RT - Journal Article T1 - Exploring malachite green residues in farmed rainbow trout muscle tissue using high performance liquid chromatography JF - iau-tmuj YR - 2018 JO - iau-tmuj VO - 28 IS - 4 UR - http://tmuj.iautmu.ac.ir/article-1-1489-en.html SP - 290 EP - 296 K1 - Malachite green K1 - Rainbow trout fish K1 - High performance liquid chromatography AB - Background: Malachite green exhibiting anti-fungal properties has been used illegally in aquaculture. Due to its teratogenicity, it is necessary to monitor that malachite green is not used in fish farming. The aim of the present study was to measure probable levels of the remaining malachite green in rainbow trout fish in Tehran by high performance liquid chromatography (HPLC). Materials and methods: The present study was conducted on 42 rainbow trout species with an average weight of 850-1000 g purchased from different parts of Tehran during summer 2017. Fish fillet samples were kept at -20°C until analysis. After extraction, the resulting mixture contained leucomalachite green was then oxidized to malachite green and analyzed by HPLC method. Results: Out of 42 samples, 3 (7.1%) contaminated cases were reported in the concentration range of 78-89 ppb. Conclusion: According to our results and other studies, malachite green is used not only in Iran but also in many countries illegally. However, by comparing the results from previous studies, it seems that its consumption in Iran’s fishing industry has been declining. Therefore, it is recommended to use alternative materials as fungicides and promote monitoring of its use in the aquaculture industry by inspection authorities. LA eng UL http://tmuj.iautmu.ac.ir/article-1-1489-en.html M3 10.29252/iau.28.4.290 ER -