[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 21, Issue 3 (fall 2011) ::
MEDICAL SCIENCES 2011, 21(3): 188-195 Back to browse issues page
Evaluation of MYCN amplification in Iranian patients with neuroblastoma by conventional and real time PCR
Behzad Poopak 1, Mohammad Taghi Arzanian2 , Parvaneh Vosough3 , Atoosa Gharib2 , Amir Ali Hamidieh3 , Mohammad Ehsani3 , Masood Parsania4 , Abolfazl Yousefian5 , Mohammad Ali Jahangirpour5 , Kobra Farahani5
1- Islamic Azad University, Tehran Medical Branch, Tehran, Iran , bpoopak@yahoo.com
2- Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Tehran University of Medical Sciences, Tehran, Iran
4- Islamic Azad University, Tehran Medical Branch, Tehran, Iran
5- Payvand Specialty Laboratory, Tehran, Iran
Abstract:   (21955 Views)
Background: There is an international consensus that evaluation of MYCNamplification should be done in all cases of newly diagnosed neuroblastoma. MYCN is the most important prognostic factor for neuroblastoma and determines treatment strategy.
Materials and methods: In this study we evaluated paraffin embedded tissue block and bone marrow aspiration of 75 neuroblastoma patients with mean age of 4.1 years, including 32 female and 43 male, by conventional and also real time quantitative PCR.
Results: Forty eight and 43 percent were MYCN amplification positive by Real time and conventional PCR, respectively. 28% of patients less than one year old and 48% of patients older than one year showed MYCN amplification.50 percent of cases with pathological diagnosis of small round cell tumor had MYCN amplification.
Conclusion: PCR is a fast, reliable and cost effective method for the evaluation of MYCN amplification and can be performed using DNA extracted from small tissue samples and paraffin embedded blocks. As expected regarding detection power and convenience, Real time PCR was superior to conventional PCR.
Keywords: MYCN, Amplification, PCR, Neuroblastoma
Full-Text [PDF 207 kb]   (7019 Downloads)    
Semi-pilot: Experimental | Subject: Labratory Sciences
Received: 2011/11/27 | Accepted: 2013/06/30 | Published: 2013/06/30
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Poopak B, Arzanian M T, Vosough P, Gharib A, Hamidieh A A, Ehsani M, et al . Evaluation of MYCN amplification in Iranian patients with neuroblastoma by conventional and real time PCR. MEDICAL SCIENCES 2011; 21 (3) :188-195
URL: http://tmuj.iautmu.ac.ir/article-1-497-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 3 (fall 2011) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645