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Determination of antigenic determinants in the C-terminal 311 amino acids of Haps adhesion from Nontypeable Haemophilus influenza
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Akram Tabatabaee1   , Seyed Davar Siadat2 , Seyed Fazllolah Mousavi3 , Mohammad Reza Aghasadeghi4 |
1- PhD of Microbiology, Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran , akram_tabatabaee@yahoo.com
2- PhD of Medical Microbiology,Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
3- PhD of Microbiology & Immunology, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
4- PhD of Biologic Process,Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran |
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Abstract: (14487 Views) |
Background: Haps protein plays central role in initial interaction of nontypeable Haemophilus influenzae (NTHi) with human respiratory epithelial cells. While other surface-exposed proteins of NTHi are highly variable, The HapS domain is highly conserved among H. influenzae strains. Recent studies demonstrated that HapS adhesive activity resides within the C-terminal 311 amino acids of the protein and also they showed that the C-terminal 311 amino acids of HapS (C-Haps) are capable of eliciting a protective immune response against NTHi colonization.
Materials and methods: The pET24a-chaps plasmid harboring c-haps sequence from NTHi PTCC1766 was constructed. The amino acid sequences of C-Haps of this study was aligned with C-Haps of three NTHi strains (N187, TN106, P860295) and antigenicity plot of studied rC-Haps was done bioinformatic software. The pET24a-chaps expression was conducted in E.coli BL21 (D3E) and its expression was confirmed by SDS-PAGE and Western blotting methods. The rC-Haps was purified via immobilized metal affinity chromatography.
Results: Amino acid sequence alignment of rCHaps sequence of current study and rC-Haps from the NTHi strains N187, TN106, P860295 showed more than %97 identity. Antigenicity plot identified 9 common highly antigenic domains that were located exactly in conserved regions among 4 different NTHi strains.
Conclusion: Due to presence of highly conserved antigenic epitopes among C-Haps of NTHi PTCC1766 and other NTHi strains, rC-Haps of current study could be theoretically a vaccine candidate against NTHi strains of different geographical areas. |
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| Keywords: Nontypeable Haemophilus influenzae, Antigenic epitopes, C-Haps |
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Full-Text [PDF 444 kb]
(5249 Downloads)
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Semi-pilot: Experimental |
Subject:
Microbiology
Received: 2014/06/21 | Accepted: 2014/06/21 | Published: 2014/06/21
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