fa جستجوی ژن‌های موتاسین II و III I/ در استرپتوکوکوس موتانس‌های ایزوله شده از بیماران ایرانی ژنتيك Genetic تجربي Experimental <p style="LINE-HEIGHT: normal MARGIN: 0cm 0cm 0pt" dir="rtl" class="a"><a name="OLE_LINK147"></a><a name="OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>سابقه و هدف</strong></span></span></a><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: Nazanin FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">:</span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="ltr"></span><span dir="ltr"></span><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: Nazanin" dir="ltr" lang="FA"><span dir="ltr"></span><span dir="ltr"></span><font face="Times New Roman"> </font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">استرپتوکوکوس موتانس</span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"> </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA">که </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">در فضای دهانی وجود دارد، قادر به تولید باکتریوسین با خاصیت آنتی­بیوتیکی است. این باکتریوسین­ها در استرپتوکوکوس موتانس، موتاسین نام دارند. هدف از این مطالعه، جستجوی خاصیت آنتاگونیستی استرپتوکوک­های موتانس ایزوله شده از دهان بیماران ایرانی بود.</span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 14.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><p></p></span></i></span></span></p><p style="LINE-HEIGHT: normal MARGIN: 0cm 0cm 0pt" dir="rtl" class="a"><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: normal"><span dir="rtl"></span><span dir="rtl"></span><span style="mso-spacerun: yes"><font size="3" face="B Nazanin"><strong> </strong></font></span></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>روش بررسی</strong></span></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">: فعالیت آنتی­میکروبیال موتاسین­ها در مقابل استرپتوکوکوس موتانس</span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"> با </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="AR-SA"><span style="mso-spacerun: yes"><font size="3" face="B Nazanin"> </font></span></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><font face="Times New Roman">ATCC35668</font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"><span dir="rtl"></span><span dir="rtl"></span> با روش چاهک­گذاری و دیسک گذاری ارزیابی گردید. همچنین در 2/67 درصد موارد ژن­های کد کننده موتاسین تیپ <span style="mso-spacerun: yes"><font size="3" face="B Nazanin"> </font></span></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><font face="Times New Roman">II</font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"><span dir="rtl"></span><span dir="rtl"></span> و </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><font face="Times New Roman">I/ III</font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"><span dir="rtl"></span><span dir="rtl"></span> با روش </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><font face="Times New Roman">PCR</font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"><span dir="rtl"></span><span dir="rtl"></span> تعیین شدند. <p></p></span></i></span></span></p><p style="LINE-HEIGHT: normal MARGIN: 0cm 0cm 0pt" dir="rtl" class="a"><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>یافته</strong></span></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 14pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-ansi-font-weight: bold" lang="FA">­</span></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-bidi-font-weight: bold mso-no-proof: yes" lang="FA"><strong>ها:</strong></span></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span style="FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-bidi-font-family: 'Times New Roman' mso-ascii-font-family: Calibri mso-hansi-font-family: Calibri" lang="FA"><font face="Times New Roman"> </font></span></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA">در کل، <font size="3"><font face="B Nazanin"><span style="mso-spacerun: yes"> </span>از 32 نمونه استرپتوکوکوس موتانس ایزوله شده از دهان بیماران ایرانی، 25 سویه خاصیت آنتی­میکروبیال <span style="mso-spacerun: yes">  </span>موتاسین­های خود را نشان دادند و ژنوتایپ مثبت موتاسین­های تیپ 1، 2 و 3 این سویه­ها با روش </font></font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><font face="Times New Roman">PCR</font></span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><span dir="rtl"></span><span dir="rtl"></span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt" lang="FA"><span dir="rtl"></span><span dir="rtl"></span> تعیین شد. </span></i></span></span><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><i><span style="FONT-SIZE: 10pt FONT-WEIGHT: normal mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 12.0pt mso-bidi-font-family: 'B Nazanin'" dir="ltr"><p></p></span></i></span></span></p><p align="right"><span style="mso-bookmark: OLE_LINK146"><span style="mso-bookmark: OLE_LINK147"><b style="mso-ansi-font-weight: normal"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-fareast-font-family: 'Times New Roman' mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">نتیجه</span></b><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">­</span><b style="mso-ansi-font-weight: normal"><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-fareast-font-family: 'Times New Roman' mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">گیری</span></b><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ansi-font-weight: bold mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">:</span></i><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-bidi-language: FA mso-fareast-font-family: 'Times New Roman' mso-ascii-font-family: Calibri mso-hansi-font-family: Calibri mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA"> </span><i><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ansi-font-weight: bold mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">این مطالعه نشان داد موتاسین­های استرپتوکوکوس­های موتانس</span><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: AR-SA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ansi-font-weight: bold mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA"> </span><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: AR-SA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ansi-font-weight: bold mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="AR-SA">ا</span><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ansi-font-weight: bold mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">حتمالا دارای خاصیت آنتاگونیستی در مقابل رشد دیگر باکتری­ها می باشند. استخراج این ترکیبات (موتاسین­ها) می­تواند به عنوان یک داروی آنتی­باکتریال مورد استفاده قرار گیرد</span><span style="FONT-FAMILY: "B Nazanin" FONT-SIZE: 12pt mso-bidi-language: FA mso-no-proof: yes mso-ansi-font-size: 10.0pt mso-fareast-font-family: 'Times New Roman' mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US" dir="rtl" lang="FA">.</span></i></span></span></p> <p><strong>Background:</strong> Streptococcus mutans, resident in the oral cavity, is able to produce bacteriocins with antimicrobial properties, called mutacins. Aim of this study was to investigate the antagonistic properties of these bacteria isolated from Iranian patients.</p><p> <strong>Materials and methods:</strong> Antibacterial activities of mutacins were evaluated against S. mutans ATCC 35668 with disc diffusion and genes encoding mutacin types I, II and III was determined by PCR amplification method.</p><p> <strong>Results:</strong> Of 32 S. mutans isolated from caries-active and caries-free individuals, 25 strains demonstrated antimicrobial repertoire and 67.2 % showed positive genotypes by PCR.</p><p> <strong>Conclusion</strong>: This study showed that mutacins of Streptococcus mutans have antagonistic effects on the growth of other bacteria. Extract of this component could be used as antibacterial drug. </p> آنتی‌میکروبیال، موتاسین، استرپتوکوکوس موتانس. Antibacterial activities, Streptococcus mutans, Mutacin 89 96 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-1-339&slc_lang=fa&sid=1 Mitra Salehi میترا صالحی 10031947532846002998 10031947532846002998 No North Tehran Branch, Islamic Azad University, Tehran, Iran دانشگاه آزاد اسلامی، واحد تهران شمال Soheila Moradi سهیلا مرادی 10031947532846002999 10031947532846002999 No Razi Vaccine and Serum Research Institute, Karaj, Iran بخش میکروبیولوژی انسیتو رازی، کرج Tahereh Aghili طاهره عقیلی Shirin.Aghili.mi@gmail.com 10031947532846003000 10031947532846003000 Yes North Tehran Branch, Islamic Azad University, Tehran, Iran دانشگاه آزاد اسلامی، واحد تهران شمال Roya Razavipor رویا رضوی پور 10031947532846003001 10031947532846003001 No North Tehran Branch, Islamic Azad University, Tehran, Iran دانشگاه آزاد اسلامی، واحد تهران شمال