2026-05-08T15:30:57+03:30 http://tmuj.iautmu.ac.ir/browse.php?mag_id=75&slc_lang=fa&sid=1

75-2216 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Fatemeh Ganjali Mohammad Rostami-Nejad , Hamid Asadzadeh Aghdaei Mehrdad Hashemi mhashemi@iautmu.ac.ir Background and Aim: Based on previous studies, T-bet can be effective in inflammation and worsening of celiac disease by improving the progression of Th1-mediated responses and the production of pro inflammatory cytokines such as interferon-gamma (IFNγ) and tumor necrosis factor-alpha (TNF-α). The aim of this study was to evaluate the expression of T-bet gene in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals. Methods: In this study, 20 peripheral blood samples were collected from patients (37.5±16.07) with celiac disease and 20 samples from healthy individuals (36.47±8.62) as a control group. After RNA extraction and cDNA synthesis, a specific primer pair of T-bet gene was designed and used after confirmation with blast software. PCR was performed and then T-bet gene expression was assessed by real-time PCR. Results: In this study, 12 (60%) females and 8 (40%) males in the patient group and 13 (65%) females and 7 (35%) males in the control group were studied. T-bet gene expression was not significantly different in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals (Pvalue: 0.27). Conclusion: The results of quantitative analysis of the expression of this gene in patients with celiac disease treated with gluten-free diet (for 6 months or more) compared with controls showed that this gene cannot be used as a diagnostic biomarker to differentiate Patients from healthy individuals.   Celiac Disease T-bet transcription factor Gluten Free Diet DNA Primer PCR 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2216-en.pdf

75-2064 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Maryam Ghanbarirad Mehrdad Hashemi drmehashemi@gmail.com Seyed Mehdi Saberi Ahmad Majd Background & objectives: Major depressive disorder and bipolar disorder are among the most common psychiatric disorders worldwide, and put a heavy burden on the society. Major depressive disorder’s symptoms include depressed mood, loss of interest, weight lose/gain, etc. Bipolar disorder manifest as two distinct episodes, including mania/ hypomania which consists of irregular elevated mood and depression. These disorders are polygenic in nature, and multiple investigations have reported various genes with small impacts to be associated with these conditions. NRF2 as a member of antioxidant system participates in oxidative stress detoxifying pathway, and MYT1 is involved in neurogenesis. Several studies have reported these transcription factors to play a role in Notch signaling pathway, and therefore they may interact with one another. In this study the expression level of these transcription factors and their potential correlation has been investigated. Material & methods: Real time PCR was conducted to assess the expression level of NRF2 and MYT1in the peripheral blood of major depressive disorder and bipolar disorder patients in comparison with healthy individuals. In addition, Wechsler subtests were used to evaluate working memory function in individuals. Results: Results demonstrate significant downregulation of NRF2 and MYT1. Pearson correlation analysis revealed positive correlation between these two transcription factors. In addition, ROC curve analysis indicated the peripheral biomarker characteristic of NRF2 and MYT1. Data suggests a reduction in the working memory functions of affected individuals in comparison with healthy subjects. Conclusion: Biomarker characteristics of NRF2 and MYT1 illustrates their potential to be used as prognostic and diagnostic factors for these conditions. Furthermore, despite of having distinct roles in cells, observed positive correlation might be due to an upstream regulator and a reveal a mutual signaling pathway for NRF2 and MYT1. More studies are needed to investigate all the possible common pathways between NRF2 and MYT1. In addition, larger samples are necessary to improve the statistical power of the analysis.   Major depressive disorder Bipolar disorder Neurogenesis Inflammation Realtime PCR 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2064-en.pdf

