Medical Sciences Journal of Islamic Azad University
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی تهران
MEDICAL SCIENCES
Medical Sciences
http://tmuj.iautmu.ac.ir
1
admin
1023-5922
2008-3386
000
10.66224/iau
000
1023-5922
000
fa
jalali
1403
7
1
gregorian
2024
10
1
34
3
online
1
fulltext
fa
طراحی و تهیه نانوذرات کیتوزان-آلژینات حاوی BSA و مطالعه انتشار آن در شرایط in vitro
Design and preparation of Chitosan/Alginate nanoparticles containing BSA and In-vitro release study
داروسازي
Pharmacology
تجربي
Experimental
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">نانوذرات به دلیل ویژگی­های خاصی که دارند، در زمینه سیستم­های واکسن و دارورسانی بیش از پیش مورد توجه قرار گرفته­اند. به طور خاص، آلژینات و کیتوزان به عنوان امیدوار کننده­ترین نانوحامل­ها شناخته شده­اند</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">. هدف مطالعه حاضر، </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">طراحی و تهیه­ نانوذرات کیتوزان-آلژینات حاوی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">BSA</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و مطالعه انتشار آن در شرایط </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> in­-vitro</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">بود.</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">نانوذرات با روش ژل سازی یونی در شرایط ملایم و در محیط آبی تهیه شدند که منجر به حصول ذرات با اندازه و شکل مطلوب، راندمان به دام افتادن بالا و ویژگی های رهایش کنترل شده، شد</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></span><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">­</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">نانوذرات سنتز شده با قطر 60 نانومتر و پتانسیل زتا 32- </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">mv </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">به طور قابل توجهی امیدوارکننده بودند و با روش­های</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> FTIR </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">SEM</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">و</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> DSC</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">ارزیابی شدند. نانوذرات همانند یک پوسته کروی، دارای مورفولوژی سطح صاف و ساختار همگن بودند که توسط تصاویر</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> SEM</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">نشان داده شد. آنالیزهای </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">FTIR</span></span></span></i><i><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">DSC</span></span></span></i><i><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> تغییرات ساختاری را تایید کردند. میزان کارایی احتباس و ظرفیت بارگذاری به ترتیب %92 و %74 به روش غیرمستقیم به دست آمد. مشخصات آزادسازی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">BSA</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> و مکانیسم آن تحت شرایط فیزیولوژیکی شبیه­سازی شده و در شرایط </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">in­-vitro</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> برای دوره­های انکوباسیون مختلف مورد مطالعه قرار گرفت</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> انتشار در ابتدا بسیار سریع و به دنبال آن یک فاز رهاسازی مداوم و کنترل شده را نشان داد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman",serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتیجه</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">نتایج به ما </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">نشان داد که نانوذرات تهیه شده می­توانند برای رسانش </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">BSA</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> مقبول باشند؛ لذا می­توان آن را کاندیدای مناسب برای رسانش داروهای مختلف معرفی کرد.</span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Nanoparticles have received more and more attention in the vaccine and drug delivery systems field due to their specific properties. In particular, alginate and chitosan have been known as the most promising nano-carries. The present study aimed to develop alginate/chitosan nanoparticles as a carrier of BSA to Study In-vitro release condition. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: The nanoparticles were produced by the ionic gelation method in mild conditions and the aqueous environment, which leads to the production of particles with favorable size, shape, high entrapment efficiency, and controlled release characteristics. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: The synthesized nanoparticles of 60 nm in diameter and -32mv in zeta potential were considerably unparalleled and characterized by SEM, FTIR, and DSC. Nanoparticles resembled a spherical shell, smooth morphology, and homogeneous structure shown by SEM images. FTIR and DSC analyses confirmed conformational changes. Entrapment efficiency and loading capacity were 92% and 74%, respectively, via indirect method. The In-vitro BSA release profile and mechanism of BSA release from the nanoparticles were studied under simulated physiological conditions for various incubation periods. An initial burst effect revealed the release profile, followed by a continuous and controlled release phase.</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman",serif"><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: Our results indicated the prepared nanoparticles could be appropriate for the convenient delivery of the desired drugs.</span></span></span></span></span></span><br>
نانوذرات, کیتوزان, آلژینات, BSA, آزادسازی
Nanoparticles, Chitosan, Alginate, BSA, Release.
227
236
http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-1790-1&slc_lang=fa&sid=1
Nazli
Kashani Javid
نازلی
کاشانی جاوید
nazlikjavid@gmail.com
100319475328460012543
100319475328460012543
No
Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
گروه نانو تکنولوژی پزشکی، دانشکده علوم و فناوری های نوین، علوم پزشکی تهران دانشگاه آزاد اسلامی تهران، ایران
Nasser
Mohammadpour
ناصر
محمدپوردونیقی
nasser_mohammadpour@yahoo.com
100319475328460012544
100319475328460012544
Yes
Razi Vaccine and Sera Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران
Sepideh
Arbabi Bidgoli
سپیده
اربابی بیدگلی
sepideharbabi@yahoo.com
100319475328460012545
100319475328460012545
No
Department of Toxicology–Pharmacology, Faculty of Pharmacy, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran
گروه سم شناسی و فارماکولوژی، دانشکده داروسازی و علوم دارویی، علوم پزشکی تهران دانشگاه آزاد اسلامی تهران، ایران