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Production of collagen type I and its using in medical practice
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Mohammad Karim Rahimi   1, Ramin Khajavi2 , Mohammad Amin Mahdavi Hezaveh3 , Elnaz Ebrahimi Hour4 |
1- Department of Microbiology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran , mohammadkrahimi@yahoo.com
2- Postgraduate center, Tehran South Branch, Islamic Azad University, Tehran, Iran
3- Tehran south Branch, Islamic Azad University, Tehran, Iran
4- Tehran Medical Branch, Islamic Azad University, Tehran, Iran |
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Abstract: (33530 Views) |
Background: Collagen is a protein with specific importance in pharmacy and medicine. The aim of this study was extraction of collagen type I by optimum using of Iranian fishes of Persian Gulf and also debris of Shilat industry.
Materials and methods: Extraction of collagen type I from the proper specimens was performed by two methods of acidic and alkaline. In acidic method, first, the lipids separated, then, by using HCL 5%, calcium elements were extracted. The pH of medium neutralized and hydrolysis was performed in high stream water vapor. The material have been filtered and in the vicinity of heat in vacuum state collagen concentrated. Then the sheets of collagen, grinding was done. In the alkaline method, the specimens were treated by sodium hydroxid 4% for 3 weeks at room temperature.
Results: The rate of separated collagen from fish hasn’t significant difference between alkaline and acidic methods. For example, mean rate of 20.81% for collagen extraction from skin of fishes in alkaline method and mean of 19.76% in acidic method was detected. The collagen specimens extracted by these methods have equal or higher quality in comparison with specimens extracted from other sources.
Conclusion: According to costless of primary materials, such as debris of fishes, in the production of collagen in Iran and import of collagen is expensive, domestic production is saving money. |
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| Keywords: Skin and debris of fish, Extraction method, Collagen type I, Medical practices |
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Full-Text [PDF 560 kb]
(9041 Downloads)
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Semi-pilot: Basic |
Subject:
Labratory Sciences
Received: 2011/02/12 | Accepted: 2013/05/28 | Published: 2013/05/28
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