2024-03-29T05:46:50+03:30 http://tmuj.iautmu.ac.ir/browse.php?mag_id=54&slc_lang=fa&sid=1
54-1247 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Assessment of factors affecting kidney transplant survival based on ‎literature review between 2000 and 2014‎ Reza Rabiei Hamid Moghaddasi Ali Akbar Khadem Maboudi Abbas Basiri Shahabedin Rahmatizadeh sh.rahmatizadeh@sbmu.ac.ir Background: Improvements in immune-suppressing drugs have a short-term effect on kidney ‎transplants but do not have much long-term effect. In order to increase the duration of kidney ‎transplant survival, understanding the significant parameters is of great importance. This study aims to ‎identify key parameters (before the transplant occurs) which affect kidney transplant survival. ‎ Materials and methods: To conduct this review, the scientific databases was searched and more ‎than 200 related titles were retrieved; and the abstracts were reviewed to determine which studies ‎were relevant to the study. The literature analysis was based on the goals of the studies themselves, ‎the number of citations, and the reliability of the sources cited. Ultimately, 60 studies included for ‎further analysis. ‎ Results: Based on the frequency of each parameter which influenced graft survival, the following ‎parameters were determined to have higher frequencies compared to other parameters, with regards to ‎survival time of the kidney transplant: the ages of the donor and recipient, socioeconomic status, ‎immune-suppressing drugs, HLA matching, time on the waiting list, live or deceased donor, the decade ‎in which the operation occurred, cold ischemia time, and reaction to antibiotics. ‎ Conclusion: The influence of some of the kidney transplant survival parameters was acknowledged in ‎the majority of studies; however, some parameters have been the subject of disagreement among ‎studies. In order to resolve this issue, we suggest a search of international data and meta-analysis of ‎current literature to better understand the parameters of kidney transplant survival.‎ Keywords: Kidney transplant, Kidney transplant survival, Factors affecting kidney transplant ‎survival.‎ Kidney transplant Kidney transplant survival Factors affecting kidney transplant survival.‎ 2017 6 01 77 87 http://tmuj.iautmu.ac.ir/article-1-1247-en.pdf
54-1248 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 The protective effect of ethanol extract of ginger (Zingiber ‎officinale) on spermatogenesis toxicity induced by doxorubicin‎ Fatemeh Juibar Mokhtar Mokhtari M.Mokhtari246@yahoo.com Mehdi Dianatpur Mehrdad Shariatie Background: DOX is one of the most common drugs used to treat different types of cancer, including ‎testicular cancer. The aim of this study was to evaluate the effect of ethanol extract of ginger on ‎spermatogenesis in rats. ‎ Materials and methods: In this experimental study, 56 male rats Wistar were randomly divided into 6 ‎groups of 8 animals control, the sham and experimental group 1 receiving doxorubicin (mg/kg.bw 3) ‎experimental 2 and 3 receiving ginger extract (500 , 1000 mg/ kg. bw ) and experimental 4 and 5 ‎received a ginger extract (500 , 1000 mg/ kg. bw ) + doxorubicin. The rats were fed extract daily, one ‎hour after doxorubicin injection, for four weeks. DOX (3mg/kg) was administered once a week ‎intraperitoneally. Some sperm parameters, such as the number, viability, morphology and mobility were ‎examined using Eosin-Ngrozin staining. ‎ Results: A significant decrease in body weight, sperm count, sperm motility, viability was observed in ‎drug-DOX-treated rats compared with the control group. The reduction in the group treated with DOX ‎with ginger 500 and 1000 mg/ kg significantly was offset compared with DOX. ‎ Conclusion: Ginger is able to have protective effect on sperm parameters in adult male rat induced by ‎Doxorobicin.‎ Keywords: Ginger, Doxorubicin, Body weight, Testes, Sperm parameters, Rat.‎ Ginger Doxorubicin Body weight Testes Sperm parameters Rat.‎ 2017 6 01 88 96 http://tmuj.iautmu.ac.ir/article-1-1248-en.pdf
