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Background: Staph aureus continues to be a major cause of community-acquired and health-care related infection worldwide. The emergence of high levels penicillin resistance Staph followed by the development and spread of strains resistant to the semi synthetic penicillin’s has made the therapy of staphylococcal disease a global challenge. At present, vancomycin- intermediate and resistant Staph aureus has been spreading in some hospitals. The purpose of the present study was to determine the distribution of S. aureus at various sites of eye surgery room in pre and post operation and its antibiotic sensitivity at Golpayegani hospital.
Materials and methods: Common antibiotic against S. aureus used for antibiotic testing. The determination of MIC was performed with the agar dilution method according to the procedure outlined by NCCLS. This study was descriptive and was performed in Golpayegani hospital of Qom during the first semester of 2005. The statistical analysis was done by using T and paired T- Test.
Results: The frequency of Staph aureus was lower in preoperative setting during the Spring and Summer seasons. In preoperative sampling, most of the Staph aureus were isolated from broom and floor, while in postoperative sampling, most of the isolates were from broom, floor and anesthetic mask. All of the isolates revealed a totally different antimicrobial susceptibility pattern so that all of the isolates were susceptible to Vancomycin and Methicilin, however, postoperative isolates showed resistance to Carboxycilin, Tetracyclin, Cephazolin and Penicillin. Conclusion: According to the higher frequency of antimicrobial resistant isolates during warm months, probably insufficient disinfectant technique and temperature may play a major role in bacterial resistance. Thus, for effective removal of all bacteria, immediate application of antiseptic agents after surgery is recommended.