1 1023-5922 Islamic Azxad University, Tehran Medical Branch 1292 Genetic Glioblastoma gene therapy and related delivery systems Noori-Daloii Muhammad-Reza b Ahmadi-beni Reza c b Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran c Department of Medical Genetics, School of Medicine 1 9 2017 27 3 149 163 22 05 2017 25 07 2017 Gene therapy of glioblastoma multiforme is based on direct introduction of gene(s) to achieve anti-tumor effects and four types of it are examined: (A) suicide gene therapy; (B) immunomodulatory gene therapy; (C) tumor-suppressor gene therapy and (D) oncolytic gene therapy. Now, viral and nonviral vectors, tumor-tropic cell carriers with therapeutic gene(s) expression ability, and “intelligent” carriers, increase delivery, specificity, and toxicity against GBM. In this review, by using dozens of valid and updated academic papers and personal experiences of gene therapy methods and delivery systems relevant in glioblastoma, and its prospects are discussed. Also interesting prospective clinical trials of gene therapy for glioblastoma are mentioned.
1294 Physiology Protective effect of L-carnitine and gallic acid in rat model of renal ischemia/reperfusion injury Sadri Sara d Edalatmanesh Mohammad Amin e d Department of Biology e Department of Biology 1 9 2017 27 3 164 170 28 02 2017 30 04 2017 Background: Acute kidney injury (AKI) often occurs as a result of ischemia-reperfusion. AKI is a major renal disease associated with high mortality rate. We investigated the renal protective effects of gallic acid and L-carnitine on the blood factors in rat model of renal ischemia-reperfusion. Materials and methods: In this experimental study, forty male Wistar rats were randomly divided into 4 groups: controls, ischemia/reperfusion group (I/R), ischemia-reperfusion+L-carnitine (I/R+Lc, 500mg/kg), and ischemia/reperfusion + gallic acid (I/R+GA, 100mg/kg). Animals of the experimental groups were subjected to unilateral nephrectomy and received Gallic acid and L-carnitine for 14 days. Then, ischemia was induced by clamping the renal pedicle for 45 minutes. 72 h later, they were sacrificed, and the blood level of BUN, uric acid and creatinine were analyzed. Results: The results showed a significant increase in BUN and creatinine of I/R group compared to the controls. Also, BUN and creatinine were significantly decreased in I/R+Lc and I/R+GA groups in comparison with I/R group. On other hand, blood level of uric acid was decreased in I/R+GA group compared to I/R group. Conclusion: The results indicated that ischemia/reperfusion induced acute renal injury in rat model, and pretreatment with L-carnitine and gallic acid can prevent the kidney tissue damage from renal ischemia. Keywords: Nephrectomy, Ischemia/Reperfusion, L-carnitine, Gallic acid, Rat. 1295 Physiology Protective effects of fish oil omega3 supplements and aqueous extract of Glycyrrhiza glabra root against changes of liver enzymes levels induced by thioacetamide in male rats Moghadamnia Davood f moghadamnia davood g Mokhtari Mokhtar h Khatamsaz Saeed i f Ph.D in Animal Physiology g Ph.D in Animal Physiology h Department of Biology, Kazerun Branch i Department of Biology ,Kazerun Branch 1 9 2017 27 3 171 177 05 10 2016 26 12 2016 Background: Thioacetamide is a liver toxin that causes centrilobular necrosis. In this study, the protective effect of fish oil omega3 supplements and aqueous extract of Glycyrrhiza glabra root against disorder in liver enzymes level induced by thioacetamide in male rats was investigated. Materials and methods: In this experimental study, 63 male rats were divided into 9 groups: The control group, the sham group, the thioacetamide group, the experimental groups 1-6. Experimental groups received a daily dose of 100, 200 and 300 mg/kg fish oil omega-3 supplements and the aqueous extract of Glycyrrhiza glabra root orally respectively for 3 month followed by an inter peritoneal dose of 150 mg/kg thioacetamide at the end of the experiment. The serum levels of SGOT, SGPT, ALP, GGT and LDH were measured. Results: The serum level of SGPT showed a significant decrease in the experimental groups 1, 5 and 6 compared to the group received thioacetamide. The serum level of GGT showed a significant decrease in the experimental groups 1, 2, 5 and 6 compared to the group received thioacetamide. The serum levels of SGOT, ALP and LDH in all experimental groups did not show significant changes compared to the group received thioacetamide (P<0.05). Conclusion: Fish