2024-03-29T05:46:50+03:30
http://tmuj.iautmu.ac.ir/browse.php?mag_id=54&slc_lang=fa&sid=1
54-1247
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Assessment of factors affecting kidney transplant survival based on literature review between 2000 and 2014
Reza
Rabiei
Hamid
Moghaddasi
Ali Akbar
Khadem Maboudi
Abbas
Basiri
Shahabedin
Rahmatizadeh
sh.rahmatizadeh@sbmu.ac.ir
Background: Improvements in immune-suppressing drugs have a short-term effect on kidney transplants but do not have much long-term effect. In order to increase the duration of kidney transplant survival, understanding the significant parameters is of great importance. This study aims to identify key parameters (before the transplant occurs) which affect kidney transplant survival.
Materials and methods: To conduct this review, the scientific databases was searched and more than 200 related titles were retrieved; and the abstracts were reviewed to determine which studies were relevant to the study. The literature analysis was based on the goals of the studies themselves, the number of citations, and the reliability of the sources cited. Ultimately, 60 studies included for further analysis.
Results: Based on the frequency of each parameter which influenced graft survival, the following parameters were determined to have higher frequencies compared to other parameters, with regards to survival time of the kidney transplant: the ages of the donor and recipient, socioeconomic status, immune-suppressing drugs, HLA matching, time on the waiting list, live or deceased donor, the decade in which the operation occurred, cold ischemia time, and reaction to antibiotics.
Conclusion: The influence of some of the kidney transplant survival parameters was acknowledged in the majority of studies; however, some parameters have been the subject of disagreement among studies. In order to resolve this issue, we suggest a search of international data and meta-analysis of current literature to better understand the parameters of kidney transplant survival.
Keywords: Kidney transplant, Kidney transplant survival, Factors affecting kidney transplant survival.
Kidney transplant
Kidney transplant survival
Factors affecting kidney transplant survival.
2017
6
01
77
87
http://tmuj.iautmu.ac.ir/article-1-1247-en.pdf
54-1248
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
The protective effect of ethanol extract of ginger (Zingiber officinale) on spermatogenesis toxicity induced by doxorubicin
Fatemeh
Juibar
Mokhtar
Mokhtari
M.Mokhtari246@yahoo.com
Mehdi
Dianatpur
Mehrdad
Shariatie
Background: DOX is one of the most common drugs used to treat different types of cancer, including testicular cancer. The aim of this study was to evaluate the effect of ethanol extract of ginger on spermatogenesis in rats.
Materials and methods: In this experimental study, 56 male rats Wistar were randomly divided into 6 groups of 8 animals control, the sham and experimental group 1 receiving doxorubicin (mg/kg.bw 3) experimental 2 and 3 receiving ginger extract (500 , 1000 mg/ kg. bw ) and experimental 4 and 5 received a ginger extract (500 , 1000 mg/ kg. bw ) + doxorubicin. The rats were fed extract daily, one hour after doxorubicin injection, for four weeks. DOX (3mg/kg) was administered once a week intraperitoneally. Some sperm parameters, such as the number, viability, morphology and mobility were examined using Eosin-Ngrozin staining.
Results: A significant decrease in body weight, sperm count, sperm motility, viability was observed in drug-DOX-treated rats compared with the control group. The reduction in the group treated with DOX with ginger 500 and 1000 mg/ kg significantly was offset compared with DOX.
Conclusion: Ginger is able to have protective effect on sperm parameters in adult male rat induced by Doxorobicin.
Keywords: Ginger, Doxorubicin, Body weight, Testes, Sperm parameters, Rat.
Ginger
Doxorubicin
Body weight
Testes
Sperm parameters
Rat.
2017
6
01
88
96
http://tmuj.iautmu.ac.ir/article-1-1248-en.pdf
54-1249
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
The evaluation of hippocampal level of brain derived neurotrophic factor and spatial memory in valproic acid animal model of autism
Zahra
Borzu
Mohammad Amin
Edalatmanesh
amin.edalatmanesh@gmail.com
Background: Autism, a behavior-developmental disorder, is characterized by impairments in social interaction, communications, as well as restricted, repetitive, and stereotyped patterns of behavior. The studies revealed changes in hippocampal level of brain-derived neurotrophic factor (BDNF) in autism etiology. However, their level and mechanism of action are needed to be elucidated. This study aimed to determine the hippocampal levels of BDNF and its relation with spatial memory in valproic acid animal model of autism.
