[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 35, Issue 1 (spring 2025) ::
MEDICAL SCIENCES 2025, 35(1): 24-34 Back to browse issues page
Investigating the antioxidant properties and antimicrobial activity of green synthesis of silver nanoparticle by using the aqueous extract of Hypericum perforatum
Fahimeh Najafi1 , Zahra Shafiei2
1- Department of chemistry, School of Science and Agriculture, Roudehen Branch, Islamic Azad University, Roudehen, Iran
2- Assistant Professor ofMicrobial Biotechnology, Department of Biology,Ro.C.,Islamic Azad University,Roudehen,Iran , shafieezahra641@gmail.com
Abstract:   (654 Views)

Background: Today, antibiotic resistance against bacteria causing infection is very common and biological production of nanoparticles is a suitable alternative. In the present study, the synthesis of silver nanoparticle of Hypericum perforatum plant and its antioxidant and antibacterial properties were investigated in comparison with antibiotic gentamicin.

Materials and methods: After collecting plant and preparing the aqueous extract, 1 mM silver nitrate was added for nanoparticle synthesis. The effect of pH parameters and silver nitrate concentration for the optimization of green nanoparticle was evaluated by spectrophotometry and SEM. Antioxidant properties were investigated by DPPH and ABTS free radical methods. Finally, the antibacterial properties of the above nanoparticle were determined by diffusion method in agar and minimum growth inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).

Results: Silver nanoparticles from Hypericum perforatum extract showed highest absorption at 430 nm. Silver salt concentration and alkaline pH had an effect on its quantity. It is worth mentioning that resulting nanoparticle has antioxidant properties so that depending on concentration it has an inhibitory effect on free radicals with ABTS and DPPH methods; in these two methods by increasing concentration from 40 mg/ml to 250 mg/ml, amount of free radical inhibition increases and value of IC50 at  concentration of 40 mg/ml with ABTS and DPPH methods is 34.7±0.27 and 47.4±0.59, respectively The examination of results of antibacterial properties, shown that synthesized silver nanoparticles had the most antibacterial activity against standard strains of Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella enterica, respectively.

Conclusion: Hypericum perforatum extract is able to regenerate Ag+ ion and silver nanoparticles.Super nanoparticle has acceptable antioxidant and antimicrobial power.