75-2063 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Hanieh Voshmgiri Fereshteh Atabi f.atabi@iautmu.ac.ir Malihe Entezari Malak Hekmati ‌Background & objectives: F. vulgare fennel seeds are rich in various secondary (phytochemical) plant metabolites such as polyphenolic acids, flavonoids and saponins. Materials & methods: After extracting fennel seeds using a balloon shuffle, the resulting extract containing anethole was filtered. In a round bottom flask, 0.5 g of gold salt was added to 250 ml of deionized water at boiling point. Results: Black precipitate was obtained from ruby ​​nanoparticle solution. SEM, TEM and nanoparticle sizes less than 20 nm were obtained. FTIR proved the presence of metal particles. The scattering of gold nanoparticles using map analysis of the constituent elements using EDX was appropriately confirmed. Conclusion: The results of FTIR, SEM, TEM, EDX and map analysis confirmation tests confirm the size and shape of nanoparticles synthesized in this study. By examining its effect on cancer cells, we can also evaluate their quality and mortality.   Gold nanoparticles fennel anethole 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2063-en.pdf

75-2062 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Masoumeh Masoumi masomeh_masomi@yahoo.com Mitra Salehi Seyed Abdolhamid Angaji Mehrdad Hashemi mhashemi@iautmu.ac.ir Background and Aim: Testicular torsion is a urological emergency that leads to testicular tissue damage and infertility. In recent years, several studies have been conducted on the antioxidant properties of nanoparticles. The aim of this study was to investigate the effects of Diamond nanoparticles on the  apoptosis in rats with testicular ischemia / reperfusion. Methods: 48 adult rats aged 20 weeks and weighing 250 g in the control and ischemia / testicular reperfusion groups were included in the study. Both groups were treated with q10 and nanodiamond at a dose of LD50 for 10 days. Then the testes were removed and the survival and apoptosis of the cells were assessed by MTT and flowcytometry and the expression of BAX and BCL2 genes was measured. Results: Treatment with nanodiamond in the control group increased the expression of BAX gene and decreased the expression of BCL2 gene, while in the ischemia / reperfusion group it had the opposite effect. Simultaneous use of Q10 and nanodiamond compensated for the adverse effects of nanodiamond (P <0.05). Conclusion: Nanodiamond at LD50 dose in rats with testicular ischemia / reperfusion had antioxidant effects and reduced apoptosis.   Diamond nanoparticles antioxidant ischemia / reperfusion apoptosis 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2062-en.pdf

75-2061 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Haniyeh Motie Arani Fereshteh Atabi f.atabi@iautmu.ac.ir Saeed Hesami Tackallou tackallou@gmail.com Hakimeh Zali Minoo Shahani ‌Background & objectives:  Breast cancer is one of the most common diseases in women around the world and has many causes. Calcium regulation plays an essential role in cancer cell tumorigenesis and cell proliferation, migration, metastasis and resistance to apoptosis. Nanocalcium inhibits tumor by changing the pH of cancer cells. In this study, calcium nanofluoride was synthesized and evaluated as one of the treatment candidates and reducing symptoms in breast cancer.   Materials & methods: The mixture of calcium carbonate was sonicated with hydrogen fluoride and the nanoparticles were confirmed by DLS and TEM techniques. MCF-7, MDA-MB-231, MDA-MB-468, BT-474 and healthy MSC cancer cells were cultured. MTT test was also performed to determine the IC50 of the drug and to perform tests for apoptosis, cell cycle, abrasion, hemorrhage, invasion and colonization. Results: The IC50 level of calcium nanofluoride treatment was 1.2 mg / ml using MTT test, but 0.6 mg / ml was used for apoptosis and cell cycle tests with an apoptosis rate of 2.8% in MDA cells. MB-23117% in MCF-7 cells and 4% in BT cells showed no significant extent. Also, MDA-MB-231 cell cycle in G1 and G2 stages showed an increase of 6.7% and 1.01%, respectively, and in S stage showed a decrease of 5.79% without significant extent. MCF-7 decreased by 11.46 and 6.43 percent in G1 and G2 stages, respectively, and increased by 22.52 percent in S stage. BT in stages G1 and G2 shows a 64% increase. Conclusion: Calcium nanofluoride increased cell death by initiating apoptosis, showed good performance in inhibiting and reducing colonization, closure of the scratch site, invasion, sphericity of breast cancer cells of MDA-MB-231 cell line.   Breast cancer MDA-MB231 cell line Calcium nanofluoride 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2061-en.pdf