Materials and methods: In this experimental study, 40 male and female Sprague Dawley rat pups were divided into two Phosphate-Buffered Saline receiver (PBS, n=20) and Valproic Acid receiver (VPA, n=20) groups. VPA model of autism was induced by intra-peritoneal administration of VPA (500 mg/kg) at 12.5 days after gestation. To measure changes in spatial memory Morris water maze test was used. Then, the hippocampal levels of BDNF were determined by ELISA method.
Results: Increased spatial memory was observed in Morris water maze test among VPA group compared to PBS group. In addition, the hippocampal levels of BDNF in VPA rats were significantly higher than PBS group (p<0.05).
Conclusion: Increasing BDNF showed enhancement of spatial memory in VPA rat model of autism.
Keywords: Autism, Valproic acid, BDNF, Spatial memory.
Autism
Valproic acid
BDNF
Spatial memory
2017
6
01
97
104
http://tmuj.iautmu.ac.ir/article-1-1249-en.pdf
54-1250
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Evaluation of serum angiotensin converting enzyme (ACE) activity in type 1 Gaucher’s patient and its relationship to ACE gene insertion/deletion in intron 16
Morteza
Ghazanfari Jajin
Shohreh
Khatami
Hadi
Mozafari
Abolfazl
Fateh
Maryam
Mobaraki
Mohammad
Taghikhani
Background: Gaucher's disease is an autosomal recessive disease which is the result of mutations in the β glucocerebrosidase gene. The aim of this study was to evaluate activity level of ACE enzyme Iranian patients with Gaucher’s disease type I, and also polymorphism I/D in intron 16 of ACE gene, as a marker in diagnosis and monitoring of disease.
Materials and methods: The experiments were performed on 29 patients (mean age of 10.04 years) and 60 healthy subjects (mean age of 7.31 years). Procedures included DNA extraction from blood, detection of polymorphism I/D by PCR and evaluation of activity level of ACE enzyme.
Results: The mean of ACE activity was 231.07 U/L which was increased 4 times than normal status (56.03 U/L). Evaluation of polymorphism I/D of the 29 patients showed t6 (20.7%) II, 9 (31%) DD and 14 (48.3%) ID (p<0.05).
Conclusion: According to the results, the measurement of the ACE activity levels can be used as cofactors in diagnosis and as well as an important factor in the monitoring of treatment. Polymorphism I/D with respect to the role of the ACE activity can be effective in increasing the specificity of the experiments.
Keywords: Gaucher's disease, Angiotensin converting enzyme (ACE), β- glucocerebrosidase, Polymorphism I/D.
Gaucher's disease
Angiotensin converting enzyme (ACE)
β- glucocerebrosidase
Polymorphism I/D.
2017
6
01
105
112
http://tmuj.iautmu.ac.ir/article-1-1250-en.pdf
54-1251
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Anticancer effects of some Girard-P reagent-based schiff bases and their Dimethyltin (IV) complexes on malignant glioblastoma cell line in in vitro culture
Elham
hoveizi
e.hoveizi@yahoo.com
Zynab
Ansariasl
Background: Glioblastoma (U-87) is the most common and most malignant of the glial tumors that appeared in the central nervous system. This is the first study that has evaluated the cytotoxic effects of various Girard-P reagent-based Schiff bases and their dimethyltin (IV) complexes on Glioblastoma cell line, U-87, viability.
Materials and methods: In this experimental study, U-87 cell line was grown in DMEM supplemented with 10% FBS, penicillin/streptomycin (100 U/ml, 100 µg/ml) at 37 ◦C in 5% CO2, then the effects of different concentrations of complexes on Glioblastoma cell were evaluated by MTT and DAPI staining.
