AU - HASHEMI, Mehrdad AU - GHarashi, Seyed Ali AU - Parivar, Kazaem AU - Dizaji, Abdolkhalegh AU - Shamsara, Mehdi AU - Doustar, Yousof AU - Bakhshayesh, Masoumeh TI - Study of cloned gene-related protein Vp2 of infectious bursal disease virus- induced apoptosis in human lymphoma B cells in vitro PT - JOURNAL ARTICLE TA - iau-tmuj JN - iau-tmuj VO - 16 VI - 4 IP - 4 4099 - http://tmuj.iautmu.ac.ir/article-1-308-en.html 4100 - http://tmuj.iautmu.ac.ir/article-1-308-en.pdf SO - iau-tmuj 4 ABĀ  - Background: Apoptosis or programmed cell death (PCD) is an important mechanism in both development and homeostasis in adult tissue for the removal of superfluous cells, while its induction is an effective therapeutic approach for cancer. The aim of the present study was to evaluate the effect of cloned gene-related protein Vp2 of infectious bursal disease virus in human lymphoma B cells. Material and methods: In present study after cloning the Vp2 gene in Pichia pastoris system, the Vp2 protein was expressed. Cellular vital capacity was determined by MTT. Then effect of Vp2 protein in human lymphoma B cells was examined by Hoechst staining and flowcytometry techniques, respectively. Results: During MTT, human lymphoma B cell lines revealed to have a meaningful apoptosis at 1mg and 5mg protein concentrations when compared with controls (p CP - IRAN IN - Department of Molecular Genetics, Islamic Azad University, Tehran Medical Branch LG - eng PB - iau-tmuj PG - 191 PT - YR - 2006