oil omega3 supplements and aqueous extract of Glycyrrhiza glabra root had protective effects against disorder in liver enzymes level induced by thioacetamide in male rats. Keywords: Glycyrrhiza glabra root, Omega3, Thioacetamide , Liver enzymes, Male rats 1296 Physiology Investigating cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line Behdarvand Shoushtar Anahita j Sazgar Hossein k Ghasemi Pirbaloti Abdollah l j M.Sc of Animal Physiology, Department of Biology, Faculty of basic Science k Ph.D of Animal Physiology, Department of Biology, Faculty of basic Science l Ph.D of Medicinal and Aromatic Plants, Department of Medicinal Plants 1 9 2017 27 3 178 186 26 12 2016 20 09 2017 Background: Cervical cancer is the most common gynecologic cancer and the second most common cancer in women worldwide. Herbal preparations are used to treat cancer for centuries. The aim of this study was to investigate the cytotoxic effect of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge on Hela cancer cells line. Materials and methods: Hela cancer cells line and fibroblast normal cells line were cultured respectively in RPMI 1640 medium and DMEM medium with fetal bovine serum (FBS) and antibiotics. The cells exposed to different doses of hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25 and 2.5 mg/ml) and were incubated for 24, 48 and 72 hours. After the incubation period, modified colorimetric MTT method was used to determine the cellular toxicity of the extract. Results: The results of the MTT test showed that hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge had dose-dependent and time-dependent anti-cancer effect on Hela cells line. The highest percentage of cell death was observed after 72 hours incubation and increase in extract concentration (p<0.05). Extract did not show significant cytotoxicity on fibroblast normal cells line. Conclusion: Hydroalcholich extract from combination of Kelussia odoratissma Mozaff and Satureja bachtiarica Bunge has cytotixic effect on Hela cells line. However this extract does not have any cytotoxic effect on fibroblast normal cells line. It seems that further researches can lead to detect effective compounds in the cancer treatment. Keywords: Kelussia odoratissma Mozaff, Satureja bachtiarica Bunge, MTT assay, Hela 1297 Anatomy Effects of losartan on spatial memory deficits induced by global ischemia / reperfusion in male wistar rats Movassaghi Shima m Movassaghi Shabnam n Yousefi Oudarji Ali o Nadia Sharifi Zahra p m School of Medicine n Anatomical Sciences Department, School of Medicine o Anatomical Sciences Department, School of Medicine p Anatomical Sciences Department, School of Medicine 1 9 2017 27 3 187 193 04 11 2016 14 02 2017 Background: Stroke is the most important reason of cerebral ischemia and following reperfusion lead to programmed cell death. Harm to hippocampus causes major disruption in the performance of active memory tests. So in this study, we investigated the effect of losartan (angiotensin antagonists II) on spatial memory deficits and neuronal damage caused by ischemia /reperfusion in rats. Materials and methods: In this experimental study, animals were divided into 4 groups as follow: control, ischemia, experimental and vehicle. 20 minutes before ischemia, 5mg/kg losartan was injected to animals in experimental group. Ischemia was done by both common carotid occlusion for 20 minutes followed by reperfusion. After a week, rats were tested by Morris water maze and histological changes of CA1 pyramidal cells were studied by Nissl and Tunnel staining. Results: Results of Morris water maze showed significant difference between control and ischemia groups, while this difference was not significant between control and experimental groups. There was significant difference in the number of viable pyramidal neurons and apoptotic cells in CA1 region of hippocampus between control and experimental groups compared with vehicle and ischemia groups. Conclusion: 5mg/kg losartan improves pyramidal cells and histological changes of CA1 region caused by ischemia/ reperfusion and can prevent apoptosis, but this dose cannot reduce behavioral disorders caused by cerebral ischemia. Keywords: Cerebral ischemia, Losartan, Spatial memory, Neuroprotective. 