54-1249 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 The evaluation of hippocampal level of brain derived neurotrophic ‎factor and spatial memory in valproic acid animal model of autism Zahra Borzu Mohammad Amin Edalatmanesh amin.edalatmanesh@gmail.com Background: Autism, a behavior-developmental disorder, is characterized by impairments in social ‎interaction, communications, as well as restricted, repetitive, and stereotyped patterns of behavior. The ‎studies revealed changes in hippocampal level of brain-derived neurotrophic factor (BDNF) in autism ‎etiology. However, their level and mechanism of action are needed to be elucidated. This study aimed ‎to determine the hippocampal levels of BDNF and its relation with spatial memory in valproic acid ‎animal model of autism. ‎ Materials and methods:‎‌ ‌In this experimental study, 40 male and female Sprague Dawley rat pups ‎were divided into two Phosphate-Buffered Saline receiver (PBS, n=20) and Valproic Acid receiver ‎‎(VPA, n=20) groups. VPA model of autism was induced by intra-peritoneal administration of VPA ‎‎(500 mg/kg) at 12.5 days after gestation. To measure changes in spatial memory Morris water maze ‎test was used. Then, the hippocampal levels of BDNF were determined by ELISA method. ‎ Results: Increased spatial memory was observed in Morris water maze test among VPA group ‎compared to PBS group. In addition, the hippocampal levels of BDNF in VPA rats were significantly ‎higher than PBS group (p<0.05). ‎ Conclusion: Increasing BDNF showed enhancement of spatial memory in VPA rat model of autism‎‌.‌ Keywords: Autism, Valproic acid, BDNF, Spatial memory.‎ Autism Valproic acid BDNF Spatial memory 2017 6 01 97 104 http://tmuj.iautmu.ac.ir/article-1-1249-en.pdf
54-1250 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Evaluation of serum angiotensin converting enzyme (ACE) activity ‎in type 1 Gaucher’s patient and its relationship to ACE gene ‎insertion/deletion in intron 16‎ Morteza Ghazanfari Jajin Shohreh Khatami Hadi Mozafari Abolfazl Fateh Maryam Mobaraki Mohammad Taghikhani Background: Gaucher's disease is an autosomal recessive disease which is the result of mutations in ‎the β glucocerebrosidase gene. The aim of this study was to evaluate activity level of ACE enzyme ‎Iranian patients with Gaucher’s disease type I, and also polymorphism I/D in intron 16‎‌ ‌of ACE gene, as ‎a marker in diagnosis and monitoring of disease. ‎ Materials and methods:‎‌ ‌The experiments were performed on 29 patients (mean age of 10.04 years) ‎and 60 healthy subjects (mean age of 7.31 years). Procedures included DNA extraction from blood, ‎detection of polymorphism I/D by PCR and evaluation of activity level of ACE enzyme.‎ Results:‎‌ ‌The mean of ACE activity was 231.07 U/L which was increased 4 times than normal status ‎‎(56.03 U/L). Evaluation of polymorphism I/D of the 29 patients showed t6 (20.7%) II, 9 (31%) DD and ‎‎14 (48.3%) ID (p<0.05).‎ Conclusion: According to the results, the measurement of the ACE activity levels can be used as ‎cofactors in diagnosis and as well as an important factor in the monitoring of treatment. Polymorphism ‎I/D with respect to the role of the ACE activity can be effective in increasing the specificity of the ‎experiments.‎ Keywords: Gaucher's disease, Angiotensin converting enzyme (ACE), β- glucocerebrosidase, ‎Polymorphism I/D.‎ Gaucher's disease Angiotensin converting enzyme (ACE) β- glucocerebrosidase ‎Polymorphism I/D.‎ 2017 6 01 105 112 http://tmuj.iautmu.ac.ir/article-1-1250-en.pdf
54-1251 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Anticancer effects of some Girard-P reagent-based schiff bases and ‎their Dimethyltin (IV) complexes on malignant glioblastoma cell line ‎in in vitro culture Elham hoveizi e.hoveizi@yahoo.com Zynab Ansariasl Background: Glioblastoma (U-87) is the most common and most malignant of the glial tumors that ‎appeared in the central nervous system. This is the first study that has evaluated the cytotoxic effects ‎of various Girard-P reagent-based Schiff bases and their dimethyltin (IV) complexes on Glioblastoma ‎cell line, U-87, viability. ‎ Materials and methods: In this experimental study, U-87 cell line was grown in DMEM ‎supplemented with 10% FBS, penicillin/streptomycin (100 U/ml, 100 µg/ml) at 37 ◦C in 5% CO2, then ‎the effects of different concentrations of complexes on Glioblastoma cell were evaluated by MTT and ‎DAPI staining. ‎ Results: Herein, we demonstrated that these compounds had cytotoxicity effects of Glioblastoma ‎cancer cells. In a dose dependent manner, a significant cytotoxicity was observed with increasing of ‎P1M and P3. ‎ Conclusion: The results showed that Girard-P reagent-based Schiff bases and their dimethyltin (IV) ‎complexes have the ability to induce cytotoxicity in the Glioblastoma cancer cell line in lower mg/ml ‎concentrations. In conclusion, these findings may introduce a new view on the mode of action and ‎possible application of new compounds in the cancer chemotherapy.‎ Keywords: Cancer, Schiff bases, Dimethyltin (IV) Complexes, U-87‎ Cancer Schiff bases Dimethyltin (IV) Complexes U-87‎ 2017 6 01 113 118 http://tmuj.iautmu.ac.ir/article-1-1251-en.pdf
