Medical Sciences Journal of Islamic Azad University
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی تهران
MEDICAL SCIENCES
Medical Sciences
http://tmuj.iautmu.ac.ir
1
admin
1023-5922
2008-3386
000
10.61882/iau
000
1023-5922
000
fa
jalali
1402
2
1
gregorian
2023
5
1
33
2
online
1
fulltext
fa
اثر نارینجین بر آسیب سلولی هیپوکامپ و سیستم دفاع آنتی اکسیدانی مغز قدامی جنین در مدل حیوانی نارسایی رحمی جفتی
The effect of Naringin on hippocampal cell damage and the antioxidant defense system of the fetal forebrain in an animal model of uteroplacental insufficiency
زيست شناسي جانوري
Animal Biology
تجربي
Experimental
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">سابقه و هدف</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:Nazanin"><span style="font-weight:normal">:</span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">نارسایی رحمی-جفتی (</span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">UPI</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">) </span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">با القاء استرس اکسایشی در مغز جنین منجر به اختلال در تکامل آن میگردد. مطالعه حاضر به ارزیابی اثرات نارینجین بر سیستم دفاع آنتی اکسیدانی در مغز قدامی جنین و پیشگیری از آسیب سلولی هیپوکامپ به دنبال </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal">UPI</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal"> در موش صحرایی پرداخته است. </span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-weight:normal"></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">روش بررسی</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt"><span style="font-weight:normal">:</span></span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.3pt">:</span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">تعداد 20 سر موش صحرایی باردار نژاد ویستار به صورت تصادفی در 4 گروه کنترل، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI+Saline</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> (نارسایی رحمی-جفتی+نرمالسالین)، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> UPI+Nar50</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> (نارسایی رحمی-جفتی+نارینجین با دوز </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">mg/kg </span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> 50) و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI+Nar100</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> (نارسایی رحمی-جفتی+نارینجین با دوز </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> mg/kg</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">100) تقسیم شدند. جهت القاء </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، انسداد دائم عروق قدامی رحم در روز 18 بارداری صورت گرفت. تجویز دهانی نارینجین و نرمال سالین از روز 12 تا 18 بارداری انجام شد. در روز 21 بارداری، سنجش سطح کاتالاز (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)، سوپراکسید دیسموتاز (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">SOD</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) و ظرفیت آنتیاکسیدانیتام (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) به روش الایزا و مالوندیآلدهید (</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">) به روش تیوباربیتوریک اسید در مغز قدامی جنین و ارزیابی تراکم نورونی در نواحی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA3</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> هیپوکامپ صورت گرفت.</span></span></span></span></i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.3pt"><span style="font-weight:normal"></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt">یافته</span></span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">­</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt">ها:</span></span></span> <i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">کاهش معنی­دار در فعالیت </span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">مغزی </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">بافتی آنزیم </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">SOD</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و تراکم نورونی در نواحی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1/CA3</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> هیپوکامپ همراه با افزایش </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">معنی­دار</span></span></span></span></i><i> </i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">در گروه </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI+Salin</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> نسبت به گروه کنترل دیده شد (05/0</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p<</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">). در حالیکه گروههای دریافت کننده نارینجین</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">،</span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> افزایش معنی­دار</span></span></span></span></i><i> </i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CAT</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">SOD</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">، </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">TAC</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> و تراکم نورونی </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">CA1/CA3</span></span></span></i><i> </i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">را با کاهش </span></span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">معنی­دار</span></span></span></span></i><i> </i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">MDA</span></span></span></i><i> </i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">نسبت به گروه </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI+Saline</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> دیده شد (05/0</span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">p<</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">)</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتیجه</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">گیری</span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="font-weight:normal">:</span></span></span></i> <i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">تجویز نارینجین پیش از القاء </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> با تقویت سیستم آنتی اکسیدانی در مغز قدامی جنین سبب پیشگیری از عوارض عصبی ناشی از </span></span></span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="letter-spacing:-.2pt"><span style="font-weight:normal">UPI</span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal"> در مغز جنین شد</span></span></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="letter-spacing:-.2pt"><span style="font-weight:normal">.</span></span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Koodak""><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:11.0pt">Background</span></b><span style="font-size:11.0pt">: Uteroplacental insufficiency (UPI) by inducing oxidative stress in the fetal brain leads to disruption of its development. The present study evaluated the effects of Naringin on the antioxidant defense system in the fetal forebrain and prevention of the hippocampal damage following UPI. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:11.0pt">Materials and methods</span></b><span style="font-size:11.0pt">: 20 pregnant Wistar rats were randomly divided into 4 groups: control, UPI+Saline, UPI+Nar50 (UPI+naringin with a dose of 50 mg/kg) and UPI+Nar100 (UPI+ naringin with a dose of 100 mg/kg). In order to induce UPI, permanent occlusion of the anterior uterine vessels was performed on the embryonic day (ED) 18. Naringin and saline were gavagd from ED12-18. On the ED21, measuring the level of catalase (CAT), superoxide dismutase (SOD) and antioxidant capacity (TAC) by ELISA technique and malondialdehyde (MDA) by thiobarbituric acid method in the fetal forebrain and evaluating neuronal density in the CA1 and CA3 areas of the hippocampus were done. </span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:inter-ideograph"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:11.0pt">Results</span></b><span style="font-size:11.0pt">: A significant decrease in the brain activity of CAT, SOD, TAC and neuronal density in the CA1/CA3 areas of the hippocampus along with a significant increase in MDA was seen in the UPI+Saline group compared to the control group (p<0.05). While, a significant increase in CAT, SOD, TAC and CA1/CA3 neuronal density and a significant decrease in MDA was seen in the groups receiving Naringin compared to the UPI+Saline group (p<0.05).</span></span></span></span></span><br>
<span style="font-size:12pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:11.0pt"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:11.0pt">Conclusion</span></b><span style="font-size:11.0pt">: Administering Naringin before induction of UPI by strengthening the antioxidant system in the fetal forebrain caused the prevention of neurological complications caused by UPI in the fetal brain.</span></span></span></span></span></span><br>
محدودیت رشد درون رحمی, نارینجین, استرس اکسایشی, هیپوکامپ, موش صحرایی
Intrauterine growth restriction, Naringin, oxidative stress, hippocampus, rat
122
132
http://tmuj.iautmu.ac.ir/browse.php?a_code=A-10-727-9&slc_lang=fa&sid=1
Samireh
Nemati
سمیره
نعمتی
nemati.samireh@gmail.com
100319475328460011265
100319475328460011265
No
PhD Candidate in Cell and Developmental Biology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
دانشجوی دکتری زیستشناسی سلولی تکوینی، گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
Mohammad Amin
Edalatmanesh
محمد امین
عدالت منش
amin.edalatmanesh@gmail.com
100319475328460011266
100319475328460011266
Yes
of Physiology, Department of Biology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
دانشیار فیزیولوژی، گروه زیست شناسی، دانشکده علوم، واحد شیراز، دانشگاه آزاد اسلامی، شیراز،
Mohsen
Forouzanfar
محسن
فروزانفر
mforozanfar@yahoo.com
100319475328460011267
100319475328460011267
No
of Developmental Biology, Department of Biology, College of Sciences, Marvdasht Branch, Islamic Azad University, Shiraz, Iran
استادیار بیولوژی تکوینی، گروه زیست شناسی، دانشکده علوم، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران