fa بررسی همبستگی پلی‌مورفیسم جایگاه‌های BsmIو FokIژن گیرنده ویتامین D با خطر سرطان روده بزرگ در بیماران ایرانی مراجعه کننده به بیمارستان طالقانی تهران زيست شناسي مولكولي Molecular Biology موردي- شاهدي case-control <p style="LINE-HEIGHT: normal MARGIN: 0in 0in 0pt" dir="rtl" class="a" align="justify"><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><font face="Tahoma">z</font><strong>سابقه و هدف</strong></span><i><span style="FONT-FAMILY: Nazanin LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes" lang="FA">:</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="FA"> </span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA">ارتباط پلی­مورفیسم­های ژن گیرنده ویتامین </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">D</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA"><span dir="rtl"></span> و سرطان روده بزرگ، سینه و پروستات مشخص شده است. در این مطالعه همبستگی دو پلی­مورفیسم ژن </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">VDR</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA"><span dir="rtl"></span> در جایگاه</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA">های برش آنزیمی </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">BsmI</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA"><span dir="rtl"></span> و </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">FokI</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold"><span dir="rtl"></span> </span></i><span dir="ltr"></span><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><span dir="ltr"></span><span style="mso-spacerun: yes"><strong><font size="2" face="Times New Roman"> </font></strong></span></span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="AR-SA">با خطر ابتلا به سرطان روده بزرگ بیماران ایرانی بررسی شد</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">.</span></i><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-family: 'B Nazanin'" dir="ltr"><p></p></span></i></p><p style="LINE-HEIGHT: normal MARGIN: 0in 0in 0pt" dir="rtl" class="a"><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>روش بررسی</strong></span><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes" lang="FA">: </span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">در این مطالعه مورد- شاهدی، 110 نمونه </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">DNA</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span> افراد مبتلا به سرطان روده بزرگ و 110 نمونه افراد شاهد سالم جمع</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">آوری شد.</span></i><span dir="ltr"></span><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman"> </font></span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">تعیین ژنوتیپ به روش </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">PCR-RFLP</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span> انجام شد و برای تأئید نتایج </span></i><font face="Times New Roman"><i><span style="LETTER-SPACING: -0.3pt FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">RFLP</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span>، ژنوتایپ پنج درصد آنها با روش تعیین توالی مستقیم تعیین شد.</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes" lang="AR-SA"> </span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.3pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes" lang="FA"><p></p></span></i></p><p style="LINE-HEIGHT: normal MARGIN: 0in 0in 0pt" dir="rtl" class="a"><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.2pt FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>یافته</strong></span><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.2pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-weight: bold" lang="FA">­</span><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.2pt FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>ها:</strong></span><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal" lang="FA"> </span><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">فراوانی در پلی</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">مورفیسم</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">های جایگاه</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">های </span></i><font face="Times New Roman"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">BsmI</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span> و </span></i><font face="Times New Roman"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">FokI</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span> ژن گیرنده ویتامین </span></i><font face="Times New Roman"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr">D</span></i><span dir="rtl"></span></font><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span> میان بیماران و افراد شاهد سالم تا حد زیادی مشابه بود.</span></i><span dir="ltr"></span><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr" lang="AR-SA"><span dir="ltr"></span><font face="Times New Roman"> </font></span></i><span dir="rtl"></span><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span dir="rtl"></span><font size="3"><font face="B Nazanin"><span style="mso-spacerun: yes"> </span>توزیع اللی نیز در هر دو گروه مشابه گزارش شد. از لحاظ آماری تفاوت معنی</font></font></span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-weight: bold" lang="AR-SA">­</span></i><i><span style="FONT-FAMILY: 'B Nazanin' FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">داری میان بیماران دو گروه یافت نشد.</span></i><i><span style="FONT-FAMILY: 'B Nazanin' LETTER-SPACING: -0.2pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes" lang="AR-SA"> </span></i><i><span style="LETTER-SPACING: -0.2pt FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-family: 'B Nazanin'" dir="ltr"><p></p></span></i></p><p style="DIRECTION: rtl" align="justify"><span><span style="FONT-FAMILY: 'Times New Roman','serif' FONT-SIZE: 10pt mso-bidi-language: AR-SA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin' mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US"><em><font size="3"> <strong>نتیجه گیری: </strong>میان پلی مورفیسم Bsml و FokI ژن گیرنده ویتامین D وخطر سرطان روده بزرگ در بیماران ایرانی رابطه معنی داری وجود ندارد . برای تائید این نتایج وتعمیم آن به همه جمعیت ایرانی به انجام مطالعات گسترده با حجم نمونه بالاتری نیاز است.</font></em></span></span></p> <p><strong>Background:</strong> There are several studies that have identified relationship between VDR gene polymorphisms and colorectal (CRC) or other kinds of cancers, such as breast and prostate cancers. The aim of our study was to evaluate the association of VDR gene polymorphisms, BsmI and FokI, with colorectal cancer risk among Iranian patients. </p><p><strong>Materials and methods</strong>: In this case-control study, 110 DNA samples from Iranian CRC patients and 110 samples from healthy Iranian people. Genotyping of BsmI and FokI polymorphisms were performed by PCR-RFLP method. To confirm the RFLP results, 5% of samples were sequenced with direct sequencing method. </p><p><strong>Results</strong>: The frequency of the VDR gene polymorphisms at BsmI and FokI restriction sites in CRC patients and healthy controls was almost similar. Allele distribution in patients and controls was same. There was no statistically significant difference in genotype or allele frequency between CRC patients and control group. </p><p><strong>Conclusion:</strong> VDR FokI and BsmI genotypes are not associated with increased risk of colorectal cancer in Iranian patients. However, these data remain to be confirmed by studies with larger sample size in Iran </p> BsmI، FokI، گیرنده ویتامین D، سرطان روده بزرگ BsmI, FokI, Vitamin D receptor, Colorectal cancer 55 60 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-155-4&slc_lang=fa&sid=1 Pedram Azimzadeh پدرام عظیم زاده Azimzadeh.pedram@yahoo.com 10031947532846002907 10031947532846002907 Yes Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Mahdi Montazerhaghighi مهدی منتظرحقیقی 10031947532846002908 10031947532846002908 No Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Seyed Reza Mohebbi سیدرضا محبی 10031947532846002909 10031947532846002909 No Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Sara Romani سارا رومانی 10031947532846002910 10031947532846002910 No , Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Seyed Reza Fatemi سیدرضا فاطمی 10031947532846002911 10031947532846002911 No Gastroenterologist, Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Mohammad Amin Pourhoseingholi محمدامین پورحسینقلی 10031947532846002912 10031947532846002912 No Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Fatemeh Nemati Malek فاطمه نعمتی ملک 10031947532846002913 10031947532846002913 No Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی Mohammad Reza Zali محمدرضا زالی 10031947532846002914 10031947532846002914 No Gastroenterologist, Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran مرکز تحقیقات بیماری‌های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی