fa ارتباط پلی مورفیسم rs2274907 ژن امنتین با بیماری دیابت نوع 2 در جمعیت ایرانی بيوتكنولوژي biotechnology تجربي Experimental <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">دیابت یکی از رایج&shy;ترین بیماری&shy;های مزمن متابولیک جهان است که به دنبال کاهش ترشح انسولین و یا مقاومت به انسولین</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">(IR)</span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal"> و تجمع چربی در بافت چربی احشایی ایجاد می&shy;شود. امنتین پروتئین مترشحه از بافت چربی است که با میزان ابتلا به دیابت در ارتباط است. هدف از انجام این تحقیق بررسی ارتباط پلی مورفیسم </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">rs2274907 </span></span></i><i>&nbsp;</i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">ژن امنتین-۱ با بیماری دیابت بود</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="font-weight:normal">. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.3pt"><span style="font-weight:normal">این </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">مطالعه موردی- شاهدی بر روی ۷۰ بیمار مبتلا به دیابت و ۷۰ فرد سالم به عنوان&nbsp; شاهد انجام شد. میزان قند خون ناشتا، تری گلیسرید، کلسترول، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">HDL</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">LDL</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در افراد با دستگاه اتوآنالایز و سطح امنتین و انسولین با روش الایزا اندازه گیری شد. تعیین ژنوتیپ با استفاده از روش </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">PCR-RFLP</span></span></span></i><i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> انجام گرفت.</span></span></span></span></i><i>&nbsp; </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&shy;</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">سن، نمایه توده بدنی</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">LDL</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">،</span></span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">کلسترول، تری گلیسرید، قند خون ناشتا و مقاومت به انسولین در افراد بیمار و سالم تفاوت معنی&shy;داری داشت (05/ 0></span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). تفاوت معنی&shy;داری در سطح امنتین در افراد دیابتی و کنترل مشاهده نشد (05/0<</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). سطح امنتین در ژنوتیپ&shy;های مختلف&nbsp; </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">rs2274907</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">&nbsp; در افراد سالم متفاوت بود (05/ 0></span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). تحلیل رگرسیونی ژنوتیپ&shy;ها نشان داد که ژنوتیپ پلی مورفیسم </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">rs2274907</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">با دیابت ارتباطی نداشت (05/ 0< </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">P</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br> <b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;<b>گیری</b><i>:</i></span></span> <i><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt">مطالعه حاضر نشان داد ارتباط معنی&shy;داری بین ژنوتیپ </span></span></span><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.2pt">rs2274907</span></span></span></b><span dir="RTL" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="letter-spacing:-.2pt"> و دیابت نوع دو وجود ندارد</span></span></span></i> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Diabetes is one of the most common chronic metabolic diseases in the world that is caused by decreased insulin secretion or insulin resistance and fat accumulation in visceral adipose tissue (IR). Omentin is a protein inferred from adipose tissue that is associated with the rate of diabetes. The aim of this study was to investigate the relationship between rs2274907 polymorphism of amentine-1 gene with diabetes. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: This case-control study was conducted on 70 patients with diabetes and 70 healthy individuals as control. FBS, TG, CHOL, HDL, and LDL were measured in the subjects and genotype determination was performed using PCR-RFLP. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: Age, body mass index, LDL, cholesterol, triglyceride, fasting high sugar and insulin resistance were significantly different between patients and healthy individuals (p<0.05). No significant difference was observed in omentin level in diabetic compared to control patients (p>0.05). Omentin levels in different genotypes rs2274907 were different in healthy subjects, but the difference was not significant. Omentin levels in different genotypes rs2274907 were different in healthy subjects (p<0.05). Genotype regression analysis showed that genotypes in rs2274907 polymorphism were not related to diabetes (p>0.05).</span></span></span></span></span><br> <span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: This study revealed that there was no significant relationship between rs2274907 genotype and type 2 diabetes.</span></span></span></span></span></span><br> &nbsp; دیابت نوع ۲, ادیپوکین, ژن امنتین, مقاومت به انسولین Type 2 diabetes, Adipokine, Omentin gene, Insulin resistance 44 52 http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-944-6&slc_lang=fa&sid=1 Arezo Nazari آرزو نظری 100319475328460010228 100319475328460010228 No MSC, Department of Biotechnology, Faculty of Biotechnology, Medicine Branch, Azad University, Tehran, Iran کارشناس ارشد، گروه بیوتکنولوژی ، علوم پزشکی تهران دانشگاه آزاد اسلامی تهران، ایران Mahnaz Mohammadi مهناز محمدی mh_mohamadi@yahoo,com 100319475328460010229 100319475328460010229 Yes Assistant Professor, Department of Biology, Faculty of Biological Sciences Islamshahr Branch, Azad University, Islamshahr, Tehran, Iran استادیار، گروه زیست شناسی، واحد اسلامشهر دانشگاه آزاد اسلامی ، اسلامشهر، ایران Masoumeh Nezhadali Lamfjani معصومه نژاد علی لمفجانی 100319475328460010230 100319475328460010230 No Assistant Professor, Department of Biology, Faculty of Biological Sciences Islamshahr Branch, Azad University, Islamshahr, Tehran, Iran استادیار، گروه زیست شناسی، واحد اسلامشهر دانشگاه آزاد اسلامی ، اسلامشهر، ایران Tahereh Naji طاهره ناجی 100319475328460010231 100319475328460010231 No Associate Professor, Faculty of Biological Sciences, Department of Biology Medicine Branch, Azad University, Tehran, Iran دانشیار، گروه زیست شناسی، علوم پزشکی تهران، دانشگاه آزاد اسلامی تهران، ایران