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Genetic diversity of the dihydropteroate synthase gene in plasmodium vivax in Hormozgan province
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Ali Haghighi   1, Fatemeh Maghsoodlorad2 , Khojasteh Sharifi sarasiabi3 , Seyyed Javad Seyyed Tabaii4 , Nilofar Taghipour4 , Ehsan Nazemalhosseini Mojarad5 , Nahid Hosseinzadeh4 , Latif Gachkar6 |
1- Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , ahaghighi110@yahoo.com
2- Department of Parasitology and Mycoloy, School of Medicine, Golestan University of Medical Science, Gorgan, Iranof Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- School of Para-Medicine, Hormozgan University of Medical Sciences, Bandar-abbas, Iran
4- Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5- Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6- Infectious Disease and Tropical Medicine Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
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Abstract: (8908 Views) |
Background: Malaria is the most important parasitic disease in the world and in malaria-prone regions is considered as the most serious threat to the human health. The aim of this study was polymorphisms analysis of the dihidropteroate synthetase (dhps) gene in p. vivax in Hormozgan province and to determine the prevalence of mutations in codons 382, 383, 512, 553 and 585 related to sulphadoxine resistance in plasmodium vivax.
Materials and Methods: 118 isolates of P. vivax which had been prepared between 2007 and 2008 to study about dihydrofolate reductase-thymidylate synthase (pvdhfr) gene were also used in this study to analyze genetic diversity of the dihidropteroate synthetase (pvdhp) gene of P. vivax.
Results: Of the 118 blood samples infected with P. vivax, 46 and 72 samples were obtained from Minab and Jask, respectively. No mutant alleles were found in 5 target codons using Nested-PCR and sequencing methods. However one novel mutation in codon 421 in three isolates (2.54%) was revealed.
Conclusion: According to the observed mutation in the gene pvdhfr (data not published), and likely resistance to pyrimethamine, and lack of mutations observed in the pvdhps target codons in this study on the same samples, no resistance to the sulphadoxine "and thus to the Fansidar in P. vivax in Hormozgan province can be concluded. |
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| Keywords: Genetic diversity, Dihidropteroate synthetase gene, Plasmodium vivax, PCR, Hormozgan province. |
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Full-Text [PDF 395 kb]
(2136 Downloads)
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Semi-pilot: Experimental |
Subject:
Genetic
Received: 2014/01/22 | Accepted: 2014/01/22 | Published: 2014/01/22
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