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Investigation of genes responsible for vancomycin resistance by Multiplex-PCR among enterococci isolated strains from inpatients and outpatients
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Seyed Reza Moaddab1   , Behzad Kazemi Haki 2, Nader Ebrahimi AtashKhosroo3 |
1- TB and Pulmonary Diseases, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran
2- Bachelor of Anesthesia, Tabriz University of Medical Sciences, Tabriz, Iran , Behzad_emt@yahoo.com
3- MSc of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran |
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Abstract: (6744 Views) |
Background: Enterococci have been recognized as clinically important pathogens. Among more than one dozen species of Enterococcus species, Enterococcus faecalis and Enterococcus faecium are major pathogens. Vancomycin resistant Enterococci (VRE) strains have been spread worldwide among inpatients and outpatients. This study was designed to study phenotypic and genotypic characteristics of VRE isolates from inpatients and outpatients.
Materials and methods: Of 193 enterococci isolates, 178 strains were identified as E. faecalis and as 15 E.faecium . Out of 193 isolates strains of enterococci, 22 were detected as VRE. All of these strains were isolated from fecal (6 starins) or clinical samples (16) of hospitalized patients.
Results: The PCR revealed VanA genotype in 10 E. faecium and 2 E. faecalis strains, whereas VanB genotype was detected in 5 E.faecium and 5 E.feacalis strains. No VanC genotype was detected among VRE strains.
Conclusion: Due to limited published studies in Iran for VRE strains, we are not able tot compare our results with other studies, but our findings showed that VRE strains were isolated from normal flora and clinical samples, on the other hand the VanA genotype is more than VanB among VRE strains. |
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| Keywords: Enterococcus, VRE, Multiplex PCR, Resistance genes. |
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Full-Text [PDF 226 kb]
(2997 Downloads)
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Semi-pilot: Experimental |
Subject:
Anesthesiology
Received: 2014/12/22 | Accepted: 2014/12/22 | Published: 2014/12/22
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