Fatemeh Dahehpour, Abdolreza Sabokrouh, Mina Samsam Shariat,
Volume 34, Issue 3 (10-2024)
Abstract
Background: Neuro degenerative diseases, such as schizophrenia and Parkinson, are the main disorders in the human brain. The changes in the expression of dopa decarboxylase (DDC) and dopamine beta hydroxylase (DBH) genes are main causes, which play an important role in the level of dopamine in the brain. In this study, the correlation between the expression levels of DDC and DBH and these diseases has been investigated.
Materials and methods: In this research, the target people were divided into three groups including group A (healthy people), group B (people with schizophrenia) and group C (people with Parkinson). Each group includes 15 individuals. After RNA extraction and cDNA synthesis, the expression level of DDC and DBH genes expression was determined through Real-time PCR.
Results: The DDC expression showed decreased value in group C 0.08(0.03, 0.25) compared to group A 12.81(3.92, 31.34), and the DBH had increased value in group C 3.18(1.39, 8.14) compared to group A 0.22(0.11, 0.42). On the contrary, the DDC expression in group B revealed increased value 7.06(3.34, 9.75) compared to group A 0.14(0.1,0.3) and the DBH expression in group B had decreased value 0.04(0.03,0.58) compared to group A 31.34(16.51,82.27).
Conclusion: Our results showed that DDC and DBH genes expression plays a role in the occurrence of the diseases. There was no significant relationship between the genes, so by monitoring the expression level of these two genes and finding drugs to regulate the expression of them, we can find a way to prevent and treat the diseases.
