fa بررسی فراوانی DNAویروس سایتومگال در خون افراد HIV مثبت ويروس شناسي Virology تحليلي/مقطعي/توصيفي Survey/Cross Sectional/Descriptive <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-weight:bold"><v:line from="3.6pt,2.8pt" id="_x0000_s1026" strokeweight="1.5pt" style="position:absolute; left:0; text-align:left; z-index:251658240" to="480.6pt,2.8pt"> <w:wrap anchorx="page"> </w:wrap></v:line><br> <span lang="FA" style="font-size:14.0pt"><span style="font-family:&quot;B Nazanin&quot;">چکیده</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal">ویروس </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">HIV</span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal">به عنوان یکی از مهم‌ترین ویروس‌های بیماری‌زا در جوامع بشری شناخته شده است که به علت ایجاد نقص ایمنی، باعث بروز انواع عفونت‌های فرصت‌طلب در بیماران می‌گردد. از مهم‌ترین ویروس‌های فرصت طلب در این افراد، ویروس </span></span></span></i><a name="_Hlk162194353"><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">CMV</span></span></i></a><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal">است که موجب آسیب جدی و کاهش طول عمر آنها می&shy;گردد. به همین&nbsp; علت تشخیص سریع </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">CMV</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal"> در افراد </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">HIV</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal"> مثبت از اهمیت ویژه‌ای برخوردار است. در این پژوهش نشان داده شد که با یک تست ساده در افراد </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">HIV</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal"> مثبت، می‌توان حضور </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">CMV</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal"> را بررسی کرد و از وخامت بیماری آن‌ها جلوگیری کرد</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="font-weight:normal">. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">در این مطالعه، 200 نمونه پلاسمای (شامل 147 مرد و 53 زن) افراد </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">HIV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> مثبت جمع‌آوری و فراوانی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CMV-DNA</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در این افراد به روش </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">Real-time PCR</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> بررسی شد. سپس، داده‌ها از طریق نرم‌افزار</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">SPSS </span></span></span></i><i><span style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&nbsp;تحلیل آماری شد و ارتباط بین سن و جنس با فراوانی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CMV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> تعیین شد.</span></span></span></span></i><i>&nbsp; </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">فراوانی کلی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CMV-DNA</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در حدود 59% به دست آمد که با توجه به گستردگی دامنه سنی افراد مورد بررسی، شیوع نسبتاً بالایی است. این فراوانی در گروه‌های سنی 30-16 و 45-31 سال و در مردان نسبت به زنان بیشتر مشاهده شد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt">نتیجه</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt">گیری</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">در این تحقیق نشان داده شد که با توجه به بالا بودن شیوع </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">CMV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal"> در افراد </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">HIV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal"> مثبت باید توجه بیشتری به بررسی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">CMV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal"> با استفاده از تست‌های مولکولی در این افراد انجام شود و می‌توان از کاهش طول عمر و وارد آمدن آسیب‌های جدی ناشی از </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.1pt"><span style="font-weight:normal">CMV</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:&quot;B Nazanin&quot;"><span style="letter-spacing:-.1pt"><span style="font-weight:normal"> جلوگیری کرد.</span></span></span></span></i></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: HIV virus is known as one of the most important pathogenic viruses in human societies, which causes several kinds of opportunistic infections in patients due to immunodeficiency. One of the most important opportunistic viruses in these people is the CMV, which causes serious damage and shortens their lifespan. For this reason, rapid diagnosis of CMV in HIV positive people is important. In this research, it was shown that with a simple test in HIV positive people, it is possible to check the presence of CMV and prevent the deterioration of their disease. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: In this study, 200 plasma samples (147 males and 53 females) of HIV-positive people were collected and the frequency of CMV-DNA was checked by real-time PCR method. Then, the data were studied and analyzed through SPSS software, and the association between age and sex with the frequency of CMV was determined. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: The overall frequency of CMV-DNA was found to be around 59%, which is a relatively high prevalence considering the wide age range of the investigated subjects. This frequency was observed in the age groups of 16-30 and 31-45 years and in men more than women.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: It was shown that due to the high prevalence of CMV in HIV-positive people, more attention should be paid to the investigation of CMV using molecular tests in these people, and it is possible to reduce life shortening and serious damage caused by CMV.</span></span></span></span></span></span><br> &nbsp; CMV, HIV, AIDS, Real-time PCR CMV, HIV, AIDS, Real-time PCR. 234 240 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-1844-1&slc_lang=fa&sid=1 Reihaneh Kazemi ریحانه کاظمی re.kazemi71@gmail.com 100319475328460013455 100319475328460013455 No Department of Genetics, NT.C., Islamic Azad University, Tehran, Iran گروه ژنتیک، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران Kazem Baesi کاظم باعثی kbaesi@gmail.com 100319475328460013456 100319475328460013456 No Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران Mohammadreza Aghasadeghi محمدرضا آقاصادقی mr_sadeqi@yahoo.com 100319475328460013457 100319475328460013457 No Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران Seyed Amir Sadeghi سید امیر صادقی asadeghi765@gmail.com 100319475328460013458 100319475328460013458 No Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران Mojtaba Hamidi-Fard مجتبی حمیدی فرد mojtaba_hamidifard@yahoo.com 100319475328460013459 100319475328460013459 Yes Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران