Background: According to evidence of therapeutic effects of Komboucha, this study was conducted to investigate the effects of Kombucha topical application Synchronized with Kombucha tea oral administration on third-degree burn wounds healing in Wistar rats. Materials and Methods: In this experimental study, forty Wistar male rats weighing 150-200g were subjected to the deep-burn wounds under general anesthesia in the sterile condition. They were randomly divided into four groups including control (the group without any treatment), sham (sweetened tea treated group), experimental1 (KT treated group) and experimental2 (the group that treated with KT oral administration and homogeneous Kombucha symbiont topical application). After 21-day treatment, the rats were sacrificed using an overdose of anesthetic. Macroscopic and microscopic characteristics of wound were studied.
 Results: Macroscopic evaluation revealed completely closure of burn wounds in experimental groups. Histological studies showed that skin, keratinocyte layer, epidermis and hypodermis thicknesses in the experimental 1 and experimental 2 groups and also number and diameter of hair follicles in the experimental 1 group were significantly increased compared with the control group (P<0.001).
Conclusion: Synchronized oral administration and topical application of Kombucha probably lead to healing acceleration and scar reduction of full-thickness burn wounds.