75-2060 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Zahra Salehi-Shafa Elham Rajabbeigi Leila Youseftabar miri rajabbeigi@iautmu.ac.ir Background: Liver cancer is the second leading cause of cancer deaths in the world and is therefore a major public health challenge. In recent years, many of the drugs used in the treatment of many malignancies and cancers have been of plant origin. Lavender extract has been shown to have therapeutic effects in various cancers. Therefore, the biological effects of ethanolic extract of lavender on liver cancer cells were investigated Methods: In this experimental study, HepG2 cell line was selected for treatment with lavender extract (5, 10, 20, 30, 40 and 50 mg/ml). Cell proliferation in the presence of different concentrations of lavender extract was studied by trypan blue staining. Also, the effects of lavender on apoptosis and necrosis were investigated using flow cytometry method. Finally, the antioxidant activity was evaluated using the DPPH method. Results: The results of cell proliferation studies showed that with increased time and concentration of the extract, the rate of cell proliferation decreases. Flow cytometry findings showed that lavender extract had an apoptotic effect on cancer cells. In addition, the DPPH test showed that increasing the concentration of lavender plant extract reduced free radical control and increased antioxidant activity. Conclusion: According to the results of this study, it can be stated that lavender extract can have significant inhibitory effects on the proliferation of HepG2 cancer cells. Hence, the use of safer therapies with less side effects, such as the use of lavender, is important in inhibiting cancer cells, particularly liver cancer   Apoptosis Antioxidant Free Radical Lavernder Liver Cancer 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2060-en.pdf

75-2059 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Shadi Sarahroodi Sarahroodi@yahoo.com Usually, the elderly and patients with chronic diseases visit several doctors and are supposed to take multiple medications. The multiplicity of medications is called polypharmacy and it may cause side effects, drug interactions and other helth issues for the patient, and it might go as far as the patient might need to go to the hospital and be admitted in ICU, or even further and be the cause of death for the patient. Experts have determined that if a person uses four medications, the fifth one will have the equal chance of risk and benefit for him/her. The constant use of more than 5 drugs is called polypharmacy and its prevalence varies in different societies and various age groups. The phenomenon of polypharmacy is common in elderly and patients with chronic diseases. Since it can be potentially dangerous and it is growing fast globally. Researchers around the world are trying to establish methods to deal with this issue and improve the situation by decreasing the number of un needed medications for their patients. This method is called “Deprescribing” and there is an urgent need that Doctors, pharmacists and other health care professionals get familiar and join this process to serve patients better and and prevent the complications caused by polypharmacy as much as possible.   polypharmacy Deprescribing Drug intraction Side effects polyprescription 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2059-en.pdf

75-2058 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Shabnam Zarei Moradi Seyed Abdolhamid Angaji mitra salehi mehrdad hashemi mhashemi@iautmu.ac.ir Background and Aims: Testicular ischemia / reperfusion is one of the most serious urological emergencies that is generally identified in men, adolescents and infants. This causes damage to the testicles which, if misdiagnosed and treated improperly, can lead to infertility in men. In the present study, the effects of curcumin and Fe3O4 nanoparticles on the rate of apoptosis and expression of BAD, BCL-X genes were studied. Materials and Methods: 48 adult rats aged 20 weeks and weighing 250 g in the control and ischemia / reperfusion (I / R) groups were included in the study. After induction of testicular torsion in the experimental group, both groups were treated with curcumin and iron nanoparticles at a dose of LD50 for 10 days and subcutaneously. Then the testes were removed and the survival and apoptosis of the cells were assessed by MTT and flow cytometry and the expression of BAD and BCLX genes was measured. Results: Increased apoptosis was observed in testicular tissue cells of rats with ischemia / reperfusion injury. Exposure to iron nanoparticles increased BAD proapoptotic gene expression and decreased BCLX anti-apoptotic gene expression. Co-administration of curcumin and iron nanoparticles offset the adverse effects of nanoparticles. (P <0.05) Discussion and Conclusion: It seems that co-administration of Fe3O4 nanoparticles with curcumin is effective in reducing the damage caused by testicular torsion due to its antioxidant properties and reduces necrosis. The synthesis of green nanoparticles can also be considered.   BAD BCLX testicular torsion gene expression apoptosis iron nanoparticles 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2058-en.pdf

