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:: Volume 32, Issue 2 (summer 2022) ::
MEDICAL SCIENCES 2022, 32(2): 132-138 Back to browse issues page
Morphometric study of rat jejunum enterocyte after L-Arginine and L-NAME prescription
Shabnam Movassaghi1, Zahra Nadia Sharifi2, Seyed Mohammad Hossein Noori Mugahi 3
1- Department of Anatomical Sciences & cognitive neuroscience, Faculty of medicine, Tehran medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Anatomical Sciences, Faculty of Medicine, Tehran medical Sciences, Islamic Azad University,Tehran,Iran
3- Department of Anatomical Sciences & cognitive neuroscience, Faculty of medicine, Tehran medical Sciences, Islamic Azad University, Tehran, Iran - Department of Anatomical Sciences, Faculty of Medicine, Tehran medical Sciences, Tehran University of Medical Sciences, Tehran, Iran , noorimoo@gmail.com
Abstract:   (614 Views)
Background: Nitric Oxide (NO) is a very important signaling molecule which acts as a regulator of many physiological processes in many tissues including epithelial cell of gastrointestinal tract. In this study we investigated the effects of L-Arginine as a NO progenitor and L-NAME as a NO inhibitor on epithelial cell number and height of Jejunum in female rats.
Materials and methods: 40 female rats were divided into 5 groups, containing 8 rats in each group. Except the control group, the other groups received normal saline (2 ml/kg), L-Arginine (200mg/kg), L-NAME (20mg/kg) and a mixture of two substances for L-Arginine & L-NAME group intraperitoneally for 3 days. 2 weeks later after anesthesia with ether, jejunum was expelled out and after tissue processing and staining with H&E method, the changes were assessed via light microscopy. Cell number and height were evaluated using Image Tools3 Microsoft. Statistical analysis was made by One-Way ANOVA followed by Tukey post hoc test to evaluate the statistical significance between different groups. A value of p< 0.05 was considered statistically significant.
Results: There was a significant increase in the cell number and height of jejunal epithelium in L-Arginine group (P<0.05). Whereas no significant difference was observed between L-NAME, L-Arginine +L-NAME and control, Normal Saline groups.
Conclusion: L- Arginine can result in proliferation of Jejunum epithelial cells whereas L-NAME has no effect on these cells.
 
Keywords: Nitric oxide, L-Arginine, L-NAME, Epithelial cell, Jejunum
Full-Text [PDF 514 kb]   (174 Downloads)    
Semi-pilot: Experimental | Subject: Histology
Received: 2021/06/30 | Accepted: 2022/05/31 | Published: 2022/07/1
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Movassaghi S, Sharifi Z N, Noori Mugahi S M H. Morphometric study of rat jejunum enterocyte after L-Arginine and L-NAME prescription. MEDICAL SCIENCES 2022; 32 (2) :132-138
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Volume 32, Issue 2 (summer 2022) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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