[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 34, Issue 2 (summer 2024) ::
MEDICAL SCIENCES 2024, 34(2): 163-169 Back to browse issues page
New Delhi metallo-beta-lactamase-producing Enterobacteriaceae isolated from urine sample in Karaj
Sogol Neysari Tabrizi1 , Azam Haddadi 2, Ebrahim Babapour3
1- MSc, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
2- Assistant professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , haddadi.azam@gmail.com
3- Assistant professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran
Abstract:   (417 Views)
Background: Urinary tract infection is one of the most important infectious diseases. Beta-lactam antibiotics, especially the third and fourth generations of cephalosporins, are effective in treating this infection. The production of beta-lactamase enzyme by bacteria is the most important mechanism of resistance to various beta-lactam antibiotics, which poses a serious threat to the use of these drugs in the future. The aim of this study is to investigate the presence of new beta-lactamases in Enterobacteriaceae isolated from urinary tract infections.
Materials and methods: In this cross-sectional descriptive research, 100 isolates of Enterobacteriaceae were isolated from urine samples collected from the hospitals and laboratories of Alborz province. The isolates were identified by standard biochemical and microbial tests. The pattern of antibiotic resistance of the isolates to 11 different antibiotics was investigated by the Kirby Bauer method. The isolates were analyzed phenotypically in terms of β-lactamase production (NDM-1). In the case of isolates with β-lactamase blaNDM-1, PCR was done with the help of specific primers, and finally the PCR product was sequenced and analyzed.
Results: The pattern of antibiotic resistance was observed as follows: ampicillin (85%), imipenem (70%), kanamycin (66%), cefotaxime (51%), ceftriaxone (51%), tetracycline (50%), ceftazidime (% 49), ciprofloxacin (46%), neurofloxacin (36%), gentamicin (29%), meropenem (27%). The results of PCR showed that 2% of the isolates had blaNDM-1 gene.
Conclusion: The increase of antibiotic resistance in clinical isolates and the emergence of new beta-lactamases warn that the policy of using antibiotics to treat bacterial infections should be changed.
 
Keywords: Urinary tract infection, Antibiotic resistance, NMD-1, PCR.
Full-Text [PDF 434 kb]   (189 Downloads)    
Semi-pilot: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2023/09/20 | Accepted: 2023/11/12 | Published: 2024/06/30
References
1. Ghafourian S, Sadeghifard N, Soheili S, Sekawi Z. Extended Spectrum Beta-lactamases: Definition, Classification and Epidemiology. Curr Issues Mol Biol 2015;17:11-21.
2. Janredini A, Heydari F, Tagvi F, Shekouh M. Investigation of frequency and antibiotic resistance pattern of Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infection in outpatients referred to Amiralmomenin Ali hospital in Gerash city. JRUMS 2018; 17;75-84. [In Persian]
3. Haddadi A, Yekefallah F. Frequency determination of blaTEM & blaSHV in the ExtendedSpectrum Beta-Lactamase producing Escherichia coli isolates from Karaj. Iran J Med Microbiol 2017; 11:75-80. [In Persian]
4. Wilson ME, Chen LH. NDM-1 and the Role of Travel in Its Dissemination. Curr Infect Dis Rep. 2012;14:213-26. [DOI:10.1007/s11908-012-0252-x]
5. Lowman W, Sriruttan C, Nana T, Bosman N, Duse A, Venturas J, Clay C, Coetzee J. NDM-1 has arrived: first report of a carbapenem resistance mechanism in South Africa. S Afr Med J 2011;101:873-5.
6. Paterson DL. Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Med. 2006;119:S20-8. [DOI:10.1016/j.amjmed.2006.03.013]
7. Weinstein MP, Lewis JS 2nd. The Clinical and Laboratory Standards Institute Subcommittee on Antimicrobial Susceptibility Testing: Background, Organization, Functions, and Processes. J Clin Microbiol 2020;58:e01864-19. [DOI:10.1128/JCM.01864-19]
8. Olivier A, Herbert B, Sava B, John D, John G, Tine H, et al. Scientific opinion on the public health risks of bacterial strains producing extended‐spectrum β‐lactamases and/or AmpC β‐lactamases in food and food‐producing animals. EFSA J 2011; 9:2322. [DOI:10.2903/j.efsa.2011.2322]
9. Shahbazi B, Narenji H. Comparison of four DNA extraction methods from Gram-negative and Gram-positive bacteria. Zanko J Med Sci 2014; 15:9-16.
10. Askari Sh, Haddadi A, Harzandi N. Frequency of TEM and SHV in the extended-spectrum beta-lactamase producing Klebsiella isolates from Karaj city. J Med Sci IAU 2015; 25:277-282.
11. Dehghani M, Haddadi A, Shavandi M. Multiplex PCR Study of Plasmid-Mediated AmpC Beta-Lactamase Genes in Clinical Isolates of Escherichia coli. J Med Bacteriol 2016; 5: 21-28.
12. Kohsari E, Zahedi B, Abbasian S, Fakhriasri H, SamadiKafil H, Mohammadzadeh R, et al. A review of common laboratory methods for detection of carbapenemases in gram-negative bacilli. RJMS 2016; 24:165;47-65.
13. Shivaee A, Shahbazi SH, Soltani A, Ahadi E. Evaluation of the prevalence of broad -spectrum beta -lactamases (ESBLs) and carbapenemase genes in Klebsiella pneumoniae strains isolated from burn wounds in patients referred to Shahid Motahari Hospital in Tehran. Med Sci J IAU 2019; 29:232-239. [DOI:10.29252/iau.29.3.232]
14. Afrugh P, Mardaneh J, Kaidani A, Serajian A, Abbasi P, Yahivi M. Distribution and antimicrobial susceptibility pattern of Gram negative bacteria causing urinary tract infection (UTI) and detection New Delhi Metallo -beta - lactamase - 1 (NDM -1) producing isolates in Ahwaz. ISMJ 2016; 19:15-26.
15. Mohammadi M, Bahrami N, Faghri J. The evaluation of antibiotic resistance pattern and frequency of blaVIM and blaNDM genes in isolated Acinetobacter baumannii from hospitalized patients in Isfahan and Shahrekord. RJMS 2020; 27: 143-156.
16. Aruhomukama D, Najjuka CF, Kajumbula H, Okee M, Mboowa G, Sserwadda I, et al. blaVIM-and blaOXA-mediated carbapenem resistance among Acinetobacter baumannii and Pseudomonas aeruginosa isolates from the Mulago hospital intensive care unit in Kampala, Uganda. BMC Infect Dis 2019; 19:1-8. [DOI:10.1186/s12879-019-4510-5]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Neysari Tabrizi S, Haddadi A, Babapour E. New Delhi metallo-beta-lactamase-producing Enterobacteriaceae isolated from urine sample in Karaj. MEDICAL SCIENCES 2024; 34 (2) :163-169
URL: http://tmuj.iautmu.ac.ir/article-1-2152-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 34, Issue 2 (summer 2024) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.06 seconds with 36 queries by YEKTAWEB 4660