[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 32, Issue 2 (summer 2022) ::
MEDICAL SCIENCES 2022, 32(2): 132-138 Back to browse issues page
Morphometric study of rat jejunum enterocyte after L-Arginine and L-NAME prescription
Shabnam Movassaghi1, Zahra Nadia Sharifi2, Seyed Mohammad Hossein Noori Mugahi 3
1- Department of Anatomical Sciences & cognitive neuroscience, Faculty of medicine, Tehran medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Anatomical Sciences, Faculty of Medicine, Tehran medical Sciences, Islamic Azad University,Tehran,Iran
3- Department of Anatomical Sciences & cognitive neuroscience, Faculty of medicine, Tehran medical Sciences, Islamic Azad University, Tehran, Iran - Department of Anatomical Sciences, Faculty of Medicine, Tehran medical Sciences, Tehran University of Medical Sciences, Tehran, Iran , noorimoo@gmail.com
Abstract:   (614 Views)
Background: Nitric Oxide (NO) is a very important signaling molecule which acts as a regulator of many physiological processes in many tissues including epithelial cell of gastrointestinal tract. In this study we investigated the effects of L-Arginine as a NO progenitor and L-NAME as a NO inhibitor on epithelial cell number and height of Jejunum in female rats.
Materials and methods: 40 female rats were divided into 5 groups, containing 8 rats in each group. Except the control group, the other groups received normal saline (2 ml/kg), L-Arginine (200mg/kg), L-NAME (20mg/kg) and a mixture of two substances for L-Arginine & L-NAME group intraperitoneally for 3 days. 2 weeks later after anesthesia with ether, jejunum was expelled out and after tissue processing and staining with H&E method, the changes were assessed via light microscopy. Cell number and height were evaluated using Image Tools3 Microsoft. Statistical analysis was made by One-Way ANOVA followed by Tukey post hoc test to evaluate the statistical significance between different groups. A value of p< 0.05 was considered statistically significant.
Results: There was a significant increase in the cell number and height of jejunal epithelium in L-Arginine group (P<0.05). Whereas no significant difference was observed between L-NAME, L-Arginine +L-NAME and control, Normal Saline groups.
Conclusion: L- Arginine can result in proliferation of Jejunum epithelial cells whereas L-NAME has no effect on these cells.
Keywords: Nitric oxide, L-Arginine, L-NAME, Epithelial cell, Jejunum
Full-Text [PDF 514 kb]   (174 Downloads)    
Semi-pilot: Experimental | Subject: Histology
Received: 2021/06/30 | Accepted: 2022/05/31 | Published: 2022/07/1
1. Grasa L, Rebollar E, Arruebo M.P, Plaza M.A, Murillo M.D. Grasa L. The role of NO in the contractility of rabbit small intestine in vitro: effect of K+ channels. J Physiol Pharmacol 2005;56:407-19.
2. Tejero J, Shiva S, Gladwin MT. Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation. Physiol Rev 2019;99:311-379. [DOI:10.1152/physrev.00036.2017]
3. Weckman AM, McDonald CR, Baxter JB, Fawzi WW, Conroy AL, Kain KC. Perspective: L-arginine and L-citrulline Supplementation in Pregnancy: A Potential Strategy to Improve Birth Outcomes in Low-Resource Settings. Adv Nutr 2019;10:765-777. [DOI:10.1093/advances/nmz015]
4. Bignon E, Rizza S, Filomeni G, Papaleo E. Use of Computational Biochemistry for Elucidating Molecular Mechanisms of Nitric Oxide Synthase. Comput Struct Biotechnol J 2019;17:415-429. [DOI:10.1016/j.csbj.2019.03.011]
