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:: Volume 28, Issue 3 (spring 2018) ::
MEDICAL SCIENCES 2018, 28(3): 220-229 Back to browse issues page
Streptokinase enzyme extraction from Streptococcus agalactiae isolated from mucous membrane and investigation of fibrinolytic effects and its toxicity on healthy cells Hu02
Ali Akbar Hatami1 , Anosh Eghdami 2
1- 1MSc, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran , eghdami49@gmail.com
Abstract:   (5254 Views)
Background: Streptokinase, the most well-known fibrinolytic drug, has long been used in the treatment of myocardial infarction. This enzyme is produced from beta-hemolytic bacteria. In this study, the Streptokinase enzyme was extracted from Streptococcus agalactia strain with ATCC12386, isolated from the throat.
Materials and methods: After cultivating the bacteria in the Blood agar medium and evaluating their aggressive activity, the bacteria were transferred to the BHI broth medium and after incubation at 37 ° C for 24 hours, centrifuge was carried out at 4 ° C for 10000 g for 30 minutes. The contents of the tubes were separated by 0.45 micron acetate cellulose filter and the filters were considered as crude enzymes until fibrinolysis activity and non-toxicity test were performed.
Results: Among the extracted enzymes, the enzymes of the shamrock 2, 6, 10, 13, 14, 29 and 27 had fibrinolytic activity higher than 30% and extraction enzyme from the standard bacteria had less fibrinolysis activity. The toxicity of the extracellular enzyme was lower than that of other samples in 24 hours and 48 hours.
Conclusion: Extracting enzymes have a fibrinolysis effect, and this effect is higher in the bacteria isolated from the throat than the standard bacteria. The toxicity of the enzymes varies with time and concentration. It decreases with decreasing toxicity and increases with increasing time. And it can be described as a new, low-risk, and natural remedy for the elimination of blood clot sound.
 
Keywords: Streptococcus agalactiae, Streptokinase, Fibrinolytic effect, Normal fibroblast cells.
Keywords: Streptococcus agalactiae, Streptokinase, Fibrinolytic effect, Normal fibroblast cells.
Full-Text [PDF 287 kb]   (2555 Downloads)    
Semi-pilot: Experimental | Subject: Microbiology
Received: 2018/01/15 | Accepted: 2018/03/6 | Published: 2018/09/22
ENG-PDF [PDF 271 KB]  (124 Download)
References
1. Basheer N, Modawi A, Elamin HB, Ibrahim HM. A Potential New Isolate for Streptokinase Production. Int J Curr Microbiol App Sci 2015;4:380-7.
2. Nejadmoghaddam MR, Modarressi MH, Babashamsi M, Chamankhah M. Cloning and Overexpression of Active Recombinant Fusion Streptokinase: A New Approach to Facilitate Purification. Pak J Biol Sci 2007;10:2146-51. [DOI:10.3923/pjbs.2007.2146.2151]
3. Chakraborty C, Agoramoorthy G. A special report on India's biotech scenario: Advancement in biopharmaceutical and health care sectors. Biotechnology advances. 2010;28:1-6. [DOI:10.1016/j.biotechadv.2009.10.007]
4. Instruments BT, Winooski V. Serum albumin leads to false-positive results in the XTT and the MTT assay. Bio Techniques 2007;43:178-86. [DOI:10.2144/000112528]
5. Ahmad Gh, Kalin J. Study of adhesion and proliferation characteristics of different populations of epidermal cells of hair follicle in culture media.
6. Anarkulli C. An Investigation of a Method for Epithelial Cell Culture in the Skin of the Albino Rabbit. J Zanjan Univ Med Sci 1998;6:28-34.
7. Golshid Jashh, Parviz, Hamid Ghazan, Mansoureh M. Transferring the Enhanced Green Florescent Protein Gene to a Calf Spermatogonium Clone Using Turbofect.
8. Soltan Dallal MM, Yazdi MH, Hassan ZM, Holakuyee M, Abedi Mohtasab TP, Aminharaty F, et al. The effect of oral administration of Lactobacillus acidophilus on enhancing the immune system's efficiency and increasing the life span of mice with breast cancer. TUMJ 2010;67:753-8.
9. Arabi R, Rohavand F, Farzin, Norouzian, Sadeghi A, Rahedian M. et al. Cloning and expression of shortened and intact streptokinase molecules in Escherichia coli and assessment of their biological activity. Agricultural Biotechnology Journal 2011;2:55-67.
