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NOD2 novel mutations in Iranian Crohn's patients
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Alma Farnood   1, Nosratollah Naderi2 , Manijeh Habibi2 , Hedieh Balaie2 , Homayoun Zojaji2 , Farzad Firouzi2 , Mohsen Chiani2 , Faramarz Derakhshan2 , Rahim Aghazadeh2 , Naser Ebrahimi Daryani3 |
1- Imam Khomeini Hospital, Tehran Medical University, Tehran, Iran. , a_farnood@yahoo.com
2- Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University, M.C., Tehran, Iran.
3- Department of Gastroenterology, Tehran University of Medical Sciences, Tehran, Iran. |
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Abstract: (24240 Views) |
Background: Despite the reported role of three common mutations of the CARD15/NOD2 gene including R702W, G908R and 1007fs in Crohn’s disease (CD), only about 30% of Iranian CD patients carry one of these three variants (R702W). The aim of this study was to screen the hot points of NOD2 gene to find any novel sequence variations in Iranian patients with CD.
Materials and methods: Eighty non-related Crohn's patients from Iranian origin, referred to a tertiary center in a three-year period (2006-2009), were enrolled in this study. The hot points of NOD2 gene (including exons 4 and 8) were evaluated by direct sequencing after amplification of related sequences with polymerase chain reaction (PCR).
Results: A total of 17 sequence variations were identified among these exons of NOD2 gene including 7 novel ones. Three of these new mutations had an allele frequency more than 5%. All new mutations were a consequence of a single nucleotide change, 4 resulted in an aminoacid change while one formed a stop coden. No deletion or insertion mutation was observed in this part of the gene.
Conclusion: This study demonstrated the existence of uncommon NOD2 variants in Iranian patients with CD. It is possible that these mutations play a role in susceptibility to CD in Iranian population. |
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| Keywords: NOD2, Crohn’s, Mutation |
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Subject:
Genetic
Received: 2011/02/12 | Accepted: 2013/05/28 | Published: 2013/05/28
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