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:: Volume 18, Issue 1 (Spring 2008) ::
MEDICAL SCIENCES 2008, 18(1): 1-6 Back to browse issues page
Prenatal diagnosis of fetal sex by molecular analysis of maternal plasma after 8 weeks of gestation
Mehrdad Hashemi 1, Ali Nazemi , Mahboubeh Safavi , Shahla Chaichian , Masoud Ghane , Shahrashoob Sharifi
1- , Hashemi_mehrdad@yahoo.com
Abstract:   (35035 Views)

Background: Prenatal diagnosis of fetal sex is usually performed by invasive methods such as sampling through amniocentesis or chorionic villus sampling. One potential non-invasive approach involves analysis of cell-free fetal DNA in maternal plasma or serum. The objective of our study was to investigate the feasibility of using fetal DNA in maternal plasma for prenatal diagnosis of fetal sex.
Materials and Methods: In this experimental study, a nested polymerase chain reaction (PCR) technique was developed for fetal SRY gene identification using cell-free fetal DNA in maternal plasma. Peripheral blood samples were obtained from 32 pregnant women at the gestational period from 8 to 13 weeks and cell-free DNA was extracted by the phenol/chloroform method from plasma. The nested PCR was carried out to amplify the fragment of SRY gene by two sets of PCR primer pairs. Analysis was then performed on the PCR product. Specifically, the presence of Y-chromosome sequences in maternal blood plasma indicates that the fetus is male, whereas lack of a signal will indicate that the fetus is female.
Results: Among the 32 pregnant women, SRY sequences were detected in 14 plasma samples after nested PCR amplification, while the 18 women bearing female fetuses had the negative results. The sensitivity of this technique was 87.5%.
Conclusion: The phenol/chloroform extraction of fetal DNA in maternal plasma is an effective and simple method, and the nested PCR amplification of SRY sequence is a convenient and low-cost approach for the non-invasive early prenatal diagnosis of fetal sex.

Keywords: Prenatal diagnosis, Fetal sex, nested PCR, SRY gene
Full-Text [PDF 194 kb]   (4781 Downloads)    
Subject: Epidemiology
Received: 2006/09/6 | Accepted: 2016/04/12 | Published: 2016/04/12
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Hashemi M, Nazemi A, Safavi M, Chaichian S, Ghane M, Sharifi S. Prenatal diagnosis of fetal sex by molecular analysis of maternal plasma after 8 weeks of gestation. MEDICAL SCIENCES 2008; 18 (1) :1-6
URL: http://tmuj.iautmu.ac.ir/article-1-123-en.html


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Volume 18, Issue 1 (Spring 2008) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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