Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
The frequency of three common mutations of CARD15/NOD2 gene in Iranian IBD patients
107
112
FA
Farnood A
Y
Naderi N
N
Firouzi F
N
Rezvani MR
N
Javeri A
N
Bahari A
N
Aghazadeh R
N
Habibi M
N
Zali MR
N
Background: CARD15/NOD2 gene, located on the pericentromeric region of chromosome 16 (IBD 1) has been reported to have an association with IBD, especially Crohn’s disease (CD). Many independent studies have shown a variable association between three common mutations of CARD15, with Crohn’s disease in different ethnic groups. Thus, raising the hypothesis that genetic andor allelic heterogeneity may influence the relationship between CARD15 and Crohn’s disease. In the present study, we have investigated the frequency of three main mutations of CARD15 gene (Arg 702 Trp, Gly 908 Arg and Leu 1007 fsinsC) in Iranian IBD patients and compared it with healthy control population. Materials and methods: For this case-control study, 100 ulcerative colitis (UC), 40 Crohn’s disease patients and 100 sex-, age- and ethnicity-matched controls were enrolled from a teaching hospital during a one year period (2003-2004). All three mutations were assessed on DNA of leukocyte cells, by PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) methods.
Results: The mean age of UC, CD and healthy controls were 38.6±14.3, 36.6±14.1, and 38.6±14.2 years. Among the three evaluated CARD15 gene mutations, the frequency of Arg702Trp mutation was significantly higher in Iranian patients with Crohn’s disease (OR19.2 95%CI:4.2–87.3, p<0.001). None of these mutations were associated with ulcerative colitis.
Conclusion: This study showed that Arg702Trp mutation of CARD15 gene is probably associated with Crohn’s disease in Iranian population indicating that genetic polymorphisms may differ between populations.
http://tmuj.iautmu.ac.ir/article-1-3-en.html
http://tmuj.iautmu.ac.ir/article-1-3-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Evaluation of bi/oligoclonal rearrangement of immunoglobulin and T-cell receptor genes in Iranian children suffering B-precursor acute lymphoblastic leukemia
113
120
FA
Poopak B
Y
Poorfathoolah A
N
Najmabad H
N
Yahyavi SH
N
Mortazavi Y
N
Vosough P
N
Ansari Damavandi SH
N
Arjomandy Rafsanjani K
N
Arzanian MT
N
Izadyar M
N
Bahoosh GR
N
a shahgholi
N
Hamidieh AA
N
Faranoosh M
N
Khosravipoor G
N
Haghnejad F
N
Background: Clonal gene rearrangement of immunoglobulin and T cell receptor may have mono, bi or oligoclonal pattern. Significance of these patterns were studied at diagnosis and follow up of MRD in many countries, however, similar studies have not been conducted among Iranian patients. We investigated the bi/oligoclonal pattern and their association with quantitative and qualitative parameters especially MRD in Iranian children suffering from B-precursor acute lymphoblastic leukemia.
Materials and methods: In our prospective study, bone marrow aspirates of 140 patients with B-precursor ALLs were selected. Mononuclear cells including leukemic blasts isolated by density gradient. Having DNA extracted, hypervariable regions of IgH, IgK, TCR-δ (Dδ2-Dδ3,Vδ2-Dδ3) and TCR-γ (Vγ, VγI, VγII) were amplified by consensus primers using PCR. PCR products were analyzed after heteroduplex analysis and polyacrylamide gel electrophoresis (silver stain). The DNA sequences were compared and aligned to the sequences homologous for IgH and IgK published by Gene Bank. Bone marrow aspirates of days 14, 28 and 45, as well as months 3, and 6 were treated similarly.
Results: IgH gene rearrangements were reported in 114 (90.5%) patients using consensus primers for CDR-III and CDR-I regions (monoclonal:57.8%, biclonal:34.9% and oligoclonal:5.5%). Clonal pattern of Igκ-Kde were present in 59 cases (67%) (biclonal:10%). Clonal rearrangement of TCR-γ (Vγ) and VγI/II were present in 79.3% and 64.9% of patients, respectively, however, only 5% of cases showed biclonal pattern. The VγII rearrangement was the most common (46.8%) type in TCR-γ. 47 (45.2%) and 11 (16.6%) patients had Vδ2-Dδ3 and Dδ2–Dδ3 partial gene rearrangements, respectively. Biclonal/oligoclonal pattern were present in 13 (27.7%) and 2 (4.3%) cases with Vδ2-Dδ3 rearrangement. Only one patient had biclonal Dδ2-Dδ3 rearrangement. No significant difference regarding the quantitative and qualitative parameters and MRD was observed between the two groups.
