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:: Volume 22, Issue 4 (winter 2013) ::
MEDICAL SCIENCES 2013, 22(4): 251-258 Back to browse issues page
Effect of insulin–like growth factor-I on apoptosis in Chinese hamster ovary cells for biopharmaceutical production
Somayeh Piroozmand1 , Mehran Miroliaei2 , Seyed mohammad Atyabi 3, Homan Kaghazian4 , Jamal Moshtagheian2 , Shiva Irani5 , Darush Norouzeyan
1- Research and Development Department, Research and Production Complex, Pasteur Institute of Iran, Tehran, IranCell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
2- Cell and Molecular Biology Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
3- Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, Iran , mohammadatyabi@yahoo.com
4- Research and Development Department, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran
5- Research Center of Biotechnology Engineering, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
Abstract:   (15063 Views)
Background: The ability to reducing death due to apoptosis and maintaining extensive levels on cell viability under serum free media in cell culture are important subjects in production of recombinant proteins. Insulin-like growth factor-I (IGF-I), is the growth factor of choice for mammalian cell proliferation in serum-free culture. In addition to its mitogenic activity, it has antiapoptotic activity protecting cultures from diverse death inducing stimuli. In this study, the effect of different concentrations of IGF-I examined on CHO–K1 (Chinese hamster ovary K1 cells) cell line for 24 and 48 hours.
Materials and Methods: In this experimental study, the cell line was cultivated in DMEM supplemented with 10% fetal bovine serum (FBS). Apoptosis process was induced in cells by methotrexate, serum was removed and then 10-50 ng/ml concentrations of IGF-I were added. Apoptosis was assessed by caspase 3 detection kit and cell proliferation and viability determined by MTT assay.
Results: Caspase 3 activity decreased significantly by increasing concentrations of IGF-I. In the highest concentration of IGF-I (50 ng/ml), 1.7 and 1.4 equal decreases of caspase 3 activities, compared with negative control group, were noted after 24 and 48 hours, respectively. It confirmed antiapoptotic activity of growth factor to maintain viability and protect cultures from apoptotic inducing stimuli (methotrexate).
Conclusion: IGF-I, as antiapoptotic factor, decreased programmed death of CHO-K1 cells in apoptotic induced conditions.
Keywords: Apoptosis, Chinese hamster ovary cells, Insulin-like growth factor-I, Caspase 3, Cell culture
Full-Text [PDF 204 kb]   (3582 Downloads)    
Semi-pilot: Experimental | Subject: Molecular Biology
Received: 2012/12/24 | Published: 2013/01/15
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Piroozmand S, Miroliaei M, atyabi S M, Kaghazian H, Moshtagheian J, Irani S et al . Effect of insulin–like growth factor-I on apoptosis in Chinese hamster ovary cells for biopharmaceutical production. MEDICAL SCIENCES 2013; 22 (4) :251-258
URL: http://tmuj.iautmu.ac.ir/article-1-619-en.html


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Volume 22, Issue 4 (winter 2013) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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