1298 psychology Efficacy of music therapy on anxiety and diarrhea among patients with irritable bowel syndrome Arvand Jinous Sakhayi Ashraf Jadidi Hooshang PhD of Consultation, Department of Psychology MA of Consultation, Department of Psychology PhD of Consultation, Department of Psychology 1 9 2017 27 3 194 200 16 01 2017 22 02 2017 Background: Irritable bowel syndrome (IBS) is one of the most prevalent disease worldwide and it is considered as a stress- related disorder. Currently, music therapy is used for many somatic and psychological disorders. In this study, we evaluated efficacy of music therapy on anxiety and diarrhea among patients with IBS. Materials and methods: In this quasi experimental study, 80 female IBS patients with age of 30-45 years were randomly assigned into two groups of intervention (n=40) and control (n=40). Two groups continued drugs prescribed by physician. Intervention group received music therapy in groups of 8-10 people. Immediately before and at the end of study, all patients fulfilled Beck anxiety inventory and were asked for frequency of diarrhea. Data were analysed by ANCOVA using SPSS-ver20 software. P<0.05 was considered statistically significant. Results: Mean (± standard deviation) of anxiety score in intervention and control groups was 22.32±4.96 and 23.4±5.94 before study, which reached to 16.32±6.97 and 21.6±6.6 at the end of study respectively (p<0.0001). Mean (± standard deviation) of diarrhea frequency in intervention and control groups was 3.79±0.3 and 3.01±0.4 before study, which reached to 1.59±01 and 2.87±0.1 at the end of study respectively (p<0.0001). Conclusion: This study showed that music therapy is a useful technique on decreasing anxiety and diarrhea in IBS patients and improves quality of life of these patients. Keywords: Irritable bowel syndrome, Music therapy, Anxiety, Diarrhea. 1299 Health Information Management The study of traffic accidents in Zanjan Province between March 2009 and February 2016 and comparing the mathematical method to predict traffic injuries referred to the Forensic Medicine between 2017 and 2020 Omidi Nabi Asgari Heshmatolaah Omidi Mohammad Reza Jafari Eskandari Meysam Department of Management Faculty of Humanities Lecturer, Department of Industrial Engineering Department of Industrial Engineering 1 9 2017 27 3 201 208 08 10 2016 20 09 2017 Background: Accidents cause mortality and severe damage to life and property. Heavy consequences of social, economic, cultural and human societies is seriously threatened, which is important for future studies in the field of traffic accidents. Materials and methods: In this study, using gray system GM (1,1), gray circulation model RGM (1,1), gray model FGM (1,1), remained the same model, ARIMA time series model and the harmonic pattern, as well as the use of statistical data, by the number of victims of traffic accidents in the province between March 2009 and February 2016, the number of victims referred to the coroner province between 2017 and 2020 was predicted. Results: MAPE for models GM, RGM, FGM, changes survivor, ARIMA and harmonics to predict the number of patients referred to the 0.10, 0.07, 0.12, 0.14, 0.13 and 0.15, respectively. It indicates a more accurate method of RGM the anticipated number of patients referred to the coroner in the province of Zanjan. The number of victims in the year of 2009 reaches to 2894 people. Conclusion: The RGM is the best method to predict the number of disaster victims referred to legal medicine of Zanjan province, compared to other methods of case study. Reducing the number of disaster victims referred to legal medicine represents the improvement of driving tips and actions and nested in the province. Keywords: Future of research, Forecasting, Data mining, Safety, Accidents, Forensic medicine. 1300 Anesthesiology Evaluation the effect of exchange time anesthetic circuit anti- bacterial filters, on microbial culture results of anesthetic circuits in Bouali Hospital operating theaters Gholami Farshid Narimani Zaman Abadi Mahnaz Seyed Mehdi Seyed Ahmad Aryafar Mohammad Ansari Ladan Shakiba Majid Bu-Ali Hospital, Department of Anesthesiology Bu-Ali Hospital, Department of Anesthesiology Bu-Ali Hospital, Department of Anesthesiology Bu-Ali Hospital, Department of Anesthesiology Bu-Ali Hospital, Department of Anesthesiology Bu-Ali Hospital 1 9 2017 27 3 209 212 31 08 2016 30 04 2017 Background: To prevent the anesthesia circuit from bacterial colonization, during the time, we used to have an antibacterial filters with no predicted changing algorithm. In this study, we evaluated the effect of exchange time anesthetic circuit anti- bacterial filters, on microbial culture results of anesthetic circuits in Bouali Hospital operating