fa بررسی کمی تغییرات بیان ژن T-Bet در بیماران مبتلا به بیماری سلیاک تحت رژیم غذایی فاقد گلوتن زيست شناسي مولكولي Molecular Biology موردي- شاهدي case-control <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">سابقه و اهداف:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> بر اساس مطالعات صورت گرفته، فاکتور رونویسی </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">T-bet</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> می تواند با افزایش پاسخ های با واسطه </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Th1</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> و تولید سایتوکاین های پیش التهابی مانند </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">اینترفرون گاما </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">(IFN&gamma;)</span></span></span> &nbsp;<span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">و فاکتور نکروز دهنده تومور آلفا </span></span></span>&nbsp;<span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">(</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">TNF-&alpha;</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">)</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">بر پیشرفت التهاب و وخیم تر شدن بیماری سلیاک موثر باشد. هدف از انجام این مطالعه بررسی بیان ژن</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">T-bet</span></span></span> &nbsp;<span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">در بیماران مبتلا به بیماری سلیاک تحت رژیم غذایی بدون گلوتن به مدت 6 ماه یا بیشتر د‌‌ر مقایسه با افراد سالم بود. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">روش بررسی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> در این مطالعه ابتدا تعداد 20 نمونه خون محیطی از بیماران مبتلا به بیماری سلیاک و 20 نمونه از افراد سالم به عنوان گروه کنترل جمع آوری شد. پس از استخراج </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">RNA</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> و سنتز </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">cDNA</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> ، جفت پرایمر اختصاصی ژن </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">T-bet</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> طراحی و پس از تایید با نرم افزار بلاست، مورد استفاده قرار گرفت. </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">PCR</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"> انجام گردید و سپس</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">بررسی بیان ژن</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%">T</span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">-bet</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">با روش</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">صورت گرفت</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">یافته ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">در این مطالعه تعداد 12 نفر (60%) زن و 8 نفر (40%) مرد &nbsp;در گروه بیمار (16.07</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:#0d0d0d">&plusmn;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">37.05</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"> (</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">و تعداد 13 نفر (65%) زن و 7 نفر (35%) مرد در گروه کنترل (8.62</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"><span style="color:#0d0d0d">&plusmn;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">36.47</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"> (</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">بررسی شدند. بیان ژن</span></span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">T-</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">bet</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> در بیماران مبتلا به بیماری سلیاک تحت رژیم فاقد گلوتن برای 6 ماه یا بیشتر در مقایسه با افراد سالم اختلاف معنا داری نشان نداد </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">(</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:">0.27</span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">Pvalue:</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">).</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:black">بحث و نتیجه گیری:</span></span></span></span></b> &nbsp;<span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d">نتایج مطالعات حاکی از آن است که افزایش بیان ژن </span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d">T-bet</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" nazanin="" style="font-family:"><span style="color:#0d0d0d"> می تواند در افزایش التهاب در بیماری و پیشرفت بیماری نقش داشته باشد. نتایج بررسی کمّی تغییر بیان این ژن در بیماران مبتلا به بیماری سلیاک تحت درمان با رژیم غذایی فاقد گلوتن (برای6 ماه یا بیشتر) در مقایسه با افراد کنترل نشان داد که نمی توان از این ژن به عنوان بیومارکر تشخیصی جهت افتراق این گروه از بیماران از افراد سالم استفاده نمود.</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:#0d0d0d"></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and Aim:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> Based on previous studies, T-bet can be effective in inflammation and worsening of celiac disease by improving the progression of Th1-mediated responses and the production of pro inflammatory cytokines such as interferon-gamma (IFN&gamma;) and tumor necrosis factor-alpha (TNF-&alpha;). The aim of this study was to evaluate the expression of T-bet gene in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> In this study, 20 peripheral blood samples were collected from patients (37.5&plusmn;16.07) with celiac disease and 20 samples from healthy individuals (36.47&plusmn;8.62) as a control group. After RNA extraction and cDNA synthesis, a specific primer pair of T-bet gene was designed and used after confirmation with blast software. PCR was performed and then T-bet gene expression was assessed by real-time PCR.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> In this study, 12 (60%) females and 8 (40%) males in the patient group and 13 (65%) females and 7 (35%) males in the control group were studied. T-bet gene expression was not significantly different in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals (Pvalue: 0.27).</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion: The results of quantitative analysis of the expression of this gene in patients with celiac disease treated with gluten-free diet (for 6 months or more) compared with controls showed that this gene cannot be used as a diagnostic biomarker to differentiate Patients from healthy individuals.</span></span></span></span></span></span><br> &nbsp; بیماری سلیاک , فاکتور رونویسی T-bet, رژیم فاقد گلوتن, DNA پرایمر,PCR Celiac Disease, T-bet transcription factor, Gluten Free Diet, DNA Primer, PCR 0 0 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-1384-1&slc_lang=fa&sid=1 Fatemeh Ganjali فاطمه گنجعلی 100319475328460011912 100319475328460011912 No Department of Cellular & molecular biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه علوم سلولی و مولکولی، دانشکده علوم و فناوری های نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Mohammad Rostami-Nejad محمد رستمی نژاد 100319475328460011913 100319475328460011913 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Associate Professor, Tehran, Iran مرکز تحقیقات بیماری های گوارش و کبد، پژوهشکده بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، دانشیار، تهران، ایران , Hamid Asadzadeh Aghdaei حمید اسدزاده عقدائی 100319475328460011914 100319475328460011914 No Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Assistant Professor, Tehran, Iran مرکز تحقیقات بیماری های گوارش و کبد، پژوهشکده بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، استادیار، تهران، ایران Mehrdad Hashemi مهرداد هاشمی mhashemi@iautmu.ac.ir 100319475328460011915 100319475328460011915 Yes Farhikhtegan Medical Convergence Science Research Center, Farhikhtegan Hospital Tehran Medical sciences, Islamic Azad University, Tehran, Iran , Department Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Professor, Tehran, Iran مرکز تحقیقات علوم همگرای پزشکی فرهیختگان، بیمارستان فرهیختگان، دانشگاه آزاد اسلامی علوم پزشکی تهران، ایران , گروه ژنتیک، دانشکده علوم و فناوری های نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی، استاد تمام، تهران، ایران