75-2057 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Faramarz Khosravi Vida Hojati Malihe Entezari Mentezari@iautmu.ac.ir Mehrdad Hashemi ‌Background & objectives: Parkinson's disease is one of the most common neurodegenerative diseases that its prevalence has increased in recent years. Although many efforts have been made to treat this disease, so far, no therapeutic approach has been found that can stop the destruction of dopaminergic cells in the substantia nigra. The aim of the present study was to investigate the effect of vitamin B12 administration on Parkinson's female mice and their embryos. Materials & Methods: Induction of Parkinson's disease in female mice was performed using rotenone and vitamin B12 administration was started one hour after Parkinson's induction and was performed for 14 days. On day 14, the animals underwent apomorphine-induced rotation testing, and then half of the animals were killed according to ethical protocols, and cerebellar tissue homogenates were prepared. The other half of the animals were allowed to mate with male mice and kept in separate cages after their pregnancy was confirmed. The embryos were isolated on the 21st day of gestation and their cerebellar tissue homogenates were also prepared. Cell viability was measured by MTT method and apoptosis and necrosis were measured by flow cytometry. Also, the expression of bax and bcl-2 genes was measured using RT-PCR. Data analysis was performed in GraphPad Prism V.8 software. Results: Survival, apoptosis and necrosis of cerebellar neurons in female mice and fetuses from Parkinson's mice were significantly affected by vitamin B12 administration and an increase in survival and a decrease in apoptosis and necrosis of cerebellar neurons were observed with vitamin B12 administration. Also, decreased bax gene expression and increased bcl-2 expression were observed in the cerebellar neurons of Parkinson's animals receiving vitamin B12. Conclusion: Vitamin B12 has neuroprotective effects in Parkinson's disease and can be considered as a treatment option.   Parkinson's mice gene apoptosis vitamin B12 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2057-en.pdf

75-2056 2026-05-08 10.1002

Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch MEDICAL SCIENCES 1023-5922 2008-3386 10.61882/iau 2021 31 ويژه نامه - کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی و داروسازی و صنایع غذایی تهران، دی 1400 Fatemeh Khavari Hamed Manoochehri Khoshinani Massoud Saidijam Fatemeh Nouri Fatemenouri1@gmail.com اولین کنگره ملی رویکردهای نوآورانه در سی ‌Background & objectives: Colorectal cancer (CRC) is the most common gastric cancer and third lethal cancer worldwide. Despite development in diagnostic and treatment methods, due to the lack of typical symptoms in the early stages, most patients are diagnosed in the end stages. Therefore, finding simple, inexpensive, highly sensitive and specific diagnostic methods for early detection and prognosis of  colorectal cancer is important. piRNAs are one of the things that can be paid special attention to. In this review article, the function of these biomolecules in tumorigenesis are studied for diagnosis. Materials & Methods: The bibliographic search was performed on PubMed, Scopus, and Web of Science databases. Any language or date restrictions were not applied. Identified studies were screened by title, abstract, and full text. Results: piRNA expression is dysregulated in different tumor tissues such as and colorectal cancer compare with normal tissues. This regulatory RNAs role as oncogene or tumor suppressor in signaling pathways related to survival, proliferation, invasion, and metastasis of cancer cells. Some piRNAs expressions correlate with clinicopathologic features of cancer. Due to their small molecular size piRNAs can pass through cellular membrane and enter the bloodstream. Conclusions: Some piRNAs such as piR-5937, piR-28876, piR-020450 and piR-020619 elevate in serum of CRC patients as compared with healthy people and could be used as early diagnostic biomarkers with higher sensitivity and specificity compared to carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) for colorectal cancer.   Colorectal cancer Piwi-Interacting RNA Biomarker 2021 12 01 0 0 http://tmuj.iautmu.ac.ir/article-1-2056-en.pdf