54-1252 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Detection of ‎‌‎ cmlA/tetR, ‎‌bla PSE-1, bla TEM and sip B in the ‎Salmonella strains by Multiplex-PCR method and their antibiotic ‎resistance pattern Mohammad Reza ‎ Najafi‎ Mehdi Parviz dr_parviz89@yahoo.com Kumarss‎ Amini‎ Background: Gastroenteritis due to Salmonella is common in human and considered as a global ‎dilemma of public health. This study was done to determine cmlA/tetR, ‎‌bla PSE-1, bla TEM and sip B ‎in the Salmonella strains by Multiplex-PCR method and their antibiotic susceptibility profile. ‎ Materials and methods: In this cross-sectional study, 163 clinical samples were obtained from ‎patients admitted to Hazrat-e Rasool General Hospital. The antibiotic susceptibility test was determined ‎using the disk diffusion method agreeing with CLSI guideline. Then, M-PCR was achieved for ‎determination of these target genes by the specific oligonucleotides primers. ‎ Results: Of 163 collected samples, 48(29.4%) Salmonella spp., were obtained, which 25(52.1%) were ‎S. enteritidis, 14(29.2%) S. typhimurium and 9(18.7%) S. infantis. Antibiotic resistance analysis showed ‎that the highest resistance rate were related to tetracycline (n: 27, 56.2%) and then streptomycin and ‎chloramphenicol (n: 15, 31.2%). All isolates (n: 48, 100%) were susceptible to imipenem, amikacin, ‎gentamycin and trimethoprim/sulfamethoxazole. The MPCR results revealed that 62.5% and 16.6% of ‎Salmonella spp., isolates carried cml/tetR and sipB genes, respectively. ‎ Conclusion: According to our results, detection and genotyping of virulence genes and comparison ‎with global ranging is a basic requirement in the control and prevention of salmonellosis in industrial ‎purposes.‎ Keywords: cmlA/tetR, PSE-1, TEM, sip B, Salmonella, Multiplex-PCR cmlA/tetR PSE-1 TEM sip B Salmonella Multiplex-PCR 2017 6 01 119 125 http://tmuj.iautmu.ac.ir/article-1-1252-en.pdf
54-1253 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Isolation and assessment of the pigments from the psychrotrophic‌ ‌bacteria with the aim of application in the food industries ‎ Hadiseh Pazoki Parastoo Pourali Parastoo_pourali@yahoo.com Background: Nowadays, the use of the organic pigments produced by the microorganisms according ‎to their nature and safety is increasing. These pigments are various in their nature and have different ‎properties. The aim of the recent research was to extract the new bacterial pigments that are produced ‎in the cold environments. ‎ Materials and methods: Ten soil samples from different areas of Torghabeh city were collected and ‎cultivated. Ten days after, 14 pigmented bacterial colonies were selected then the isolates were ‎identified using the biochemical tests and polymerase chain reaction (PCR). Using the methanol and ‎glass powder, bacterial pigments were extracted and in order to purification TLC was performed. ‎Pigments of two strains, A8 and A13, which had a suitable purity, were used for cell toxicity using ‎three cell lines HepG2, human embryonic fibroblasts, and gastric cancer. ‎ Results: Among 14 pigmented bacterial strains, the strain A8 that was Aeromonas hydrophyla, could ‎prevent the growth of the cells on different cancerous cell line. Also, the pigment that was named A13, ‎had no cytotoxic effects on all the used cell types and can be used as a natural coloring agent in the ‎food industry. ‎ Conclusion: As the results indicate based on the biological properties of the extracted bacterial ‎pigments, they can be used in different areas such as medicine and technology.‎ Keywords: Psychrotrophic bacteria, Pigments, Food industry, Anti cancer effect.‎ Psychrotrophic bacteria Pigments Food industry Anti cancer effect.‎ 2017 6 01 126 132 http://tmuj.iautmu.ac.ir/article-1-1253-en.pdf
54-1254 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Alterations of gyrA in Enterobacteriaceae isolated of urinary tract ‎infection resistance to quinolones antibiotics Atousa Monavari Fatemeh Noorbakhsh niloofar_noorbakhsh@yahoo.com Roozbeh Yalfani Background: Urinary tract infection is one of the most common infections, which if not treated, it can ‎cause serious problems in patients. One of the ways to treat of this infection is antibiotic therapy. ‎Nowaday, antibiotic resistance in microorganisms is a main problem for physicians and patients in the ‎world. The aim of this study was to evaluate the genetic resistance to quinolones antibiotics in ‎Enterobacteriaceae isolated in urine samples. ‎ Materials and methods: 100 bacteria of Enterobacteriaceae family were isolated from suspected ‎samples of urinary infection. Antibiotic susceptibility of isolated bacteria to quinolone antibiotics, ‎including ciprofloxacin, nalidixic acid, norfloxacin, ofloxacin, levofloxacin and enrofloaxin, was ‎performed by disc diffusion method according to standard guidelines (CLSI 2014). PCR was ‎performed by specific primers of gyrA gene.