10. Sasirekha C, Ramya S, Balagurunathan R. Fibrinolytic enzyme from actinomycetes. J Pharm Res 2012;5:5457.
11. Jalalirad R, Kavianpour A, Beiroti A, Sepahi M, Tavakoli Zaniani P, Arsalani F. Exploration of Recombinant Streptokinase Degradation Products Under Various pH Reduction Conditions in Downstream Processing. Recent Pat Biotechnol 2015;9:139-44. [DOI:10.2174/187220830902160308194812]
12. Zia MA, Shahid M, Abdullah S, Faran Ge. Improved streptokinase production; uv irradiation of streptococcus equisimilis. TPMJ 2015;22:656-63.
13. Chandrasekaran SD, Vaithilingam M, Shanker R, Kumar S, Thiyur S, Babu V, et al. Exploring the in vitro thrombolytic activity of nattokinase from a New Strain Pseudomonas aeruginosa CMSS. Jundishapur J Microbiol 2015;8:e23567. [DOI:10.5812/jjm.23567]
14. Bhardwaj S, Angayarkanni J. Streptokinase production from Streptococcus dysgalactiae subsp. equisimilis SK-6 in the presence of surfactants, growth factors and trace elements. 3 Biotech 2015;5:187-93. [DOI:10.1007/s13205-014-0209-x]
15. Magalhaes V, Veiga-Malta I, Almeida MR, Baptista M, Ribeiro A, Trieu-Cuot P, et al. Interaction with human plasminogen system turns on proteolytic activity in Streptococcus agalactiae and enhances its virulence in a mouse model. Microbes Infect 2007;9:1276-84. [DOI:10.1016/j.micinf.2007.06.001]
16. Babu V, Subathra Devi C. In vitro thrombolytic activity of purified streptokinase extracted from Streptococcus equinus VIT_VB2 isolated from bovine milk. J Thromb Thrombolysis 2015;39:71-8. [DOI:10.1007/s11239-014-1093-2]
17. Dubey R, Kumar J, Agrawala D, Char T, Pusp P. Isolation, production, purification, assay and characterization of fibrinolytic enzymes (Nattokinase, Streptokinase and Urokinase) from bacterial sources. AJOL 2011;10:1408-20.
18. Karimi Z, Babashamsi M, Asgarani E, Niakan M, Salimi A. Fermentation, fractionation and purification of streptokinase by chemical reduction method. Iran J Microbiol 2011;3:42.
19. Zalas-Wiecek P, gospodarek E, Piecyk K. Influence of subinhibitory concentrations of cefotaxime, imipenem and ciprofloxacin on adhesion of Escherichia coli strains to polystyrene. Pol J Microbiol 2011;60:345-9.
20. Alam T, Khattak YJ, Beg M, Raouf A, Azeemuddin M, Khan AA. Diagnostic accuracy of ultrasonography in differentiating benign and malignant thyroid nodules using fine needle aspiration cytology as the reference standard. Asian Pac J Cancer Prev 2014;15:10039-43. [DOI:10.7314/APJCP.2014.15.22.10039]
21. Velumani S, Toh YX, Balasingam S, Archuleta S, Leo YS, Gan VC, et al. Low antibody titers 5 years after vaccination with the CYD-TDV dengue vaccine in both pre-immune and naive vaccinees. Hum Vaccines Immunother 2016;12:1265-73. [DOI:10.1080/21645515.2015.1126012]
22. Goyal D, Sahni G, Sahoo DK. Enhanced production of recombinant streptokinase in Escherichia coli using fed-batch culture. Bioresour Technol 2009;100:4468-74. [DOI:10.1016/j.biortech.2009.04.008]
23. Sriraman K, Jayaraman G. Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response. Appl Microbiol Biotechnol 2006;72:1202-9. [DOI:10.1007/s00253-006-0410-x]
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Hatami A A, Eghdami A. Streptokinase enzyme extraction from Streptococcus agalactiae isolated from mucous membrane and investigation of fibrinolytic effects and its toxicity on healthy cells Hu02 . MEDICAL SCIENCES 2018; 28 (3) :220-229
URL: http://tmuj.iautmu.ac.ir/article-1-1444-en.html


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Volume 28, Issue 3 (spring 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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