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Showing 5 results for Salmonella
Sedigheh Mehrabian, Ahmad Majd,
Volume 19, Issue 3 (10-2009)
Abstract
Background: Chemical carcinogens may be produced by metabolic activity of microbes residing in gastrointestinal system. Researches suggest that the consumption of probiotic cultures may decrease the risk of cancer. The aim of this study was to evaluate inhibitory effects of probiotic cultures on mutant and cancerous cells.
Materials and methods: In this experimental study, antimutagenic effects of probiotic cultures, including Bifidobacterium bifidus, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus rhamnosus and Streptococcus thermophillus, were assessed by the Salmonella/ microsome assay upon sodium azid and nitrosamine by Ames test.
Results: There were anticancer and antimutagenic activities of probiotic cultures. Anticarcinogenic effects of probiotic cultures were mostly above 40%, representing their potent anticarcinogenic activities.
Conclusion: This study showed that probiotic cultures have potent anticarcinogenic and antimutagenic activities.
Negar Nayebi, Seyed Ali Ghorashi, Naser Harzandi, Mehdi Shamsara, Bahman Tabarai, Amir Bakhtiari,
Volume 21, Issue 1 (6-2011)
Abstract
Background: Salmonella enterica serovar Enteritidis which involves poultry is transmitted by food. The aim of this survey was to optimize PCR method in order to detect Salmonella enteritidis in poultry products. Materials and methods: In this basic research, 80 samples (40 broiler meat and 40 egg samples) were obtained from distribution centers in Karaj and suburb. Following the DNA extraction of the samples, PCR was optimized and performed using standard strain of Salmonella enteritidis (RTCC1621) as positive control and primers against the flagella coding sequence of Salmonella Enteritidis genome. Results: The analysis of the PCR products by agarose gel electrophoresis indicated the amplification of 250 bp segments in 16 out of 40 (40%) broiler meat and 9 out of 40 (23%) egg samples. The sensitivity of the PCR at the DNA level was found to be 1pg and the specificity of the PCR was determined using 6 other entric Gram negative bacteria and found to be positive only for Salmonella enteritidis. Conclusion: This study confirmed that PCR provides sensitive, specific and rapid approach for detection of Salmonella enteritidis in food samples.
Mohammad Reza Shafaati, Maryam Shafaati, Morteza Khodadoost,
Volume 22, Issue 4 (1-2013)
Abstract
Background: Salmonella, as a common cause of gastrointestinal disease, that transmitted through contaminated food and products causes many cases of illness death annually. Molecular diagnosis of Salmonella by PCR is used for many animal products, but it is employed as a detector (tracer) for the basic agricultural products in some cases. Tomatoes as one of the crops that are used as raw consumption have been known as one of the sources of Salmonella infection.
Materials and Methods: In this experimental study, we used a PCR on specific primers for hilA gene of Salmonella to detect this gene at the surface and within the inoculated tomatoes, in an synthetic method and in a direct way after enrichment.
Results: The results indicate the effective power of Salmonella on the surface and within the inoculated tomatoes with doses of 101 and 102 bacteria per gram of tomato after 6 hours enrichment in BHI medium, respectively.
Conclusion: This study indicates that PCR with primers specific for the hilA is a sensitive and acceptable method for the detection of Salmonella.
Mohammad Reza Najafi, Mehdi Parviz, Kumarss Amini,
Volume 27, Issue 2 (6-2017)
Abstract
Background: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile.
Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for determination of these target genes by the specific oligonucleotides primers.
Results: Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of Salmonella spp., isolates carried cml/tetR and sipB genes, respectively.
Conclusion: According to our results, detection and genotyping of virulence genes and comparison with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial purposes.
Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR
Venus Sadeghi, Kumarss Amini,
Volume 28, Issue 2 (6-2018)
Abstract
Background: Salmonella, as an aerobic and facultative anaerobe bacillary gram-negative bacterium, is pathogen for humans and animals. This bacterium dominates the vertebral gastrointestinal tract, depending on the serotype and host conditions and factors, causes diseases with various symptoms and complications. Today, the development of multiple antibiotic resistance in this bacterium is a major barrier to public health. Integrons can play an important role in creating and expanding this resistance. The aim of this study was to investigate the presence of class I, 2 and 3 integrons in Salmonella typhimurium isolated of clinical samples by Multiplex PCR.
Materials and methods: In this study, 60 isolates of Salmonella typhimurium were collected from Tehran Hospitals and confirmed by biochemical and culture tests. Multiplex-PCR assay was performed to identify int1, int2 and int3 integrons genes.
Results: Of the 60 strains of Salmonella typhimurium, PCR assays detected 85% integron class 1, 45% integron class 2, and 70% integron class III.
Conclusion: The results of this study showed high incontinence in Salmonella typhimurium strains isolated from clinical cases. Identifying these genes can be an important strategy in identifying and responding to antibiotic resistance, since the presence of infrared cells is an indicator of the development and spread of antibiotic resistance.
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