TY - JOUR JF - iau-tmuj JO - MEDICAL SCIENCES VL - 28 IS - 2 PY - 2018 Y1 - 2018/6/01 TI - Detection of Human Group A Rotavirus in Urban Sewage, Hospital Sewage and River Water Samples in Alborz Province Using ELISA Method TT - تشخیص روتاویروس انسانی گروه A در نمونه های فاضلاب شهری، فاضلاب بیمارستانی و آب رودخانه در استان البرز به روش الایزا N2 - Background: Human group A rotaviruses are the most common cause of severe diarrhea among children <5 years of age worldwide. Rotaviruses are widely present in environmental water. The aim of this study was to detect the human group A rotaviruses in urban sewage, hospital sewage and river water samples in Alborz province using ELISA method. Materials and methods: This cross-sectional study was carried out on 76 samples collected from both influent and effluent water of 4 sewage treatment systems, hospital sewage, Karaj and Baraghan rivers in Alborz province. All samples were concentrated using pellet method. Afterwards, human group A rotaviruses were detected using ELISA method. Results: In total, rotaviruses were identified in 6 samples (7.89%) using ELISA method. Three positive samples (50%) were related to the raw sewage influent and three positive samples (50%) were related to the Karaj river. The frequency of rotavirus detection in summer, autumn and winter was 1 sample (16.66%), 3 samples (50%) and 2 samples (33.33%), respectively. Conclusion: This study showed the contamination of environmental water by human group A rotaviruses. Therefore, it is necessary to constantly monitor sewage treatment systems and river water for detecting rotaviruses. Keywords: Human group A rotaviruses, Environmental water, Pellet method, ELISA. SP - 124 EP - 129 AU - Torfeh, Zahra AU - Harzandi, Naser AU - Ghaderi, Mostafa AD - Microbiology, Department of Microbiology, Faculty of Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran KW - Human group A rotaviruses KW - Environmental water KW - Pellet method KW - ELISA. UR - http://tmuj.iautmu.ac.ir/article-1-1404-en.html DO - 10.29252/iau.28.2.124 ER -