Conclusion: Bi/oligoclonal rearrangement of IgH, IgK, TCR-δ (Dδ2-Dδ3,Vδ2-Dδ3) and TCR-γ (Vγ, VγI, VγII) genes had comparable pattern to other populations. Results of MRD study showed no significant differences between the two groups.
Gene rearrangement, Bi/oligoclonal, Acute lymphoblastic leukemia.
http://tmuj.iautmu.ac.ir/article-1-4-en.html
http://tmuj.iautmu.ac.ir/article-1-4-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Comparison of clinical manifestations and age and sex distribution in childhood acute lymphoblastic leukemia and acute myelocytic leukemia
121
124
FA
Hemmatyar M
Y
Haji-Naghdi M
N
Background: Acute leukemia is the most common childhood malignancy and acute lymphoblastic leukemia (ALL) has been reported five times more common than acute myelocytic leukemia (AML) among children. The present study addresses the clinical manifestations and age and sex distribution in childhood ALL and AML.
Materials and methods: This is a descriptive-analytic study performed on 70 ALL and 70 AML patients who have been hospitalized at Children Medical Center. Initial data were obtained through patients' files and analyzed by SPSS software and chi-square test.
Results: The mean age of patients with ALL was 5.1 years compared to 8 years in AML patients. Most of ALL patients aged under 5 years whereas AML patients aged ≥10 years (p<0.0001). In both groups, disease was more common in boys than girls. Paleness was the most frequent signs in both groups. Lymphadenopathy, hepatosplenomegaly and bone pain in ALL, and fever and bleeding in AML patients were more prevalent than other manifestations.
Conclusion: Leukemia must be considered in children with paleness and nonspecific signs. Although age distribution and some other clinical manifestations are differed between ALL and AML patients, these two entities could not easily differentiated according to the age, sex and clinical manifestations. In deed, definite diagnosis must be achieved with bone marrow aspiration or biopsy, fluocytometery and specific staining.
Acute lymphoblastic leukemia, Acute myelocytic leukemia, Clinical manifestations
http://tmuj.iautmu.ac.ir/article-1-5-en.html
http://tmuj.iautmu.ac.ir/article-1-5-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Nausea and vomiting after general anesthesia with halothane or isoflurane A double blind randomized trial
125
129
FA
Madine H
N
Moghani Lankarani M
Y
Assari Sh
N
Davoudi AM
N
Arti HR
N
Background: Postoperative nausea and vomiting (PONV) is potentially dangerous and causes morbidity and excessive costs to the patients. Prior investigators have reported controversies about halothane- and isoflurane-induced PONV, thus, the present study was conducted to compare these two drugs.
Materials and methods: 80 candidates for elective minor orthopedic surgery were randomly allocated into two groups, halothane (n= 40) and isoflurane (n=40). Confounding factors including sex, age, duration of surgery, NPO, history of general anesthesia, history of PONV, drugs, and history of disease were recorded in a questionnaire. PONV was assessed at three occasions (recovery, 3- and 6-hour post operative).
Results: PONV was reported in 4 (10%) and 3 (7.5%) patients in halothane and isoflurane groups, respectively (NS). None of the patients suffered PONV 3- or 6-hour post operative.
Conclusions: Results revealed that the incidence of PONV can be hold low, with an aggressive control of risk factors, in addition, halothane or isoflurane seems to have no preference from this aspect to each other.
General anaesthesia, Halothane, Isoflurane, Postoperative nausea and vomiting
http://tmuj.iautmu.ac.ir/article-1-6-en.html
http://tmuj.iautmu.ac.ir/article-1-6-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
1470 cases of sarcoma referring to Imam Khomeini hospital during an 11-year period
131
136
FA
Seddighi S
Y
Rafat J
N
Background: Sarcoma is a malignant neoplasm arising in mesenchymal tissue. It comprises 1% of adult malignancies and 15% of childhood neoplasms. The present study was designed to review the clinical characteristics of 1470 sarcoma cases and related predictors of out come, relapse and survival.