5. Eroglu E, Hallström S, Bischof H, Opelt M, Schmidt K, Mayer B, et al. Real-time visualization of distinct nitric oxide generation of nitric oxide synthase isoforms in single cells. Nitric Oxide 2017;70:59-67. [DOI:10.1016/j.niox.2017.09.001]
6. Villanueva C, Giulivi C. Subcellular and cellular locations of nitric oxide synthase isoforms as determinants of health and disease. Free Radic Biol Med 2010;49:307-316. [DOI:10.1016/j.freeradbiomed.2010.04.004]
7. Liang YC, Liu HJ, Chen SH, Chen CC, Chou LS, Tsai LH. Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice: Roles of nitric oxide and prostaglandin E2. World J Gastroenterol 2005; 11: 357-61. [DOI:10.3748/wjg.v11.i3.357]
8. Sanchez LC. Disorders of the Gastrointestinal System. Equine Internal Med 2018;709-842. [DOI:10.1016/B978-0-323-44329-6.00012-7]
9. Noori Mugahi SMH, Azar Nia M, Ghobeh Mohager N. Effects of nitric oxide on qualitative and quantitative differentiation of mouse uterus. Iranian Anat Scenic J 2004; 2: 61-7. [In Persian]
10. Hogg N, Struck A. Inhibition of macrophage-dependent low density lipoprotein oxidation by nitric oxide donors. J Lipid Res 1999; 36:1756-62. [DOI:10.1016/S0022-2275(20)41494-4]
11. Noori SMH, Mahmmoudzadeh Sagheb HR, Heidari Z, eds. Applied methods and terminology of histotechnique, stereology & morophology. 3rd ed. Tehran: Tehan Uni Med Sci Public; 2009. P.71, 95-8. [In Persian]
12. 12-Mu K, Yu Sh, Kitts D.D. The Role of Nitric Oxide in Regulating Intestinal Redox Status and Intestinal Epithelial Cell Functionality. Int J Mol Sci 2019; 20: 1755. [DOI:10.3390/ijms20071755]
13. Magierowski M, Magierowska K, Kwiecien S, Brzozowski T.Gaseous. Mediators Nitric Oxide and Hydrogen Sulfide in the Mechanism of Gastrointestinal Integrity, Protection and Ulcer Healing. Molecules 2015; 20: 9099-123. [DOI:10.3390/molecules20059099]
14. Kochar NI, Chandewal AV, Bakal RL, Kochar PN. Nitric Oxide and the Gastrointestinal Tract. Int J Pharmacol 2011; 7:31-9. [DOI:10.3923/ijp.2011.31.39]
15. Puiman PJ, Stoll B, van Goudoever JB, Burrin DG. Enteral Arginine Does Not Increase Superior Mesenteric Arterial Blood Flow but Induces Mucosal Growth in Neonatal Pigs. J Nutr 2010; 24:1-8. [DOI:10.3945/jn.110.131888]
16. Roy HK, Wali RK, Kim Y, Liu Y, Hart J, Kunte DP, et al. Inducible nitric oxide synthase (iNOS) mediates the early increase of blood supply (EIBS) in colon carcinogenesis. FEBS Lett 2007;581:3857-62. [DOI:10.1016/j.febslet.2007.07.012]
17. Hays E, Bonavida B. Nitric Oxide-Mediated Enhancement and Reversal of Resistance of Anticancer Therapies. Antioxidants (Basel) 2019;8:407. [DOI:10.3390/antiox8090407]
18. Akgün-dar K, Balci H, Yagci A, Kapucu A, Uyaner I. Effects of leptin on the epithelial cell proliferation from the small intestine and nitric oxide (NO) production in rats. Revue Méd Vét 2007; 158: 161-8.
19. Driák D, Osterreicher J, Vávrová J, Řeháková Z, Vilasová Z. Morphological changes of rat jejunum after whole body gamma-irradiation and their impact in biodosimetry. Physiol Res 2008;57:475-479. [DOI:10.33549/physiolres.931027]
20. Nasr I, Nasr I, Beyers C, Chang F, Donnelly S, Ciclitira PJ. Recognising and Managing Refractory Coeliac Disease: A Tertiary Centre Experience. Nutrients 2015;7:9896-907. [DOI:10.3390/nu7125506]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Movassaghi S, Sharifi Z N, Noori Mugahi S M H. Morphometric study of rat jejunum enterocyte after L-Arginine and L-NAME prescription. MEDICAL SCIENCES 2022; 32 (2) :132-138
URL: http://tmuj.iautmu.ac.ir/article-1-1880-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 32, Issue 2 (summer 2022) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4570