.com |
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Abstract: (30429 Views) |
Background: Apoptosis or programmed cell death (PCD) is an important mechanism in both development and homeostasis in adult tissue for the removal of superfluous cells, while its induction is an effective therapeutic approach for cancer. The aim of the present study was to evaluate the effect of cloned gene-related protein Vp2 of infectious bursal disease virus in human lymphoma B cells.
Material and methods: In present study after cloning the Vp2 gene in Pichia pastoris system, the Vp2 protein was expressed. Cellular vital capacity was determined by MTT. Then effect of Vp2 protein in human lymphoma B cells was examined by Hoechst staining and flowcytometry techniques, respectively.
Results: During MTT, human lymphoma B cell lines revealed to have a meaningful apoptosis at 1mg and 5mg protein concentrations when compared with controls (p<0.01). Apoptotic bodies appeared by Hoechst staining apoptosis was induced suitably after 48 hours by flowcytometry assay.
Conclusion: The present study is the first study that has revealed the gene-related protein Vp2 induced apoptosis in human lymphoma B cells in vitro. |
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| Keywords: Cloning, Apoptos, VP2 gene, B cell lymphoma. |
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Full-Text [PDF 417 kb]
(3610 Downloads)
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Subject:
Epidemiology
Received: 2010/09/21 | Published: 2006/12/15
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