Materials and methods: For this retrospective study, 1470 medical files of patients with sarcoma who had referred during 1991–2002 to Imam Khomeini Hospital were studied.
Results: Sarcoma comprised 11% of all cases referring to our hospital. The mean age of patients with sarcoma was 30 years and male to female ratio was 3/2. Bone to soft tissue sarcoma ratio was 3.1 in children and 1.3 in adults. Osteosarcoma, Ewing and rhabdomyosarcoma presented 83% of children tumors, however, in adults osteosarcoma, synovial sarcoma and malignant fibrous histocytoma (MFH) were the most common subtypes. The main prognostic factors for survival were tumor size, margin of surgery, neurovascular involvement in pathological report, initial metastasis and complete response to initial therapy. Adjuvant radiotherapy, small tumor size, curative surgery with chemotherapy and free surgical margins were significantly associated with reduced recurrence.
Conclusion: Complete response to primary therapy is the main independent variable of overall survival of patients. Earlier diagnosis and experienced team including surgical, medical and radiotherapy oncologist is needed for better response and longer survival rate.
Sarcoma, Prognosis, Treatment.
http://tmuj.iautmu.ac.ir/article-1-7-en.html
http://tmuj.iautmu.ac.ir/article-1-7-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Association between renal graft survival and age, sex and anatomical position
137
140
FA
Iranpour A
Y
Dadashi EinSheikh Z
N
Background: Renal transplantation is the treatment of choice for patients with end stage renal disease (ESRD). The present study was designed to evaluate the association between renal graft survival and age, sex and anatomical position.
Materials and methods: For this historical cohort study, 129 patients who have received renal transplantation during a 5-year period (1998-2003) in Modarres Hospital in Tehran were included through a non probability sampling. The creatinine level one year following the transplantation was considered for graft outcome. Meanwhile, graft rejection, death, transfusion and hypertension before and after transplantation have been evaluated.
Results: Renal transplantation was successfully achieved in 79.8% of cases. Reduction in blood pressure following the transplantation and transfusion was not significantly differed between the female and male, left- and right-kidney, and older and younger than 28 years old recipients. The minimum rejection rate was observed in male-to-female group however, the minimum mortality rate was reported in female-to-female group. Decreased serum creatinine level was appeared earlier in the transplant’s recipients of left-kidney and female subjects.
Conclusion: We suggest longer follow up of blood pressure and kidney transplantation from donor with brain death to improve renal graft survival.
Kidney anatomic position, Kidney transplantation, Graft rejection
http://tmuj.iautmu.ac.ir/article-1-8-en.html
http://tmuj.iautmu.ac.ir/article-1-8-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Surveying the association between computer–video games and aggression in male students of guidance schools in Tehran, 2003
141
145
FA
Abdolkhaleghi M
Y
Davachi A
N
Sahbaie F
N
Mahmoudi M
N
Background: One of the most exciting fields of mankind life in the recent century is computer–video games that has an increasing popularity among children and adolescents. However, it has incited the general anxiety from the probable destructive effects on these age groups. One of the major concerns is the obvious feature of aggressiveness. The present study was designed to survey the association between computer–video games and aggression in male students of guidance schools in Tehran.
Materials and methods: The study population included 333 male students of guidance school who have been randomly selected. They were requested to fill both the research and aggression questionnaires.
Results: Results have revealed a non-significant direct association between aggression and computer-video games. However, there was a significant relationship between the time of proceeding to these games and type of game with the types of aggression in a way that increasing the rate of proceeding to these games and harsh games, the rate of student’s aggression was increased.
Conclusion: Our results showed that encountering to the computer games continually may cause the manners of aggression and decreasing of educational function.