theaters. Materials and methods: During a descriptive-analytic study, we changed the antibacterial filters in 4 different operating rooms; per patients at 1st room, daily at 2d room, 2times a week at 3rd room & weekly at the last one and kept them for the first week of study. At the rest of time until 10 months, we changed the filters in a manner to equal changing algorithm on all 4 rooms. we had microbial culture exam from the Y-piece & Mount-catheter every week ends. Results: Changing filter algorithm were 243 surgeries (25.5%) per patient (group1), 243 surgeries (25.5%) daily (group2), 237 surgeries (24.6%) twice a week (group3) and 241 surgeries (25%) weekly (group 4). The positive culture results were obtained on 21 operations (2.2%) in Y-piece and 27 operations (2.8%) in Mount-catheter. Conclusion: When we consider the whole number of surgeries in one day as a unit, positive culture results on Y-piece in each group would be 1(2.6%) for group1, 0 for group 2 , 2 (5%) for group 3, 1(2.5%) for group 4 (P=0.9). For Mount-catheter positive culture results were 2(5.1%) for group 1, 0 for group 2, 1(2.5%) for group 3 and 1(2.5%) for group 4 (P=0.6). No differences were found on concomitant positive culture tests from Y and Mount pieces. Keywords: Microbial culture, Anesthesia circuit, Anti-bacterial filter. 1302 Medical Physics Visual evoked potential in subjects under radial keratotomy and those without refractive errors surgery Kamalhosseini Arghavan Sadat Medical Student, Department of Medicine, Tehran Medical Scences Branch, Islamic Azad University, Tehran, Iran Department of Biophysics and Medical Physics, Tehran Medical Scences Branch, Islamic Azad University, Tehran, Iran 1 9 2017 27 3 213 216 05 12 2016 06 03 2017 Background: Radial keratotomy is a major type of refractive surgery. This study was performed to determine the association of visual evoked potential (VEP) and radial keratotomy. Materials and methods: In this cross-sectional descriptive-comparative survey, 25 patients under radial keratotomy attending to Basir Clinic in 2014 and 2015 were enrolled and the VEP results, including voltage and latency, were determined and compared with 25 control subjects. Results: The mean voltage was 5.6 ± 2.0 microvolt and 6.0 ± 2.2 microvolt in case and control groups, respectively (P>0.05). Mean latency duration was 95.7 ± 6.1 millisecond and 95.6 ± 5.7 millisecond in case and control groups, respectively (P>0.05). Conclusion: Totally, according to the obtained results, it may be concluded that there is no significant association between visual evoked potential and radial keratotomy. Keywords: VEP, Refractory surgery, Radial keratotomy 1303 Infectious Diseases Barriers and motivators factors effecting influenza vaccination uptake among healthcare workers Darvishi Mohammad Hajiparvaneh Robabeh Mohammadi Mohsen Akhlaghdoust Meisam Akhlaghdoust Meisam Infectious diseases & Tropical Medicine Research Centre (IDTMRC), AJAUMS, Tehran, Iran MS of ICU, Besaat Hospital, Tehran, Iran MD, Minimally Invasive Techniques Research Center in women, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran MD, Minimally Invasive Techniques Research Center in women, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran Pars Advanced and Minimally Invasive Medical Manners Research Center, Pars Hospital, Tehran, Iran 1 9 2017 27 3 217 221 21 11 2016 15 04 2017 Background: The use of influenza vaccine in healthcare staff was suggested, but the studies have shown that frequency of vaccination is low in healthcare staff. In this study, the application of the vaccination have been studied in healthcare staff of the “Besaat” Hospital. Materials and methods: In this descriptive study, 154 healthcare staff were studied at "Besaat" Hospital. The data were collected by 2 questionnaires and then the correlation coefficient of Pearson was evaluated. Results: Distrust available vaccines, fear of side effects, lack of enough information in vaccination-time and lack of enough time for receiving the vaccine were the most important preventive causes in application of the vaccine. Conclusion: This study showed that vaccine with valid brand, distribution of vaccine in proper time and season, presentation of free vaccine to personnel and their family, management of vaccine in all shifts, holding educational seminars and active information system are effective encouraging items in application of vaccine. Keywords: Influenza, Vaccination, Barriers, Motivators, Healthcare workers.