Keywords: Hypericum perforatum, Silver Nanoparticles, Antimicrobial, Antioxidant, Green Synthesis.
Full-Text [PDF 1062 kb]   (204 Downloads)    
Semi-pilot: Experimental | Subject: Microbiology
Received: 2024/04/15 | Accepted: 2024/06/15 | Published: 2025/03/30
References
1. Swetha V, Lavanya S, Sabeena G, Pushpalaksmi E, Jenson S J, Annadurai G. Synthesis and characterization of silver nanoparticles from Ashyranthus aspera extract for antimicrobial activity studies. J Appl Sci Environ Manag 2022; 24:1161-1167. [DOI:10.4314/jasem.v24i7.6]
2. Waghmar SS, Deshmukh AM, Sadowski Z. Biosynthesis, optimization, purification and characterization of gold nanoparticles. Afr J Microbiol Res 2014;2:138-46. [DOI:10.5897/AJMR10.143]
3. Zhou Y X, Xin HI, Rahman K, Wang SJ, Peng C, Zhang H. Portulaca oleracea L. a review of phytochemistry and pharmacological effects. Biomed Res Int 2015:2015:925631. [DOI:10.1155/2015/925631]
4. Khaksar M, Vasileiadis S, Sekine R, Brunetti G, Scheckel KG, Vasilev K, et al. Chemical characterisation, antibacterial activity, and (nano)silver transformation of commercial personal care products exposed to household greywater. Environ Sci Nano 2019;6:3027-3028. [DOI:10.1039/C9EN00738E]
5. Hashemi B, Taghiloo S, Allahmoradi E, Karami M, Rahdar HA. Assessment of antibacterial effect of hydro alcoholic extract of Portulaca oleracea on the human pathogen bacteria. Journal of Sabzevar University of Medical Sciences 2018; 25: 303-8. [In Persian]
6. Lateef A, Ojo SA, Elegbede JA. The emerging roles of arthropods and their metabolites in the green synthesis of metallic nanoparticles. Nanotechnol Rev 2016; 601-622. [DOI:10.1515/ntrev-2016-0049]
7. Dousti B, Nabipour F, Hajiamraci A. Green Synthesis of Silver Nanoparticle Using Aqueous Extract of Fumaria Parviflora and Study of its Antibacterial and Antioxidant Properties. Razi Journal of Medical Sciences 2019; 26: 105-17. [In Persian]
8. Rasouli H, Popović-Djordjević J, Sayyed RZ, Zarayneh S, Jafari M, Fazeli-Nasab B. Nanoparticles: a new threat to crop plants and soil rhizobia? In: Hayat SS, Pichtel J, Faizan M, Fariduddin Q, Eds. ustainable Agriculture Reviews 41: Nanotechnology for Plant Growth and Development. Berlin: Springer International Publishing; 2020. P. 201-214. [DOI:10.1007/978-3-030-33996-8_11]
9. Kakaei K, Ghadimi G. Synthesis of silver nanoparticles based on reduced graphene oxide anelectrocatalyst for cuthod side of fuel cells. Applied Chemistry Today 2019; 14: 51-62. [In Persian]
10. Amooaghaie R, SaerI M R, Azizi M. Synthesis, characterization and biocompatibility of silver nanoparticles synthesized from _Nigella sativa_ leaf extract in comparison with chemical silver nanoparticles. cotoxicol Environ Saf 2015;120:400-8. [DOI:10.1016/j.ecoenv.2015.06.025]
11. Ghaderi RS, Kazemi M, Soleimanpour S. Nanoparticles are More Successful Competitor than Antibiotics in Treating Bacterial Infections. A Review of the Literature. Iran J Med Microbiol 2021:15:18-45. [In Persian] ‎ [DOI:10.30699/ijmm.15.1.18]
12. Ghandehari S, Homayounitabrizi M , Ardalan P. Antioxidant And Cytotoxic Properties of Green Synthesized Silver Nanoparticles from Rubia tinctorum . J Ilam Uni Med Sci 2018; 26:57-67. [In Persian] [DOI:10.29252/sjimu.26.2.57]
13. Shali R, Neamati A, Ardalan P. Investigating the Cytotoxic Effect and Antioxidant Properties of Green Synthesized Silver Nanoparticles from the Root of Persicaria Bistorta on Human Liver Cancer Cell Line (Hep G2) J Ilam Uni Med Sci 2018; 26: 133-142. [DOI:10.29252/sjimu.26.6.133]
14. Ahmad N, Sharma S. Green synthesis of silver nanoparticles using extracts of Ananas comosus. Green and Sustainable Chemistry 2012;2:141-47. [DOI:10.4236/gsc.2012.24020]
15. Anuj SA, Gajera HP, Hirpara DG, Golakiya BA. Bacterial membrane destabilization with cationic particles of nano-silver to combat efflux-mediated antibiotic resistance in gram-negative bacteria. Life Sci 2019;230:178-87. [DOI:10.1016/j.lfs.2019.05.072]
16. Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M. Biological synthesis of triangular gold nanoprisms. Nat Mater 2004;3:482-8. [DOI:10.1038/nmat1152]
17. Allafchian AR, Jalali SAH, Aghaei F, Farhang HR. Green synthesis of silver nanoparticles using Glaucium corniculatum (L.) Curtis extract and evaluation of its antibacterial activity. IET Nanobiotechnol 2018;12:574-78. [DOI:10.1049/iet-nbt.2017.0265]
18. Vélez E, Campillo G, Morales G, Hincapié C, Osorio J, Arnache O. Silver Nanoparticles Obtained by Aqueous or Ethanolic Aloevera Extracts : An Assessment of the Antibacterial Activity and Mercury Removal Capability . J Nanomater 2018:1-7. [DOI:10.1155/2018/7215210]
19. Miri A, Mahdinejad N, Ebrahimy O, Khatami M, Sarani M. Zinc oxide nanoparticles: Biosynthesis, characterization, antifungal and cytotoxic activity. Mater Sci Eng C Mater Biol Appl 2019;104:109981. [DOI:10.1016/j.msec.2019.109981]
