fa بررسی اثر باکتری B. thetaiotaomicron و مشتقات آن بر بیان ژن های tlr2,tlr4 در رده سلولیSTC-1 بيماريهاي عفوني Infectious Diseases تجربي Experimental <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">باکتری </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">B. thetaiotaomicron</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> به عنوان کاندید نسل جدید پروبیوتیک ها مطرح </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">است</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">.</span></span></span></i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">TLR2,4</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> در</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">روده نقش مهم و ضروری در شناسایی و فعالسازی سیستم ایمنی ذاتی ، پس از مواجهه با باکتری ها برعهده دارند. هدف از این مطالعه بررسی</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> تاثیر باکتری&nbsp; </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">B. thetaiotaomicron</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> و مشتفات آن برتغییرات بیان ژن های </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">tlr2,tlr4</span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> بود. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">بررسی تاثیر تیمارهای&nbsp; </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">B. thetaiotaomicron</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">، </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">OMVs</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> ، باکتری غیر فعال و سوپرناتانت بر بیان ژنهای </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">tlr2,tlr4</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> در رده سلولی </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">STC-1</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> با استفاده از روش </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">qRT-PCR</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> انجام شد.&nbsp; </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">یافته&nbsp;</span></span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">تیمار رده سلولی</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">STC-1</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> با باکتری زنده و فعال&nbsp; </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">B. thetaiotaomicron</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در تغییر بیان دو ژن </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">tlr2,4</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> تاثیر معنی داری نداشتند. اثر </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">OMVs</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> این باکتری در افزایش بیان دو ژن </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">tlr2</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">tlr4</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در غلظت </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&micro;g/ml</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">50 معنی&shy;دار (01/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، 02/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) بود،</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> ولی در غلظت </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&micro;g/ml </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">100 تغییرات معنی&shy;دار نبود.&nbsp; باکتری غیرفعال در </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MOI 10</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MOI 50</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> تاثیر معنی داری در افزایش دو ژن داشت (به ترتیب 03/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، 003/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). سوپرناتانت 25درصد به صورت قابل توجهی بیان دو ژن </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">tlr2</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> (038/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">tlr4</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> (034/0=</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) را در سطح رونویسی افزایش داد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه&nbsp;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">&shy;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">نتایج این تحقیق نشان داد که </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">OMVs</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> در غلظت 50 میکروگرم برمیلی لیتر، باکتری غیر فعال و سوپر ناتانت </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">B. thetaiotaomicron&nbsp; </span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">&nbsp;&nbsp;نقش مهمی&nbsp; در تقویت سیستم ایمنی و ایمنی زایی ایفا می&shy;کنند و می&shy;توانند به عنوان کاندید نسل جدید پست بیوتیک و پاراپروبیوتیک جهت مطالعات بعدی مورد استفاده قرار بگیرد.</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: <i>B. thetaiotaomicron</i> is introduced as a candidate for the next generation of probiotics. TLR2, 4 play an important and necessary role in activating and modulating the innate immune system after exposure to bacteria in the intestine. This study aimed to investigate the effect of <i>B. thetaiotaomicron</i> and its derivatives on the alteration of the <i>tlr2</i> and <i>tlr4</i> gene expression. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: The effects of <i>B</i>. <i>thetaiotaomicron</i>, OMVs, inactive bacteria and supernatant treatments on the <i>tlr2 </i>and <i>tlr4</i> gene expression in the STC-1 cell line were investigated using the qRT-PCR method. &nbsp;&nbsp;</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: The treatment of the STC-1 cell line with live and active <i>B. thetaiotaomicron</i> did not have significant effect on transcription of&nbsp; <i>tlr2, 4</i>. The OMVs of this bacterium at 50 &micro;g/ml significantly increased the gene expression of <i>tlr2 </i>(<i>p</i>=0.01) and<i> tlr4 </i>(<i>p</i>=0.02), but at a concentration of 100 &micro;g/ml, its effect was not significant. Inactive bacteria at MOI 10 (<i>p</i>=0.03) and MOI 50(<i>p</i>=0.003) significantly induce the transcription of both two genes. Supernatant 25% significantly increased <i>tlr2 (p=0.038) and</i> <i>tlr4 (p=0.034) </i>gene expression at the transcription level.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: Our results showed that OMVs at a concentration of 50 &mu;g/ml, inactive bacteria, and supernatant <i>B. thetaiotaomicron</i> play an important role in modulating immune response and can be used as a next generation postbiotics and paraprobiotic candidates for further studies to be used.</span></span></span></span></span></span><br> &nbsp; باکتروئیدس تتایوتائومیکرون, وزیکول خارج سلولی, میکروبیوتا ,TLR2, TLR4 B.thetaiotaomicron, Microbiota, OMVs, TLR2, TLR4 1 10 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-478-2&slc_lang=fa&sid=1 Somaye Vaezijoze سمیه واعظی جزء somayevaezijoze@gmail.com 100319475328460013293 100319475328460013293 No Department of Biology, Science, and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ، تهران، ایران Shiva Irani شیوا ایرانی s.irani@srbiau.ac.ir 100319475328460013294 100319475328460013294 No Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran گروه زیست شناسی، دانشگاه آزاد واحد علوم و تحقیقات، تهران، ایران Seyed Davar Siadat سید داور سیادت d.siadat@gmail.com 100319475328460013295 100319475328460013295 Yes Department of Mycobacteriology and Pulmonary Research, P7asteur Institute of Iran, Tehran, Iran - Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran بخش سل و تحقیقات ریوی، انستیتو پاستور ایران، تهران، ایران- مرکز تحقیقات میکروب شناسی، انیستیتو پاستور ایران، تهران، ایران Mohammadreza Zali محمدرضا زالی nnzali@hotmail.com 100319475328460013296 100319475328460013296 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran 7 پژوهشکده بیماری های گوارش و کبد، مرکز تحقیقات بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران