fa بررسی اثر پروپوفول بر روی سلول‌های ناحیهCA2و CA3 هیپوکامپ موش صحرایی مدل ایسکمی ریپرفیوژن علوم تشريحي Anatomical Sciences تجربي Experimental <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">ایسکمی/رپرفیوژن مغزی منجر به </span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">مرگ برنامه&shy;ریزی شده سلول یا </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">آپوپتوز می&shy;شود.</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">هیپوکامپ بافتی&nbsp; بسیار حساس </span></span></span></i><i>&nbsp;</i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">به ایسکمی مغزی &nbsp;است. </span></span></span></i><i>&nbsp;</i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">پروپوفول نوعی داروی بیهوشی است که اخیرا به عنوان حفاظت کننده عصبی مورد توجه قرار گرفته است. در این تحقیق اثر پروپوفول در ناحیه </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">CA2</span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">و </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">CA3</span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">هیپوکامپ &nbsp;متعاقب ایسکمی بررسی شد</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> <i>۲۴</i></span></span></span></span><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">موش صحرایی نر ویستار به </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">۴</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">گروه </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">۶</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">تایی کنترل، ایسکمی، حامل و آزمایشی تقسیم شدند. گروه آزمایشی </span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">&nbsp;mg/kg </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">40 داروی پروپوفول </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">و </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">گروه &nbsp;حامل</span></span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">۱</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">میلی &shy;لیتر نرمال سالین </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">۱</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">ساعت قبل از ایسکمی به صورت داخل صفاقی&nbsp; دریافت کردند</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> ایسکمی با بستن شریان&shy;های کاروتید مشترک دو طرف به مدت 20 دقیقه و سپس رپرفیوژن القاء شد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">. ۴</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">روز بعد تمامی رت&shy;ها قربانی شدند و بافت هیپوکامپ به روش رنگ آمیزی نیسل بررسی شد</span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> داده&shy;ها با استفاده از نرم افزار آماری </span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">SPSS-25</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> و آزمون آماری </span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">ANOVA</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> یک طرفه و تست </span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">TUKEY</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> مورد تحلیل قرار گرفتند. سطح معنی&shy; داری 05/0</span></span></span></span></i><i><span dir="LTR" style="font-size:12.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">p<</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"> در نظر گرفته شد</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">.</span></span></span></span><i>&nbsp; </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> ایسکمی رپرفیوژن به مدت </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">۲۰</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">دقیقه باعث </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">دژنراسیون</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">سلول&shy;های هرمی ناحیه </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA2</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">و</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA3</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">هیپوکامپ شد و این نورون&shy;ها</span></span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">کا</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">هش قابل ملاحظه&shy;ای را نسبت به گروه کنترل نشان دادند، اما تزریق پروپوفول موجب مهار کاهش&nbsp; تعداد سلول&shy;های سالم در این دو ناحیه شد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">&shy;</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">پروپوفول می&shy;تواند به عنوان عاملی موثر در پیشگیری یا کاهش عوارض سکته مغزی به تنهایی و یا با سایر داروها مورد استفاده قرار گیرد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" koodak="" style="font-family:"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Cerebral ischemia/ reperfusion leads to programmed cell death or planned apoptosis. Hippocampus is a very sensitive tissue to cerebral ischemia. Propofol is an anesthesia that recently the use of this drug as a neuroprotective has been considered. In this study, the effect of propofol on CA2 and CA3 areas of the hippocampus following ischemia was investigated. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: 24 Wistar rats were randomly divided into 4 groups, including: control ischemia, experimental and vehicle. The experimental group received 40 mg/ kg of propofol and the vehicle group received 1 ml normal saline 1 hour before ischemia intraperitoneally. The ischemic model was performed by bilateral closure of the common carotid arteries for 20 minutes then reperfusion was done. 4 days later, all rats were sacrificed and the hippocampal tissue was examined by Nissl staining method. Data were analyzed using SPSS-25 statistical software by one-way ANOVA and TUKEY test. p<0.05 was considered as Significant. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: Ischemia/ reperfusion for 20 minutes caused degeneration of pyramidal cells in CA2 and CA3 hippocampus and these neurons showed a significant decrease compared to the control group, but propofol injection inhibited the decrease in the number of viable cells in these two areas.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: Propofol can be used as an effective agent in preventing or reducing the complications of stroke alone or with other drugs.</span></span></span></span></span></span><br> &nbsp; پروپوفول , هیپوکامپ, ایسکمی ریپرفیوژن, موش صحرایی Propofol, Hippocampus, Ischemia/reperfusion, Rat 389 397 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-364-2&slc_lang=fa&sid=1 Mostafa Rahchamani مصطفی راه چمنی 100319475328460010851 100319475328460010851 Yes Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran دانشکده پزشکی، دانشگاه علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Shabnam Movassaghi شبنم موثقی sm_movassaghi@yahoo.com 100319475328460010852 100319475328460010852 No Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran - Herbal Pharmacology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه علوم تشریح و علوم اعصاب شناختی، دانشکده پزشکی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران-مرکز تحقیقات فارماکولوژی گیاهان دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Zahra kermaniha زهرا کرمانیها zahra.kermaniha@gmail.com 100319475328460010853 100319475328460010853 No Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran دانشکده پزشکی، دانشگاه علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران Zahra Nadia Sharifi زهرا نادیا شریفی Zsharifi@iautmu.ac.ir 100319475328460010854 100319475328460010854 No Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran - Herbal Pharmacology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran گروه علوم تشریح و علوم اعصاب شناختی، دانشکده پزشکی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران-مرکز تحقیقات فارماکولوژی گیاهان دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران