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Effect of Tacrolimus on pyramidal cells of cerebral cortex in experimental brain ischemia model in Wistar rat
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Ghazal Ansarian1   , Shabnam Movassaghi2 , Hamed Shafaroodi3 , Nooshin Panahi Khezri1 , Zahra Nadia Sharifi 4 |
1- Student, Department of Pharmacology and Toxicology, School of Pharmacy, Islamic Azad University, Pharmaceutical Science Branch, Tehran, Iran
2- Department of Anatomy, School of Medicine, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
3- Department of Pharmacology and Toxicology, School of Pharmacy, Islamic Azad University, Pharmaceutical Science Branch, Tehran, Iran
4- Department of Anatomy, School of Medicine, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran , nadiasharifi@yahoo.com |
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Abstract: (8051 Views) |
Background: Cerebral ischemia is known as a main cause of morbidity and mortality in the world and there was no effective treatment yet. Global cerebral ischemia causes loss of pyramidal cells of brain cortex following global ischemic/reperfusion. Recently, using immunophilin ligands has been considered as a potential and appropriate strategy for neuroprotection. Since it was observed that tacrolimus (FK506), a useful immunosuppressant used in organ transplantation, provides neuroprotection and prevents neuronal damage,the importance of immunophilins in the development of neuroprotectors has emerged. In this study, we investigated the neurotrophic effect of the immunosuppressant agent FK506 in rat after global cerebral ischemia.
Materials and methods: In this experimental study, 25 Wistar rats were assigned to control (intact), ischemia and 3 FK506 treated (1,3,6 mg/kg) groups. Both common carotid arteries were occluded for 20 minutes followed by reperfusion. In 3 experimental groups, tacrolimus or FK506 was given as a single dose exactly at the time of reperfusion respectively as 1, 3, 6 mg/kg by intravenous administration (IV). The same doses repeated by intraperitoneally administration (IP) 48 hours after reperfusion. After 4 days, the rats were sacrificed and brain sections were stained by H & E and Nissl.
Results: Our findings showed that 20 min ischemia decreased the number of the cortex pyramidal cells. But there were significant differences between number of cortex pyramidal cells in ischemia and FK506 (6mg/kg) groups.
Conclusion: Our study suggests that tacrolimus has a neurotrophic effect on pyramidal cells of brain cortex and may candidate for treatment of ischemia brain damage. |
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| Keywords: Tacrolimus, Brain cortex, Pyramidal cells, Ischemia |
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Full-Text [PDF 860 kb]
(2675 Downloads)
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Semi-pilot: Experimental |
Subject:
Pharmacology
Received: 2014/06/21 | Accepted: 2014/06/21 | Published: 2014/06/21
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