20. Kumar Sur U, Ankamwar B, Karmakar S, Halder A, Das P. Green synthesis of Silver nanoparticles using the plant extract of Shikakai and Reetha. Materials Today: Proceedings 2018;5: 2321-29. [DOI:10.1016/j.matpr.2017.09.236]
21. Karamian R J, Kamalnejade.Green synthesis of silver nanoparticles using aqueous seed extract of Cuminum cyminum L. and evaluation of their biological activities. J Ilam Uni Med Sci 2019; 26:128-141. [In Persian] [DOI:10.29252/sjimu.26.5.128]
22. Prathap M, Alagesan A, Ranjitha Kumari BD. Anti - bacterial activities of silver nanoparticles synthesized from plant leaf extract of Abutilon indicum (L.) Sweet. J Nanostruct Chem 2014; 4:106. [DOI:10.1007/s40097-014-0106-1]
23. Kaur A, Preet S, Kumar V, Kumar R, Kumar R. Synergetic effect of vancomycin loaded silver nanoparticles for enhanced antibacterial activity. Colloids Surf B Biointerfaces 2019;176:62-69. [DOI:10.1016/j.colsurfb.2018.12.043]
24. Moshahary S, Mishra P. Synthesis of silver nanoparticles (AgNPs) using culinary banana peel extract for the detection of melamine in milk. J Food Sci Technol 2021;58:797-804. [DOI:10.1007/s13197-020-04791-x]
25. 25 Pirtarighat S, Ghannadnia M, Baghshahi S. Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J Nanostruct Chem 2019. [DOI:10.1007/s40097-018-0291-4]
26. Azizinshermeh O, Valizadeh J, Noroozifar M, Ghasemi A, Valizadeh M. Optimization, characterization and antimicrobial activity of gold nanoparticles biosynthesized using aqueous extract of Sambucus ebulus. L. Eco-phytochemical Journal of Medicinal Plants 2016;4:1-18. [In Persian]
27. Waghmar SS, Deshmukh AM, Sadowski Z. Biosynthesis, optimization, purification and characterization of gold nanoparticles. African J Microbiol Res 2014; 8: 138-46. [DOI:10.5897/AJMR10.143]
28. Dwivedi AG, Gopol K. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extracts. Coll Suf Phys Eng Asp 2010; 360:27-33. [DOI:10.1016/j.colsurfa.2010.07.020]
29. Miri A, Sarani M, Bazaz MR, Darroudi M. Plantmediated biosynthesis of silver nanoparticles using Prosopis farcta extract and its antibacterial properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015; 287-291. [DOI:10.1016/j.saa.2015.01.024]
30. Azizian Shermeh O, Valizadeh M, Valizadeh J, Taherizadeh M, Beigomi M. Phytochemical investigation and phytosynthesis of silver nanoparticles using aqueous extract of Capparis spinosa L. Modares J Biotech 2017; 8: 80-90. [In Persian]
31. Dwivedi AG, Gopol K. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extracts. Coll Suf Phys Eng Asp 2010; 360:27-33. [DOI:10.1016/j.colsurfa.2010.07.020]
32. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005; 16:23-46. [DOI:10.1088/0957-4484/16/10/059]
33. Brian MO, hemachitra P, Deepa R, Selvi VS. Synthesis of silver nanoparticles and its antibacterial activity from Moringa Oleifera, murraya Koingii and Ocimum sanctum against E.Coli and S.aureus. Der Pharmacia Lettre 2016; 8: 150-60.
34. Arokiyaraj S, Vincent S, Saravanan M, Green. Synthesis of silver nanoparticles using Rheum Palmatum root extract and their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Artif Cells Nanomed Biotechnol 2016; 45: 372-379. [DOI:10.3109/21691401.2016.1160403]
35. Rodinol S, Butu A, Petrache P, Butu M, Dinupirvu C, Cornea CA. Evaluation of the antimicrobial and antioxidant activity of Sambucus ebulus extract. Farmacia 2015; 5:751-4.
36. Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gramnegative bacteria. Nanomedicine 2012; 8: 37-45. [DOI:10.1016/j.nano.2011.05.007]
37. Dallas P, Sharma VK, Zboril R. Silver polymeric nanocomposites as advanced antimicrobial agents: classification, synthetic paths, applications, and perspectives. Adv Colloid Interface Sci 2011;166:119-35. [DOI:10.1016/j.cis.2011.05.008]
38. Abdelaziz MS, Elnekeety AA, Abdelwahhab MA. Antioxidant and antibacterial activity of Silver nanoparticles biosynthesizes using chenopodium murale leaf extract. J Saudi Chem Soc 2014;18:356-63. [DOI:10.1016/j.jscs.2013.09.011]
39. Sudha A, Jeyakanthan J, Srinivasan P. Green synthesis of silver nanoparticles using Lippia nodiflora aerial and evaluation of their antioxidant, Resource-Efficient Technologies 2017;3: 506-515. [DOI:10.1016/j.reffit.2017.07.002]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Najafi F, Shafiei Z. Investigating the antioxidant properties and antimicrobial activity of green synthesis of silver nanoparticle by using the aqueous extract of Hypericum perforatum. MEDICAL SCIENCES 2025; 35 (1) :24-34
URL: http://tmuj.iautmu.ac.ir/article-1-2100-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 35, Issue 1 (spring 2025) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.06 seconds with 36 queries by YEKTAWEB 4710