Results: Herein, we demonstrated that these compounds had cytotoxicity effects of Glioblastoma cancer cells. In a dose dependent manner, a significant cytotoxicity was observed with increasing of P1M and P3.
Conclusion: The results showed that Girard-P reagent-based Schiff bases and their dimethyltin (IV) complexes have the ability to induce cytotoxicity in the Glioblastoma cancer cell line in lower mg/ml concentrations. In conclusion, these findings may introduce a new view on the mode of action and possible application of new compounds in the cancer chemotherapy.
Keywords: Cancer, Schiff bases, Dimethyltin (IV) Complexes, U-87
Cancer
Schiff bases
Dimethyltin (IV) Complexes
U-87
2017
6
01
113
118
http://tmuj.iautmu.ac.ir/article-1-1251-en.pdf
54-1252
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Detection of cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic resistance pattern
Mohammad Reza
Najafi
Mehdi
Parviz
dr_parviz89@yahoo.com
Kumarss
Amini
Background: Gastroenteritis due to Salmonella is common in human and considered as a global dilemma of public health. This study was done to determine cmlA/tetR, bla PSE-1, bla TEM and sip B in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile.
Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for determination of these target genes by the specific oligonucleotides primers.
Results: Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of Salmonella spp., isolates carried cml/tetR and sipB genes, respectively.
Conclusion: According to our results, detection and genotyping of virulence genes and comparison with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial purposes.
Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR
cmlA/tetR
PSE-1
TEM
sip B
Salmonella
Multiplex-PCR
2017
6
01
119
125
http://tmuj.iautmu.ac.ir/article-1-1252-en.pdf
54-1253
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Isolation and assessment of the pigments from the psychrotrophic bacteria with the aim of application in the food industries
Hadiseh
Pazoki
Parastoo
Pourali
Parastoo_pourali@yahoo.com
Background: Nowadays, the use of the organic pigments produced by the microorganisms according to their nature and safety is increasing. These pigments are various in their nature and have different properties. The aim of the recent research was to extract the new bacterial pigments that are produced in the cold environments.
Materials and methods: Ten soil samples from different areas of Torghabeh city were collected and cultivated. Ten days after, 14 pigmented bacterial colonies were selected then the isolates were identified using the biochemical tests and polymerase chain reaction (PCR). Using the methanol and glass powder, bacterial pigments were extracted and in order to purification TLC was performed. Pigments of two strains, A8 and A13, which had a suitable purity, were used for cell toxicity using three cell lines HepG2, human embryonic fibroblasts, and gastric cancer.
Results: Among 14 pigmented bacterial strains, the strain A8 that was Aeromonas hydrophyla, could prevent the growth of the cells on different cancerous cell line. Also, the pigment that was named A13, had no cytotoxic effects on all the used cell types and can be used as a natural coloring agent in the food industry.
Conclusion: As the results indicate based on the biological properties of the extracted bacterial pigments, they can be used in different areas such as medicine and technology.
Keywords: Psychrotrophic bacteria, Pigments, Food industry, Anti cancer effect.
Psychrotrophic bacteria
Pigments
Food industry
Anti cancer effect.
2017
6
01
126
132
http://tmuj.iautmu.ac.ir/article-1-1253-en.pdf
54-1254
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Alterations of gyrA in Enterobacteriaceae isolated of urinary tract infection resistance to quinolones antibiotics
Atousa
Monavari
Fatemeh
Noorbakhsh
niloofar_noorbakhsh@yahoo.com
Roozbeh
Yalfani
Background: Urinary tract infection is one of the most common infections, which if not treated, it can cause serious problems in patients. One of the ways to treat of this infection is antibiotic therapy. Nowaday, antibiotic resistance in microorganisms is a main problem for physicians and patients in the world. The aim of this study was to evaluate the genetic resistance to quinolones antibiotics in Enterobacteriaceae isolated in urine samples.
Materials and methods: 100 bacteria of Enterobacteriaceae family were isolated from suspected samples of urinary infection. Antibiotic susceptibility of isolated bacteria to quinolone antibiotics, including ciprofloxacin, nalidixic acid, norfloxacin, ofloxacin, levofloxacin and enrofloaxin, was performed by disc diffusion method according to standard guidelines (CLSI 2014). PCR was performed by specific primers of gyrA gene.
