TY - JOUR T1 - Role of extracted pigment from environmental isolated bacteria on antibiotic resistant isolated microorganisms from diabetic patients TT - نقش پیگمان‌های استخراجی از باکتری‌های جدا شده از محیط بر روی میکروارگانیسم‌های مقاوم به آنتی بیوتیک جدا شده از بیماران دیابتیک JF - iau-tmuj JO - iau-tmuj VL - 28 IS - 2 UR - http://tmuj.iautmu.ac.ir/article-1-1402-en.html Y1 - 2018 SP - 104 EP - 116 KW - Antibiotic susceptibility KW - Bacterial infection KW - Bacterial pigment KW - Diabetes mellitus KW - Thin-layer chromatography. N2 - Background: Diabetes is one of the most important metabolic diseases in the world that can ultimately lead to ulceration and amputation of lower limbs. The aim of this study was pigment extraction from isolated environmental bacteria and their effect on antibiotic resistant bacteria cooperated on infected diabetic foot. Materials and methods: In this project, dominant bacteria were identified using biochemical tests and antibiotic susceptibility was evaluated. Then, effect of different solvent was evaluated on the extraction of pigments using thin layer chromatography. Finally the affected bacteria were molecularly identified. Results: The results obtained from this study indicated that the most common isolated bacteria were Escherichia coli and Staphylococcus aureus. The results from antibiogram tests indicated that most of the gram positive isolates had same results and the gram negative was sensitive to Meropenem and Imipenem. Among extracted pigments, 4 different pigment which is effective on antibiotic resistant bacteria according to molecular technique were Brachybacterium sp. IARI-ABL-35, Brachybacterium nesterenkovii strain DSM 9573, Brevundimonas sp. NeomS2D Kocuria sp. YIM 75764. Conclusion: According to biologic properties of bacterial pigments, we could use them in the treatment of various diseases. M3 10.29252/iau.28.2.104 ER -