Computer–video games, Students, Aggression.
http://tmuj.iautmu.ac.ir/article-1-9-en.html
http://tmuj.iautmu.ac.ir/article-1-9-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Surveying the frequency of chest trauma (blunt and penetrating in Air Force Hospital, 2002–2004
147
150
FA
Y
N
N
Background: Trauma is the most common cause of death, admission and disability in all age groups ranging between 1-40 years. The present study was conducted to survey the frequency of chest trauma (blunt and penetrating) in Air Force Hospital in a two-year period, 2002–2004.
Materials and methods: A retrospective study was conducted at Surgery and Emergency departments of Air Force hospital on all consecutive (blunt and penetrating) chest trauma patients treated between March 2002 and March 2004.
Results: Totally, 814 cases referred with chief complain of chest trauma. Blunt chest trauma was reported in 78.1% of cases (636 patients) and penetrating chest trauma in the remaining 21.9% (178 patients). Totally, 84.7% were males and 76.4% aged 14-40 years. The most common site of injury was chest wall (90.3%) and soft tissue injury was the most prevalent form of trauma (31.6%). Extremities were the most common site of co-injury (13.3%). Car accident was by far the most frequent cause of trauma (57.9%). The mortality rate was 3.8% (31 patients).
Conclusion: Car accident was the most common cause of chest trauma. Meanwhile, soft tissue injury was the most frequent type of injury.
Trauma, Chest, Blunt trauma, Penetrating trauma
http://tmuj.iautmu.ac.ir/article-1-10-en.html
http://tmuj.iautmu.ac.ir/article-1-10-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Prevalence of intestinal parasites in Ghaemshahr in 2004
151
155
FA
Ranjbar-Bahadori Sh
Y
Dastorian AR
N
Heidari B
N
Background: Parasitic infections, especially in under developing countries, are major health concerns. They are of utmost importance in our country due to geographical location, suitable weather and cultural background. This study intends to investigate the prevalence of intestinal parasites in urban and rural population of a northern city, Ghaemshahr. Materials and methods: The study population included 4898 urban and 1697 rural residents. Stool samples were randomly obtained and examined by direct and flotation methods. The data were analyzed by chi-square.
Results: A total of 557 subjects (8.4%) were infected with intestinal parasites among whom 2.8% had protozoa and 5.7% had intestinal helminthes infections. Giardia lamblia was the most frequent parasite (32.8%). Totally, 7.9% of males and 9% of females were infected (NS). Meanwhile, 10.6% of rural subjects and 7.7% of urban residents were infected (p<0.001).
Conclusion: Regarding to the high rate of infection with Giardia lamblia, filtration of drinking water is strictly recommended. Meanwhile, our study showed a higher prevalence of parasitic infections in the rural area, therefore, general education and promoting hygienic situation may be partly helpful to control the situation.
Intestinal parasites, Prevalence, Age, Sex, Ghaemshahr, Iran
http://tmuj.iautmu.ac.ir/article-1-11-en.html
http://tmuj.iautmu.ac.ir/article-1-11-en.pdf
Islamic Azxad University, Tehran Medical Branch
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
1023-5922
2008-3386
15
3
2005
9
1
Report of a patient with concomitant abdominal aortic aneurysm and colorectal carcinoma
157
160
FA
Mansoori S
Y
Sami H
N
Moeini A
N
Moein Jahromi E
N
We present a 73 year-old severely ill Iranian patient who had a cardiac arrest and a successful cardiopulmonary resuscitation(CPR) one hour after admission in N.I.O.C Hospital. He was a known case of abdominal aortic aneurysm which was accidentally diagnosed by ultrasonography two years ago, so he was emergently operated with probable diagnosis of rupture of aneurysm. Meanwhile, he had a history of painless rectorrhagia unrelated to defecation during the past six months. Tissue diagnosis of rectal adenocarcinoma was defined following a total colonoscopy. Colorectal carcinoma and abdominal aortic aneurysm are common diseases in similar age group. Co-existence of these diseases is rare. Treatment strategy is a big challenge for these patients.
In this patient, aneurysmectomy was performed first, because of emergent condition of the patient, and treatment of the colorectal carcinoma was postponed.
Abdominal aortic aneurysm, Colorectal carcinoma, Treatment.
http://tmuj.iautmu.ac.ir/article-1-12-en.html
http://tmuj.iautmu.ac.ir/article-1-12-en.pdf