Results: Hundred bacteria were isolated of clinical urine sample including 60 E.coli, 32 Klebsiella, 3 Enterobacter, and 5 Proteus. Antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, norfloxacin 38%, ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. Totally, 36 bacteria were resist to all antibiotics, which 29 bacteria (80.55%) revealed mutation in gyrA gene.
Conclusion: This study revealed that Ecoli isolates carry a mutation in gyrA genes. This mutation has an important role in antibiotic resistance to quinolons.
Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR.
Antibiotics resistance
Quinolone
gyrA gene
Enterobacteracae
PCR.
2017
6
01
133
137
http://tmuj.iautmu.ac.ir/article-1-1254-en.pdf
54-1255
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Molecular survey of the frequency of sea and seb genes in Staphylococcus aureus isolated from skin infections in Razi hospital of Tehran
Katayoon
Abolghasemi
Naser
Harzandi
Naser.harzandi@kiau.ac.ir
Mehrooz
Dezfulian
Background: Staphylococcus aureus is one of the most common nosocomial and community acquired pathogens. The bacterium has different virulence factors and provides aggressive conditions to the host with secretion of the toxins such as super antigenic enterotoxins. Staphylococcal enterotoxin A and B (SEA,SEB) have the most severe toxic effect among these toxins. The aim of this study was to determine the frequency of A and B enterotoxin genes in Staphylococcus aureus isolated from skin infections in Tehran Razi hospital.
Materials and methods: A total of 65 isolates of Staphylococcus aureus from skin samples were collected and confirmed with phenotypic methods and checked by Polymerase Chain Reaction (PCR) using species specific primers. Then the frequency of the sea and seb genes were detected with PCR using specific primers.
Results: The PCR results showed that 86.20 % of Staphylococcus aureus isolates carried the sea gene and the frequency of seb gene in the tested isolates was 15.40 %.
Conclusion: According to the importance of Staphylococcus aureus enterotoxins and their role in the development and exacerbation of the staphylococcal diseases, the presence and expression of the corresponding genes in clinical isolates must be considered in management of the diseases.
Keywords: Staphylococcus aureus, Enterotoxins A and B, Skin infections.
Staphylococcus aureus
Enterotoxins A and B
Skin infections.
2017
6
01
138
143
http://tmuj.iautmu.ac.ir/article-1-1255-en.pdf
54-1256
2024-03-29
10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
MEDICAL SCIENCES
1023-5922
2008-3386
10.61186/iau
2017
27
2
Consideration of empowering nurses and its relationship with organizational culture and patient’s satisfaction of nursing cares
Elham
Ataee zade
Mohammad
Sahebalzamani
m_szamani@yahoo.com
Hojjatollah
Farahani
Background: Structural barriers have faced nurses with conflict of roles and feeling of powerlessness. The aim of this study was to show the importance of the empowerment of nurses in care affairs, and also corporate culture was examined as an influential factor.
Materials and methods: This descriptive – correlation study was done at hospitals dependent on Ahwaz University of Medical Sciences through quota sampling method on 400 nurses and 400 patients in 2014. Instruments for gathering data were included Work Effectiveness Questionnaire II for measuring the nurses empowering, Robbin's Organizational Culture Questionnaire and also Laschinger’s Patient Satisfaction Questionnaire.
Results: There was a significant correlation and relationship between enablement with organizational culture level (r=0.831, p<0.0001). As the corporate culture rose, the empowerment level also increased significantly and the empowerment level had a significant impact on patients' satisfaction, furthermore, the indicators effective on empowerment and satisfaction and organizational culture were identified.
Conclusion The satisfaction of patients is influenced by caregivers and health environment (dominant culture).
Keywords: Organizational culture, Empowerment, Patient satisfaction, Nurses, Iran.
Organizational culture
Empowerment
Patient satisfaction
Nurses
Iran.
2017
6
01
144
148
http://tmuj.iautmu.ac.ir/article-1-1256-en.pdf