‎ Results: Hundred bacteria were isolated of clinical urine sample including 60 E.coli, 32 Klebsiella, 3 ‎Enterobacter, and 5 Proteus. Antibiotic resistance to ciprofloxacin were 36%, nalidixic acid 45%, ‎norfloxacin 38%, ofloxacin 38%, levofloxacin 35% and enrofloaxin 39%. Totally, 36 bacteria were ‎resist to all antibiotics, which 29 bacteria (80.55%) revealed mutation in gyrA gene. ‎ Conclusion: This study revealed that Ecoli isolates carry a mutation in gyrA genes. This mutation has ‎an important role in antibiotic resistance to quinolons.‎ Keywords: Antibiotics resistance, Quinolone, gyrA gene, Enterobacteracae, PCR.‎ Antibiotics resistance Quinolone gyrA gene Enterobacteracae PCR.‎ 2017 6 01 133 137 http://tmuj.iautmu.ac.ir/article-1-1254-en.pdf
54-1255 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Molecular survey of the frequency of sea and seb genes in ‎Staphylococcus aureus isolated from skin infections in Razi hospital ‎of Tehran Katayoon Abolghasemi Naser Harzandi Naser.harzandi@kiau.ac.ir Mehrooz Dezfulian Background: Staphylococcus aureus is one of the most common nosocomial and community ‎acquired pathogens. The bacterium has different virulence factors and provides aggressive conditions ‎to the host with secretion of the toxins such as super antigenic enterotoxins. Staphylococcal enterotoxin ‎A and B (SEA,SEB) have the most severe toxic effect among these toxins. The aim of this study was ‎to determine the frequency of A and B enterotoxin genes in Staphylococcus aureus isolated from skin ‎infections in Tehran Razi hospital. ‎ Materials and methods: A total of 65 isolates of Staphylococcus aureus from skin samples were ‎collected and confirmed with phenotypic methods and checked by Polymerase Chain Reaction (PCR) ‎using species specific primers. Then the frequency of the sea and seb genes were detected with PCR ‎using specific primers. ‎ Results: The PCR results showed that 86.20 % of Staphylococcus aureus isolates carried the sea ‎gene and the frequency of seb gene in the tested isolates was 15.40 %. ‎ Conclusion: According to the importance of Staphylococcus aureus enterotoxins and their role in the ‎development and exacerbation of the staphylococcal diseases, the presence and expression of the ‎corresponding genes in clinical isolates must be considered in management of the diseases.‎ Keywords: Staphylococcus aureus, Enterotoxins A and B, Skin infections.‎ Staphylococcus aureus Enterotoxins A and B Skin infections.‎ 2017 6 01 138 143 http://tmuj.iautmu.ac.ir/article-1-1255-en.pdf
54-1256 2024-03-29 10.1002
Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61186/iau 2017 27 2 Consideration of empowering nurses and its relationship with ‎organizational culture and patient’s satisfaction of nursing cares Elham‎ Ataee zade ‎ Mohammad Sahebalzamani m_szamani@yahoo.com Hojjatollah Farahani Background: Structural barriers have faced nurses with conflict of roles and feeling of powerlessness. ‎The aim of this study was to show the importance of the empowerment of nurses in care affairs, and ‎also corporate culture was examined as an influential factor. ‎ Materials and methods: This descriptive – correlation study was done at hospitals dependent on ‎Ahwaz University of Medical Sciences through quota sampling method on 400 nurses and 400 patients ‎in 2014. Instruments for gathering data were included Work Effectiveness Questionnaire II for ‎measuring the nurses empowering, Robbin's Organizational Culture Questionnaire and also ‎Laschinger’s Patient Satisfaction Questionnaire. ‎ Results: There was a significant correlation and relationship between enablement with organizational ‎culture level (r=0.831, p<0.0001). As the corporate culture rose, the empowerment level also increased ‎significantly and the empowerment level had a significant impact on patients' satisfaction, furthermore, ‎the indicators effective on empowerment and satisfaction and organizational culture were identified.‎ Conclusion The satisfaction of patients is influenced by caregivers and health environment (dominant ‎culture).‎ Keywords: Organizational culture, Empowerment, Patient satisfaction, Nurses, Iran.‎ Organizational culture Empowerment Patient satisfaction Nurses Iran.‎ 2017 6 01 144 148 http://tmuj.iautmu.ac.ir/article-1-1256-en.pdf