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Showing 2 results for Keivani

Sogol Keivani, Firouzeh Salamat, Masoud Emami, Parvaneh Adimi, Golamreza Amin,
Volume 16, Issue 3 (Fall 2006)
Abstract

Backgrouns: The purpose of this study was to evaluate the effects of Pistacia vera's pericarp extract on some common dermatophytic and saprophytic fungi of Iran and identifying its probable role to be used instead of chemical drugs.
Materials and Methods: After collecting pericarp of Pistacia vera, drying and making it powder, extracts were obtained by using Percolation method with methanol and n-Hexan. To evaluate the anti-fungal activity of the extract, different dilutions of the extract (30-600 mg/ml) were prepared and tested against each fungus and Minimum Inhibition Concentration (MIC) was measured via disc diffusion & broth dilution methods. The tested fungi were three types of dermatophytes (Trichophyton mentagrophytes, Microsporum canis, Epidermophyton floccosum) and two types of saprophytes (Aspergillus niger and Candida albicans).
Results: The results showed that n-Hexan extract in disc diffusion method has no significant effect on the fungi, but it could inhibit Epidermophyton floccosum growth in 337mg/ml dilution and Microsporum canis growth in 450mg/ml dilution. For methanolic extract in broth dilution method, 60mg/ml was inhibitor for Epidermophyton floccosum and Trichophyton mentagrophytes and 240mg/ml dilution for Microsporum canis growth. In disc diffusion method we had 17 millimeter inhibitory zone around the pure extract in Trichophyton mentagrophytes. This extract had no anti-fungal effect against Aspergillus niger, but inhibited the growth of Candida albicans in 120mg/ml dilution and also calculated MBC for Candida albicans was 600mg/ml.
Conclusion: Our research showed Pistacia vera's pericarp extract has different anti-fungal effects on experimented fungi.
Firuzeh Salamat, Sogol Keivani, Masoud Emami, Gholamreza Amin, ,
Volume 16, Issue 4 (Winter 2006)
Abstract

Background: Antifungal property of Juglans regia has not been proved yet, therefore, the present study we designed to examine the effect of Juglans regia pericarp on the growth of different fungi, particularly dermatophytes.
Materials and methods: The extract has been prepared by methanol solution using percolation method according to NCCLS protocol. Then, the fractions have been examined on fungi inoculums. For MBC estimation, it has been cultured on PDA medium.
Results: Juglans regia extract completely prevented growth of Epidermophyton floccosum and Trichophyton mentagrophytes in fraction of 120 mg/ml, Microsporum canis in fraction of 450 mg/ml, and Candida albicans in fraction of 337 mg/ml, meanwhile, it reduced colony growth of Aspergillus niger compared with the control.
 Conclusion: We have demonstrated that the growth of three dermatophyes and Candida albicans was completely inhibited by Juglans regia pericarp extract.

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فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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